Interaction of elements of the golgi apparatus with microtubules

Cytoskeletal control of centrioles movement during the establishment of polarity in Madin-Darby canine kidney cells.

“…For example, kinesin will move towards the plus end of microtubules, whereas MAPle moves towards the minus end (Paschal and Vallee, 1987;Vale, 1987). Therefore, the location of a nucleating site in the cell determines the orientation of the microtubules in the cytoplasm and, as a consequence, the vectorial movement as well as the steady state position of organelles such as the Golgi apparatus, that slide along mierotubules in one direction (for review, see Thyberg and Moskalewski, 1985;Kreis et al, 1988). Actin microfilaments can also be oriented in the cell by having the barbed end bound to the junctional plaque for example (Begg et al, 1978).…”

Regulation of microtubule dynamics and nucleation during polarization in MDCK II cells.

“…Indeed, they are by essence unstable (Mitchison and Kirschner, 1984) and in equilibrium with a pool of soluble subunits in the cytoplasm (Inout, 1981). They are polar and this polarity is what determines the positioning of the Golgi apparatus, for example (Bacallao et al, 1989;Kreis et al, 1988;Tassin et al, 1985b;Thyberg and Moskalewski, 1985;Vale, 1987). Therefore, to understand how the Golgi apparatus and other cytoplasmic organelles are positioned in different cells, it is essential to understand how microtubule polarity is oriented and to identify the signals controlling this orientation.…”