Interaction of colocalized neuropeptides: functional significance in the circadian timing system

Albers, H. Elliott; Liou, Shyh Yuh; Stopa, Edward G.; Zoeller, R. Thomas

doi:10.1523/jneurosci.11-03-00846.1991

Cited by 202 publications

(136 citation statements)

References 23 publications

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“…Combined application of VIP, PHI, and GRP induces a phase shift of locomotor activity rhythm and a change in electrical activity (Albers et al 1991).…”

Section: Discussionmentioning

confidence: 97%

Peptide Expression in GABAergic Neurons in Rat Suprachiasmatic Nucleus in Comparison with Other Forebrain Structures: A Double Labeling In Situ Hybridization Study

Tanaka

Matsuda

Shigeyoshi

et al. 1997

J Histochem Cytochem.

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SUMMARYWe investigated the characteristics of GABAergic neurons in the rat suprachiasmatic nucleus (SCN) in normal untreated rats by examination of co-expressed peptides. We adopted double labeling in situ hybridization using a digoxigenin-labeled glutamic acid decarboxylase (GAD) riboprobe and 35 S-labeled peptide riboprobes. GAD mRNA-positive neurons were distributed throughout the SCN from the rostral to the caudal pole. In the dorsomedial part of the SCN, most GAD mRNA-positive neurons co-expressed arginine vasopressin mRNA. In the ventrolateral part of the SCN, about two thirds of GAD mRNApositive neurons co-expressed vasoactive intestinal peptide (VIP) mRNA. Co-expression of GAD and somatostatin mRNA was observed in virtually all neurons of the intermediate part of the SCN. In contrast, these peptidergic traits were poorly expressed in hypothalamic GABAergic neurons outside the SCN. Vasopressin mRNA-positive cells in the supraoptic nucleus did not express GAD mRNA, and co-expression of somatostatin mRNA and GAD mRNA was rare in the periventricular hypothalamic nucleus. Similarly, the VIP mRNA co-expression ratio of GABAergic neurons in the cerebral cortex was far lower than that in the SCN.

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“…Combined application of VIP, PHI, and GRP induces a phase shift of locomotor activity rhythm and a change in electrical activity (Albers et al 1991).…”

Section: Discussionmentioning

confidence: 97%

Peptide Expression in GABAergic Neurons in Rat Suprachiasmatic Nucleus in Comparison with Other Forebrain Structures: A Double Labeling In Situ Hybridization Study

Tanaka

Matsuda

Shigeyoshi

et al. 1997

J Histochem Cytochem.

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“…CalB (Bryant et al, 2000) and GRP (Aioun et al, 1998) cells receive retinal terminals and presentation of a light pulse during the night induces c-Fos in about 80% of the CalB cells (Silver et al, 1996), 78% of the GRP cells and 60% of the VIP cells (Aioun et al, 1998). It is also known that administration of GRP (McArthur et al, 2000) or coadministration of VIP, peptide histidine isoleucine and GRP (Albers et al, 1991) induce phase-dependent resetting of the SCN that resembles the lightinduced phase response curve. Finally, administration of CalB antisense blocks lightinduced phase shifts (Hamada et al, 2000).…”

