Interaction of epidermal growth factor with specific binding sites of enterocytes isolated from rat small intestine during development

Toyoda, Shigeru; Lee, Ping-Cheung; Lebenthal, Emanuel

doi:10.1016/0167-4889(86)90148-5

Cited by 43 publications

(18 citation statements)

References 21 publications

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“…1b). ×430 clusive localization in the present study of P2X 5 receptors in the membrane of the narrow stem, where nucleus and cell organelles are localized, suggests that nucleotides acting via P2X 5 receptors may be involved in goblet cell maturation, possibly in collaboration with growth factors such as epidermal growth factor (Toyoda et al 1986). This would be another example of this member of the family of ATP-gated ion channels (Collo et al 1996;Garcia-Guzman et al 1996) acting on peripheral tissues.…”

Section: Discussionmentioning

confidence: 82%

P2X receptors in the rat duodenal villus

Gröschel‐Stewart

Bardini

Robson

et al. 1999

Cell and Tissue Research

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Immunohistochemical techniques were performed on freshly frozen sections of the duodenum of the rat using specific polyclonal antibodies to unique peptide sequences of P2X1-7 receptors. Of the antibodies to the seven known P2X receptor subtypes that mediate extracellular signalling by nucleotides, three reacted with discrete structures in the duodenal villus of the rat. Anti-P2X1 reacted with the capillary plexus in the intestinal villus, which did not extend to the crypt region, suggesting that nucleotides may be involved in the uptake and transport of metabolites. Anti-P2X5 immunostained the membranes of the narrow "stem" of villus goblet cells, where the nucleus and cell organelles reside, possibly influencing synthesis and release of mucins. P2X7 receptor immunoreactivity was only seen in the membranes of enterocytes and goblet cells at the tip of the villus, where cells are exfoliated into the lumen, consistent with earlier findings that P2X7 is involved in apoptotic events. Thus, in complex structures such as the intestinal villus, purinoceptors appear to participate in several and diverse signalling functions.

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Section: Discussionmentioning

confidence: 82%

P2X receptors in the rat duodenal villus

Gröschel‐Stewart

Bardini

Robson

et al. 1999

Cell and Tissue Research

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“…Toyoda et al [41] have reported the down-regulation of EGF-R fol lowing milk-feeding, while Gallo-Payel et al [42] observed an increase in binding through out the suckling and weanling periods with attainment of adult binding at 26 days of age. Differences between those studies and our present data may reflect differences in the accessibility of E G F to the epithelium in vivo as compared to the in vitro methods used in other studies.…”

Section: Discussionmentioning

confidence: 99%

Epidermal Growth Factor in the Neonatal Mouse Salivary Gland and Kidney

et al. 1992

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In the adult mouse, epidermal growth factor (EGF) is synthesized in granular convoluted tubule (intralobular) duct cells of the submandibular gland and in distal tubule cells of the kidney. The presence of EGF in developing tissues and maternal milk and the localization of EGF receptors in developing tissues suggest a role for EGF in developmental processes. The primary aims of the present study were to: (1) localize EGF and EGF-binding sites in the kidney and submandibular gland during neonatal development and (2) to determine the effect of exogenously administered EGF on cell proliferation in these two developing organs. In the present study, EGF was localized by immunocytochemistry in granular convoluted tubule cells of the submandibular gland initially on day 21 after birth and in distal tubule cells of the kidney on postnatal day 6. EGF binding in the kidney decreased after birth with some localization to the glomerulus. In submandibular glands of newborn and 10-day-old mice, EGF-binding sites were associated with both acinar and duct cells with peak binding at 10 days postnatally. Submandibular glands from 20-day-old mice demonstrated primarily ductal EGF-binding sites. Exogenously administered EGF induced a mitogenic response in acinar and interlobular duct cells of submandibular glands during the first week after birth. EGF treatment during this period had an inhibitory effect on ³H-thymidine incorporation into cellular compartments in the developing kidney. The identification of EGF-binding sites in the kidney and submandibular gland before the presence of EGF suggests that an EGF-like molecule such as transforming growth factor α (TGF-α) may be present as a potential ligand in these organs. In order to assess this possibility, developing kidneys and submandibular glands were stained with anti-TGF-α. These immunocytochemical studies localized TGF-α to the proximal tubule of the kidney and immature acinar cells of the newborn mouse. Our data strongly support an autocrine, juxtacrine or paracrine role for EGF and/or TGF-α in the regulation of cell proliferation and cytodifferentiation in the kidney and submandibular gland.

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“…This, in turn, has led to a number of studies acknowledging the presence of specific EGF receptors in gut epithelia during the many phases of rodent development, namely fetal, suckling, and postsuckling periods (10)(11)(12)(13)(14). On the other hand, very little data exist concerning the effect of EGF on the developing colon.…”

mentioning

confidence: 97%

Epidermal Growth Factor Receptors during Postnatal Development of the Mouse Colon*

1987

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The present study was undertaken to establish the postnatal profile of specific epidermal growth factor (EGF) binding in the maturing mouse colon, with particular emphasis on possible regional differences between both proximal and distal colonic binding patterns vs. those of the small intestine. Binding studies using [125I]EGF were performed on isolated epithelial cells obtained from 2-, 5-, 9-, 16-, and 22-day-old mice as well as adults. At 2 days, cells isolated from the entire colon bound 4 times more [125I]EGF than did corresponding intestinal cells, whereas between the ages of 5 days to adult, colonic cells bound between 1.7-2.5 times more labeled EGF than their intestinal counterparts. The immature colon already exhibited maximal binding after birth as opposed to the small intestine where binding only reached maximal values by the third week of life. Comparison between the proximal and distal colon in 9-, 16-, and 22-day-old mice revealed a further 2-fold increase in EGF binding in the distal colon compared to that in the proximal colon. Scatchard plots of [125I]EGF displacement by native EGF in both proximal and distal colonic segments also revealed the presence of two classes of binding sites, with high affinity constants (K1) significantly greater in the distal colon. These results demonstrate for the first time not only the continued presence of EGF receptors in mouse colonic epithelium, but also significant regional differences in EGF-binding capacity within the digestive tract throughout the postnatal period.

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Interaction of epidermal growth factor with specific binding sites of enterocytes isolated from rat small intestine during development

Cited by 43 publications

References 21 publications

P2X receptors in the rat duodenal villus

P2X receptors in the rat duodenal villus

Epidermal Growth Factor in the Neonatal Mouse Salivary Gland and Kidney

Epidermal Growth Factor Receptors during Postnatal Development of the Mouse Colon*

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