Interaction of filarial proteins on growth regulation of normal lung epithelial cells in vitro

Abstract

An in vitro model to examine the effects of filarial proteins on lung epithelial cells has been developed. Several of these proteins appear in circulation of infected individuals. A close association between tropical pulmonary eosinophilia (TPE) and filariasis has been reported by several workers. [3H]-thymidine studies do indicate that when optimum concentration of these filarial proteins were added to lung cultures in proliferating and basal/maintenance media a further increase in growth stimulation was observed early in culture. However, on longer exposures and at higher concentrations an inhibitory effect with distinct morphological changes were noted. The dual role of these proteins on lung epithelial cells in vitro may highlight the possibility of a direct interaction of these proteins with lung cells during disease also contributing to tissue damage.

Antiviral Evaluation of Herbal Drugs

“…The cell pellets are treated with 11% cold trichloroacetic acid to remove amino acid pools and dissolved in alkaline cupric sulfate and folin ciocalteau phenolic reagent. Folin's reagent and cupric sulfate together react with amino acid to give a blue color and this color intensity is proportional to the protein concentration, which can be measured colorimetrically (Maya et al, 1995).…”

Long‐term culture of porcine esophageal epithelial cells without use of feeder layers

“…Primary cultures have been established from the following tissues and animals: uro epithelial cells from dog (Bonar et al, 1977), rabbit (Atala et al, 1992(Atala et al, , 1993Truschel et al, 1999), rodents (Noguchi et al, 1990;Sterle, 1996) and pig (Guhe and Föllmann, 1994;Fujiyama et al, 1995), lung epithelial from rats (Maya et al, 1995), tracheal epithelial from hamsters (Regina et al, 2002) through a limited number of passages. Primary cultures have been established from the following tissues and animals: uro epithelial cells from dog (Bonar et al, 1977), rabbit (Atala et al, 1992(Atala et al, , 1993Truschel et al, 1999), rodents (Noguchi et al, 1990;Sterle, 1996) and pig (Guhe and Föllmann, 1994;Fujiyama et al, 1995), lung epithelial from rats (Maya et al, 1995), tracheal epithelial from hamsters (Regina et al, 2002) through a limited number of passages.…”

“…Efforts to culture epithelial cells have not been easy, as undifferentiated epithelial primary cultures soon become overgrown with fibroblasts. Primary cultures have been established from the following tissues and animals: uro epithelial cells from dog (Bonar et al, 1977), rabbit (Atala et al, 1992(Atala et al, , 1993Truschel et al, 1999), rodents (Noguchi et al, 1990;Sterle, 1996) and pig (Guhe and Föllmann, 1994;Fujiyama et al, 1995), lung epithelial from rats (Maya et al, 1995), tracheal epithelial from hamsters (Regina et al, 2002) through a limited number of passages. However, most of these cultures require the presence of feeder layers, lethally irradiated 3T3 fibroblasts being the most commonly used.…”