Section: Colocalization Of Peptides In the Calb Subnucleusmentioning

confidence: 99%

Calbindin-D28K cells selectively contact intra-SCN neurons

et al. 2002

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Calbindin-D 28K -immunoreactive cells are tightly packed within a discrete region of the caudal aspect of the suprachiasmatic nuclei of hamsters. These cells receive direct retinal input and are Fos-positive in response to a light pulse. Knowledge of their afferent and efferent connections is necessary to understand suprachiasmatic nucleus organization. The first aim of the present study is to identify interconnections between calbindin and other peptidergic cells of the suprachiasmatic nuclei, using epi-and confocal microscopy and intra-suprachiasmatic nucleus tract tracing. The results indicate that essentially all calbindin cells receive numerous appositions from vasoactive intestinal polypeptide (VIP), neuropeptide Y and serotonin fibers and that most receive appositions from gastrin releasing peptide (GRP) and cholecystokinin (CCK) fibers. Reciprocal connections are seen from VIP, GRP and CCK cells but surprisingly, not from dorsomedial vasopressin cells. Injection of biotinylated dextran amine into the suprachiasmatic nucleus indicates that the ventrolateral suprachiasmatic nucleus projects to the entire nucleus, while the dorsal and medial regions of the suprachiasmatic nucleus project densely to most of the nucleus, except to the calbindin region. Analysis of colocalization of the peptides in the calbindin cell region shows that 91% of the substance P cells, 42% of the GRP cells and 60% of the VIP cells in the calbindin subnucleus coexpress calbindin-D 28K .Our results reveal a highly specialized topographical organization of connections among suprachiasmatic nucleus cells. Keywords suprachiasmatic nucleus; calbindin; vasopressin; confocal microscopy; tract tracingThe suprachiasmatic nuclei (SCN) are the locus of the brain clock regulating circadian rhythms in mammals (reviewed in Silver and Moore, 1998). In rodents, these paired hypothalamic nuclei are each composed of about 8000 neurons that are heterogeneous in their content of neuronal antigens (van den Pol, 1980;van den Pol and Tsujimoto, 1985). Some of the neuropeptides that have been identified to date in the rodent SCN are vasopressin (AVP), vasoactive intestinal polypeptide (VIP), peptide histidine isoleucine, gastrin releasing peptide (GRP), sub-stance P (SP), cholecystokinin (CCK) and somatostatin. We have reported that a subregion in the caudal SCN is characterized by the (Silver et al., 1996). These cells are densely packed, receive direct retinal synaptic input (Bryant et al., 2000) and are responsive to photic stimuli, with about 80% of the cells showing Fos-like immunoreactivity (ir) following a light pulse given during subjective night (Silver et al., 1996). The foregoing evidence indicates these CalB cells are on the input pathway for photic stimuli reaching the SCN.The function of this subregion of the SCN has been examined in hamsters. Animals with lesions that destroyed the CalB subnucleus, but spared other compartments of the SCN, lost their locomotor activity rhythm. Animals with partial bilateral SCN lesions sparing part ...

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“…In the which contains the mammalian biological clock (1, 2), exhibCa 2 +-free medium, MgCI 2 was substituted for CaCd 2 , and 0.1 its a circadian rhythm where peak activity occurs at the mM 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic middle of the light phase, even in a surgically isolated acid, a specific Ca 2 + chelator, was added. Blockade of hypothalamus (3) or in vitro brain slice (4)(5)(6)(7). Because these N-methyl-D-aspartic acid (NMDA), non-NMDA, and yamidata are derived from neuronal populations, they also imply nobutyric acid type A (GABAA) receptors was achieved by that the electrical activity generating the pacemaker is synbath application of 100 /AM DL-2-amino-5-phosphonopenchronized.…”

Section: Solutions For the Circadian Rhythm Experiments (See Figmentioning

confidence: 99%

“…in vitro studies (3)(4)(5)(6)(7). The correlation between the two hourly Whole-Cell Patch-Clamp Recordings.…”

Section: Solutions For the Circadian Rhythm Experiments (See Figmentioning

confidence: 99%

Cellular Neurophysiology of the Rat Suprachiasmatic Nucleus: Electrical Properties, Neurotransmission, and Mechanisms of Synchronization

Dudek¹

1994

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Interaction of colocalized neuropeptides: functional significance in the circadian timing system

Cited by 202 publications

References 23 publications

Peptide Expression in GABAergic Neurons in Rat Suprachiasmatic Nucleus in Comparison with Other Forebrain Structures: A Double Labeling In Situ Hybridization Study

Peptide Expression in GABAergic Neurons in Rat Suprachiasmatic Nucleus in Comparison with Other Forebrain Structures: A Double Labeling In Situ Hybridization Study

Calbindin-D28K cells selectively contact intra-SCN neurons

Cellular Neurophysiology of the Rat Suprachiasmatic Nucleus: Electrical Properties, Neurotransmission, and Mechanisms of Synchronization

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