Interaction of coumarin with calf thymus DNA: Deciphering the mode of binding by in vitro studies

Synthesis, DNA-Binding and Antibacterial Activity of the Cell-Penetrating Peptide HIV-1 Tat (49-57)

“…In this work, EB was used as the fluorescence probe. The fluorescence intensity of DNA can be enhanced after interaction with EB because EB can intercalate into the DNA base pairs [25][26][27] . Fig.…”

Studying non-covalent drug–DNA interactions

“…Ligands are then made flexible to attain different conformations in order to predict the best fit orientation for binding to DNA helix and best energy docked structures are further analysed. Several runs of search are carried out to determine the best ligand-receptor orientation [93]. There are various modelling theories and algorithms that have been evolved in the last decades.…”

Interaction of β-Carbolines with DNA: Spectroscopic Studies, Correlation with Biological Activity and Molecular Docking

“…Thus, the binding mode between the β-carboline alkaloids with ctDNA was proposed according to three strategies: (i) evaluation of the effect of KI quenching, (ii) competition with ethidium bromide, and (iii) the profile of the DNA thermal denaturation. 42,43 The iodide ion is capable of promoting the dynamic quenching of fluorescent molecules in solution. However, the molecule must be accessible for the interaction that occurs with the iodide ion.…”

“…Due to the interleaving process of small molecules within the double helix of DNA, there is an increase in T m of DNA above 5 °C (∆T m > 5). 43,44 This positive variation in denaturation temperature is attributed to increased stability of the DNA double helix by intercalation of small molecules between the chain bases. However, when the molecule does not interact by intercalation, it is not observed significant increase in T m value.…”