Interaction of Different Types of Cells on Polymer Surfaces with Wettability Gradient

Lee, Jin Ho; Khang, Gilson; Lee, Jin Whan; Lee, Hai Bang

doi:10.1006/jcis.1998.5688

Cited by 439 publications

(353 citation statements)

References 35 publications

(21 reference statements)

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“…Surface wettability can be altered in many ways such as plasma treatment, surface coating, introduction of hydrophilic or hydrophobic groups by self-assembled monolayers (SAMs) 11,12,14,[22][23][24][25][26] . Among these methods, plasma treatment (radio frequency glow discharge) has a long history of modifying surface wettability.…”

Section: Discussionmentioning

confidence: 99%

“…A large number of studies indicated that cells tended to attach onto hydrophilic surfaces than onto hydrophobic surfaces [6][7][8][9][10] . On the other hand, it has been reported that cells adhered and proliferated at the highest rate when cultured on a substrate with hydrophobic surfaces 11 or a contact angle of around 70 degrees, and cell adhesion and proliferation rates were lower on more hydrophilic or more hydrophobic surfaces 3,12,13 . These confusing results were considered to be based on the different materials used, different surface topographies employed, and the narrow ranges of surface wettability employed 11,14,15 .…”

Section: Introductionmentioning

confidence: 99%

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Adhesion of mouse fibroblasts on hexamethyldisiloxane surfaces with wide range of wettability

et al. 2006

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Surface wettability is an important physicochemical property of biomaterials, and it would be more helpful for understanding this property if a wide range of wettability are employed. This study focused on the effect of surface wettability on fibroblast adhesion over a wide range of wettability using a single material without changing surface topography. Plasma polymerization with hexa methyldisiloxane followed by oxygen (O 2 ) plasma treatment was employed to modify the surfaces. The water contact angle of sample surfaces varied from 106 degrees (hydrophobicity) to almost 0 degrees (super-hydrophilicity). O 2 -functional groups were introduced on polymer surfaces during O 2 -plasma treatment. The cell attachment study confirmed that the more hydrophilic the surface, the more fibroblasts adhered in the initial stage that includes on super-hydrophilic surfaces. Cells spread much more widely on the hydrophilic surfaces than on the hydrophobic surfaces. There was no significant difference in fibroblast proliferation, but cell spreading was much greater on the hydrophilic surfaces. These findings suggest the importance of the surface wettability of biomaterials on initial cell attachment and spreading. The degree of wettability should be taken into account when a new biomaterial is to be employed. Further research of surface wettability on adhesive molecules is necessary for a better understanding of this property.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Adhesion of mouse fibroblasts on hexamethyldisiloxane surfaces with wide range of wettability

et al. 2006

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“…Accordingly, the Ti substrates coated with collagen/HA nanocomposite coatings were considered to be more promising than the collagen-coated Ti equivalents in the bone regeneration field. When comparing the nanocomposite coatings, the cells on the COL-30%HA coatings exhibited a lower proliferation level than those on the COL-20%HA coatings, and this was partially due to the relatively rougher and more hydrophobic surface of the former [27,28].…”

Section: In Vitro Evaluation Of Cell Responsesmentioning

confidence: 99%

Bioactive nanocomposite coatings of collagen/hydroxyapatite on titanium substrates

Teng

Lee

Park

et al. 2008

J Mater Sci: Mater Med

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Collagen/hydroxyapatite (HA) nanocomposite thin films containing 10, 20, and 30 wt.% HA were prepared on commercially pure titanium substrates by the spin coating of their homogeneous sols. All of the nanocomposite coatings having a thickness of *7.5 lm exhibited a uniform and dense surface, without any obvious aggregation of the HA particles. A minimum contact angle of 36.5°w as obtained at 20 wt.% HA, suggesting that these coatings would exhibit the best hydrophilicity. The in vitro cellular assays revealed that the coating treatment of the Ti substrates favored the adhesion of osteoblast-like cells and significantly enhanced the cell proliferation rate. The cells on the nanocomposite coatings expressed much higher alkaline phosphatase (ALP) levels than those on the uncoated Ti substrates. Increasing the amount of HA resulted in a gradual improvement in the ALP activity. The nanocomposite coatings on Ti substrates also exhibited much better cell proliferation behaviors and osteogenic potentials than the conventional composite coatings with equivalent compositions, demonstrating the greater potential of the former as implant materials for hard tissue engineering.

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“…Its importance in the design of implantable scaffolds was first reported by Weiss in 1960, 16 later confirmed by many others. 5,[17][18][19][20][21][22] Since cells effectively adhered onto polymer surfaces with moderate wettability as demonstrated by water contact angles of 40-70 degress, [23][24][25][26][27] it is clear that the wettability of a biomaterial surface plays a critical role in cell attachment and proliferation. [28][29][30] In addition, it has been shown that most human bone marrow stromal cells attached to hydrophobic (HP) surfaces had smaller surface contact areas, resulting in a more rounded cell morphology.…”

Section: Introductionmentioning

confidence: 99%

Carbon nanotubes impregnated with subventricular zone neural progenitor cells promotes recovery from stroke

Moon¹,

Kim²,

Bokara³

et al. 2012

IJN

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The present in vivo study was conducted to evaluate whether hydrophilic (HL) or hydrophobic (HP) carbon nanotubes (CNTs) impregnated with subventricular zone neural progenitor cells (SVZ NPCs) could repair damaged neural tissue following stroke. For this purpose, stroke damaged rats were transplanted with HL CNT-SVZ NPCs, HP CNT-SVZ NPCs, or SVZ NPCs alone for 1, 3, 5, and 8 weeks. Results showed that the HP CNT-SVZ NPC transplants improved rat behavior and reduced infarct cyst volume and infarct cyst area compared with the experimental control and the HL CNT-SVZ NPC and SVZ NPCs alone groups. The transplantation groups showed an increase in the expression of nestin (cell stemness marker) and proliferation which was evident with the increased number of doublecortin and bromodeoxyuridine double-stained immunopositive cells around the lesion site. But, these effects were more prominent in the HP CNT-SVZ NPC group compared with the other transplantation groups. The HP CNT-SVZ NPC and HL CNT-SVZ NPC transplants increased the number of microtubule-associated protein 2 (marker for neurons) and decreased the number of glial fibrillary acidic protein (marker for astroglial cells) positive cells within the injury epicenter. The majority of the transplanted HP CNT-SVZ NPCs collectively broadened around the ischemic injured region and the SVZ NPCs differentiated into mature neurons, attained the synapse morphology (TUJ1, synaptophysin), and decreased microglial activation (CD11b/c ). For these reasons, this study provided the first evidence that CNTs can improve stem cell differentiation to heal stroke damage and, thus, deserve further attention.

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Interaction of Different Types of Cells on Polymer Surfaces with Wettability Gradient

Cited by 439 publications

References 35 publications

Adhesion of mouse fibroblasts on hexamethyldisiloxane surfaces with wide range of wettability

Adhesion of mouse fibroblasts on hexamethyldisiloxane surfaces with wide range of wettability

Bioactive nanocomposite coatings of collagen/hydroxyapatite on titanium substrates

Carbon nanotubes impregnated with subventricular zone neural progenitor cells promotes recovery from stroke

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