1967
DOI: 10.1016/0005-2736(67)90055-7
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Interaction of filipin and derivatives with erythrocyte membranes and lipid dispersions: Electron microscopic observations

Abstract: SUMMARYThe effect of the polyene antibiotic, filipin, on the morphology of erythrocyte membranes and lipid dispersions has been investigated with the electron microscope using negative staining (phosphotungstate). Filipin induces pit formation in rat and human erythrocytes; similar pits are also produced by the antibiotic in lecithincholesterol dispersions. Pit formation requires the presence of cholesterol. Evidence is presented which suggests that the light ring surrounding each pit may consist predominately… Show more

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Cited by 109 publications
(50 citation statements)
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“…Dispersions prepared by mixture of ethanolic solutions of the steroid with an excess of water have been observed by electron microscopy of negatively stained preparations to consist of "microcrystals" of various shapes and sizes (40,41 …”
Section: Resultsmentioning
confidence: 99%
“…Dispersions prepared by mixture of ethanolic solutions of the steroid with an excess of water have been observed by electron microscopy of negatively stained preparations to consist of "microcrystals" of various shapes and sizes (40,41 …”
Section: Resultsmentioning
confidence: 99%
“…Also a model proposed for the filipincholesterol complex is visualized as a complex of 15-25 nm diameter (1,3,14,32). This complex is assumed to be composed of two regular arrays of stacked filipin molecules in a lipid bilayer.…”
Section: Discussionmentioning
confidence: 99%
“…Filipin is an uncharged polyene with the highest sterol affinity. Filipin-cholesterol complexes are visualized as pits with negative staining electron microscopy [1] and as particles (100--240 i~ in diameter) in the hydrophobic core of the membrane, with freeze-fracture electron microscopy [2,3]. The filipin-sterol aggregates lead to a fragmentation of the membrane and cause release of all cytoplasmic components [4].…”
Section: Introductionmentioning
confidence: 99%