Interaction of dolichol and dolichyl phosphate with phospholipid bilayers

Vigo, Carmen; Grossman, Steven H.; Drost-Hansen, W.

doi:10.1016/0005-2736(84)90295-5

Cited by 50 publications

(24 citation statements)

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“…The apparently modest decrease in DPH fluorescence anisotropy (r) observed in polyprenol-deficient thylakoids indicates that polyprenols have an ordering effect on the thylakoid membrane bilayer. Such changes in DPH polarization are typically observed in vitro only when millimolar quantities of membranealtering agents, such as sterols, SDS, or benzyl alcohol are incorporated into model membrane systems (Yamamoto et al, 1981;Vigo et al, 1984;Popova and Hincha, 2007;Lin et al, 2011). Our observations with polyprenol-deficient thylakoids are therefore striking when considering that on a molar basis, polyprenol abundance in thylakoid membranes is approximately an order of magnitude lower than in the model membranes described above.…”

Section: Discussionmentioning

confidence: 58%

“…These compounds are believed to intercalate into only one leaflet of the bilayer, perpendicular to its plane, and are orientated in such a way that their unusually long poly-cis isoprenoid moiety modulates fatty acyl chain motion via hydrophobic interactions (Zhou and Troy, 2003). In these systems, polyprenols increase membrane ionic permeability and enhance membrane fluidity (Vigo et al, 1984;Valtersson et al, 1985;Janas et al, 1994;Ciepichal et al, 2011). Our results indicate an opposite effect: our anisotropy measurements revealed that thylakoid membranes deficient in polyprenols actually exhibited a more "fluid," or perhaps disordered, state.…”

Section: Discussionmentioning

confidence: 99%

“…The incorporation of polyprenols into model membrane systems in vitro is well known to alter their membrane physico-chemical properties (Vigo et al, 1984;Valtersson et al, 1985;Schroeder et al, 1987;Janas et al, 1994;Wang et al, 2008;Sévin and Sauer, 2014). However, in vivo evidence to support these observations is lacking.…”

Section: Polyprenol Deficiency Alters Thylakoid Membrane Dynamicsmentioning

confidence: 99%

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Polyprenols Are Synthesized by a Plastidial cis-Prenyltransferase and Influence Photosynthetic Performance

et al. 2017

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Plants accumulate a family of hydrophobic polymers known as polyprenols, yet how they are synthesized, where they reside in the cell, and what role they serve is largely unknown. Using Arabidopsis thaliana as a model, we present evidence for the involvement of a plastidial cis-prenyltransferase (AtCPT7) in polyprenol synthesis. Gene inactivation and RNAi-mediated knockdown of AtCPT7 eliminated leaf polyprenols, while its overexpression increased their content. Complementation tests in the polyprenol-deficient yeast Δrer2 mutant and enzyme assays with recombinant AtCPT7 confirmed that the enzyme synthesizes polyprenols of ;55 carbons in length using geranylgeranyl diphosphate (GGPP) and isopentenyl diphosphate as substrates. Immunodetection and in vivo localization of AtCPT7 fluorescent protein fusions showed that AtCPT7 resides in the stroma of mesophyll chloroplasts. The enzymatic products of AtCPT7 accumulate in thylakoid membranes, and in their absence, thylakoids adopt an increasingly "fluid membrane" state. Chlorophyll fluorescence measurements from the leaves of polyprenol-deficient plants revealed impaired photosystem II operating efficiency, and their thylakoids exhibited a decreased rate of electron transport. These results establish that (1) plastidial AtCPT7 extends the length of GGPP to ;55 carbons, which then accumulate in thylakoid membranes; and (2) these polyprenols influence photosynthetic performance through their modulation of thylakoid membrane dynamics.

