Interaction of fisetin with human serum albumin by fluorescence, circular dichroism spectroscopy and DFT calculations: binding parameters and conformational changes

Flavonoid aglycones can compete with Ochratoxin A for human serum albumin: A new possible mode of action

“…1). In almost all cases we found literature data of their binding to HSA [33][34][35][38][39][40][41][42][43]. However, no data were found for any trial using flavonoid aglycones in competitive OTA-HSA models.…”

Multiple Spectroscopic and Theoretical Approaches to Study the Interaction between HSA and the Antiparasitic Drugs: Benznidazole, Metronidazole, Nifurtimox and Megazol

“…42 Quantitatively, the α-helix percent decreases gradually from 63.1% in free HSA to 62.7, 61.4, 61.1 and 59.5% upon MTZ, BZL, MZ or NFX binding, respectively, at 208 nm. Similarly, at 222 nm the α-helix percent decreases gradually from 59.5% in free HSA to 58.9, 58.8, 58.2, and 57.4% upon MZ, MTZ, BZL or NFX binding, respectively.…”

A viscometric approach of pH effect on hydrodynamic properties of human serum albumin in the normal form

“…In recent years the studies mainly focus on the investigations of mechanisms of binding of HSA with different molecules and atoms, but are also directed at the determinations of the hydration, thermal stability, denaturation, interactions and other functional properties of HSA. The studies explore many different experimental techniques such as X-ray diffraction (He and Carter 1992;Olivieri and Craievich 1995;Bhattacharya et al 2000), infrared spectroscopy (Bramanti and Benedetti 1994), 1 H-NMR spectroscopy (Menon and Allen 1990;Baranowska and Olszewski 1996;Pouliquen and Gallois 2001), EPR spectroscopy (Junk et al 2011), phase-fluorometry (Buzády et al 2001), smallangle neutron scattering (Sjöberg and Mortensen 1994), electrospray mass spectrometry (Amoresano et al 1998), photon correlation spectroscopy (Sontum and Christiansen 1997), differential scanning calorimetry (Picó 1997), circular dichroism spectroscopy (Matei et al 2011), fluorescence (Vos et al 1987;Gelamo et al 2002;Kamal and Behere 2002) and viscometry (Monkos 2004). There are very few papers dealing with the influence of the pH of a solution on the physicochemical properties of HSA.…”