Interaction of cultured human endothelial cells with polymeric surfaces of different wettabilities

Abstract

The in vitro interaction of human endothelial cells (HEC) and polymers with different wettabilities in culture medium containing serum was investigated. Optimal adhesion of HEC generally occurred onto moderately wettable polymers. Within a series of cellulose type of polymers the cell adhesion increased with increasing contact angle of the polymer surfaces. Proliferation of HEC occurred when adhesion was followed by progressive flattening of the cells.Our results suggest that moderately wettable polymers exhibit a serum and/or cellular protein adsorption pattern that is favourable for growth of HEC. When serum is present in the culture medium, cell adhesion to wettable substrates seems to be influenced by the adsorption of serum proteins onto these substrates5. *Hospital Ziekenzorg, Enschede, The Netherlands. If cell adhesion is studied in serum-free medium, the absorption of protein(s) originating from the cells onto wettable substrates may be of importance6.Like other cell types endothelial cells can be cultured on glass and wettable tissue culture polystyrene, which is a glow discharge treated polystyrene. Bovine endothelial cells have been reported to grow on polyacrylamide beads' and on sulphonated polystyrene beads'. Furthermore, these cells grow upon crosslinked dextran beads precoated with collageng. The presence of collagen is also needed for growth upon the very hydrophilic polyHEMA". Poor growth of bovine endothelial cells was found upon the polyurethane Biomer, unless Biomer was precoated with gelatin or an extracellular matrix' ' Little is known, however, about the behaviour of (human) endothelial cells and other materials. We have previously presented preliminary data about the interaction of cultured human endothelial cells (H EC) with several polymeric surfaces".In the present study, we have examined the in vitro adhesion and proliferation of HEC on(to) a number of polymers with different wettabilities in culture medium containing serum. MATERIALS AND METHODS Cell culture

Species and Density of Implant Surface Chemistry Affect the Extent of Foreign Body Reactions

“…This behavior was attributed more to the hydrophilicity than the effect of the functional group. 79,81 The reason for relatively insignificant effects of functional group density on in vivo tissue responses, as observed in the present study, is not clear at this moment. It is possible that once a threshold density of a particular functional group is made available, further increases in these groups are relatively ineffective in that an essentially similar initial biomolecule surface-interaction, ultimately responsible for the capsule formation, cell infiltration, etc., dominates under in vivo conditions, independent of functional group concentrations, independent on the surface chemistry.…”

“…These results contradict many in vitro observations which found cell adhesion to be directly correlated to the density of functionalization. 79,81 Furthermore, the density of surface functional groups has also been shown to increase the adhesion of cells to tissue culture plate surfaces. In an earlier study, the density of negatively charged surface functionality was varied along the length of the implant and then cultured with hamster ovary cells.…”

Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro☆

“…2 is not frequently measured, especially the early attachment phase characterized by t 1/2 and %I max, because cell-enumeration protocols are quite labor intensive (there are a variety of cell-enumeration methods available including dye techniques [67][68][69][70][71][72][73][74], autoradiography [75], light and electron microscopy [76][77][78], Coulter counting [79], hemocytometry [80], spectrometry [81,82], nuclei number [83], total DNA [84], total protein concentration [78,85] that may or may not give similar results, depending on cell number and specific experimental conditions). Instead, a variety of experimental shortcuts are taken, such as measuring attached-cell-number after some arbitrary cell-surface contact time [86][87][88][89].…”

Convenient surface functionalization of whole-Teflon chips with polydopamine coating

“…46 Generally, non-wetting substrates, such as PE and PTFE, are not favorable for cell adhesion and proliferation. 47 In our previous work, 8 cells adhered to the surface of PFA channels that were pretreated with concentrated sodium hydroxide overnight, which is time consuming with involvement of corrosive reagents. Our above results proved that PDA coating can greatly improve the wetting properties of FEP surface, but the cell viability and activity on PDA-coated FEP channels are still needed to be investigated.…”