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Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Section: Polyprenol Deficiency Alters Thylakoid Membrane Dynamicsmentioning

confidence: 99%

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Polyprenols Are Synthesized by a Plastidial cis-Prenyltransferase and Influence Photosynthetic Performance

et al. 2017

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“…Moreover, dolichols may affect GABA uptake and binding by changing the organization, dynamics, and permeability of the membranes (Wood et al, 1986;Schroeder et al, 1987). It has been reported that free dolichols (0-1 mmol%) dramatically fluidize artificial model membranes and promote the formation of nonbilayer hexagonal phase II form in phospholipids, such as phosphatidylethanolamine (Vigo et al, 1984;Valtersson et al, 1985;Wood et al, 1986). Further studies-of Schroeder et al (1987) confirmed that some of these findings are also relevant to SPM, which is a biological membrane.…”

Section: Discussionmentioning

confidence: 90%

Docichol alters GABA uptake and high affinity binding of agonist to rat brain synaptic plasma membranes

Strosznajder

Samochocki

1989

Molecular and Chemical Neuropathology

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The effects of dolichol on high affinity [3H]muscimol binding to synaptic plasma membrane (SPM) and [3H]GABA uptake into synaptosomes from rat brain were analyzed. Membranes were enriched with dolichol, by preincubation, in the presence of bovine serum albumin (BSA) as a vehicle (40-100 micrograms of dolichol/mg protein + 5% BSA). The rate of dolichol incorporation into the membrane was determined using [1-3H]dolichol C95, and it was in the range of 5-7 nmol/mg protein/h for synaptosomes and SPM, respectively. The uptake of [3H]GABA into synaptosomes enriched with dolichol decreased significantly by about 30%. Dolichol alone added into the incubation medium produced only a negligible effect. Specific binding of [3H]muscimol, which was higher than 90% of total binding, was significantly reduced to SPM enriched with dolichol as compared to controls. The Kd of the high affinity sites was significantly elevated by about 30% in SPM enriched with dolichol (10.8 +/- 0.3 nM vs 7.3 +/- 0.2 nM in control). This difference was more pronounced for SPM isolated from cerebellum (Kd increased by about 50%). The Bmax value was not changed. Dolichol alone did not alter the agonist binding. These results indicate for the first time that the higher level of dolichol in SPM might influence the GABAergic transmission system. An increase in dolichols in membranes may be an important factor in the decline of brain function during aging.

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“…Accumulation of free dolichol in human and mouse brain is associated with aging [3-61 and with neuronal degenerative disorders such as neuronal ceroid-lipofuscinosis [3] and Alzheimer's disease [7]. Several reports using electron spin resonance probes [8], fluorescence probes 191, differential scanning calorimetry [9,10], "P-NMR [lo], freeze fracture electron microscopy [lo] and X-ray scattering [lo] indicate that free dolichol (O-l mol%) dramatically fluidizes artificial model membranes and promotes formation of the nonbilayer hexagonal phase II form in phospholipids such as phosphatidylethanolamine.…”

Section: Introductionmentioning

confidence: 99%

Dolichol alters dynamic and static properties of mouse synaptosomal plasma membranes

et al. 1986

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Dolichols are isoprenologues that are found in almost all tissues and whose biochemical function, aside from dolichol phosphate precursors, is not known. In addition, an understanding of the organizational and dynamic properties of dolichols in biological membranes has not been forthcoming. The purpose of the experiments reported here were to examine the effects of dolichol on the physical properties of mouse synaptic plasma membranes (SPM). Differential polarized phase fluorometry indicated that dolichol both fluidized and regidified SPM. Membrane areas detected by diphenylhexatriene and trans-parinaric acid were selectively fluidized and rigidified, respectively. It also was found that the spin label, 5-doxyl stearic acid indicated that dolichol reduced membrane fluidity. These results report for the first time a stuctural effect of dolichol on a biological membrane. Dolichol Fluorometry ESR

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Interaction of dolichol and dolichyl phosphate with phospholipid bilayers

Cited by 50 publications

References 24 publications

Polyprenols Are Synthesized by a Plastidial cis-Prenyltransferase and Influence Photosynthetic Performance

Polyprenols Are Synthesized by a Plastidial cis-Prenyltransferase and Influence Photosynthetic Performance

Docichol alters GABA uptake and high affinity binding of agonist to rat brain synaptic plasma membranes

Dolichol alters dynamic and static properties of mouse synaptosomal plasma membranes

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