Interaction of Doxorubicin and Dipalmitoylphosphatidylcholine Liposomes

Abstract

The interaction between doxorubicin (DOX), an anthracycline antibiotic frequently used in chemotherapy, and zwitterionic dipalmitoylphosphatidylcholine (DPPC) was investigated using Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC), and rheological measurements. FTIR results showed that DOX shifted the wavenumber of the PO(2)(-) band for pure DPPC to a higher wavenumber. This may have been because of the strong interactions between the NH(3)(+) group in DOX and the phosphate (PO(2)(-)) group in the polar head of DPPC. The main transition temperature of DPPC liposomes was slightly shifted to a lower temperature for DPPC liposome-encapsulated DOX. This suggested that DOX had a significant effect on the acyl chains in the DPPC bilayers, and that its presence decreased the transition cooperativity of lipid acyl chains. There was also the appearance of an additional transition peak at nearly 136 °C for the DPPC/DOX sample. These interactions between DOX and DPPC phospholipid would cause a decrease in the DPPC liposomes plastic viscosity and increase membrane fluidity. A better understanding of the interactions between DOX and lipid bilayers could help in the design and development of improved liposomal drug delivery systems.

Ehrlich tumor inhibition using doxorubicin containing liposomes

“…Anthracycline-based antibiotics such as doxorubicin (DOX), pirarubicin and daunorubicin have found widespread application in cancer chemotherapy. DOX is an anthracycline commonly used to treat several solid tumors, acute leukemia and malignant lymphoma (Kawano et al, 2003; Daemen et al, 1997; Mady et al, 2012). DOX can inhibit the replication process of cells by crossing the tumor cellular membrane and intercalating into the base pairs of DNA (Shi et al, 1993).…”

Interaction between Antibacterial Peptide Apep10 and Escherichia coli Membrane Lipids Evaluated Using Liposome as Pseudo-Stationary Phase

“…The different phospholipid compositions of the bilayers of the liposomes may contribute to these differences in interaction. Generally, positively charged ABPs interact more strongly with negatively charged membranes than with neutral membranes [7, 8, 24, 25]. However, there have been few reported comparisons between endogenous bacterial lipids and negatively charged phospholipids, which were most commonly used to mimic the cell membrane.…”

Nanolipodendrosome-loaded glatiramer acetate and myogenic differentiation 1 as augmentation therapeutic strategy approaches in muscular dystrophy

“…Inflammation, along with muscular degeneration, causes intensification of the muscle pathobiology state by destroying the remaining tissue and preparing it to rebuild. [1][2][3][15][16][17][18][19][20][21][22][23][24][25][26][27] For instance, in an mdx mice model of Duchenne MD (DMD), T-cells had a major part in skeletal muscle fibrogenesis and death. [17][18][19][20][21] Additionally, two fibrosis markers -fibronectin and vimentin -had a significant increase in comparison with normal models among patients suffering from DMD compared with healthy humans.…”

“…On the contrary, recovery factors of MyoD and Myogenin reduced in value. [17][18][19][20][21][22][23][24][25][26][27] Myogenic differentiation 1 (MyoD) is a key protein regulating muscular differentiation. MyoD expression is necessary as one of the primary myogenic regulatory factors in transforming satellite cells into myoblasts.…”

“…Among the other characteristics of this nanoparticle are controlled release and amplification. 18,20,22,25 The efficiency of the drug delivery can be increased by connecting other nanocarriers to the liposomes. Nanolipodendrosome is a type of dendritic family formed by connecting dendritic nanoparticles to liposomes.…”