Interaction of colchicine with phosphatidylcholine membranes

Altstiel, Lawrence D.; Landsberger, Frank R.

doi:10.1038/269070a0

Cited by 20 publications

(5 citation statements)

References 22 publications

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“…Besides a specific binding of colchicine to tubulin, colchicine als0 binds to membrane fractions (Wunderlich et al, 1973;Stadler and Franke, 1974;Wilson et al, 1974;Altstiel and Landsberger, 1977). In addition, arecent study demonstrated a direct and specific binding of tubulin to phospholipid bilayers (Caron and Berlin, 1974).…”

Section: Discussionmentioning

confidence: 99%

An electron microscopic radioautographic study of collagen secretion in periodontal ligament fibroblasts of the mouse: I. Normal fibroblasts

Cho

Garant

1981

Anat. Rec.

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Analysis of electron microscopic radioautographs revealed a maximum labeling with 3H-proline of rough endoplasmic reticulum (RER) at 3 minutes, Golgi saccules 1 and 2 at 10 minutes, Golgi saccules type 3 at 20 minutes, and presecretory and secretory granules at 30 minutes. Labeling of the extra-cellular collagen matrix occurred at 30 minutes and increased with time. These observations suggest that pro-a-chains of collagen in periodontal ligament fibroblasts are synthesized in the RER and transported to the Golgi apparatus within 10 minutes. These chains then undergo parallel alignment in Golgi saccules type 2 and form segment-long-spacing-like crystallites in Golgi saccules type 3 between 10 and 20 minutes. The peak labeling of presecretory granules and mature secretory granules in small amounts at 30 minutes and the rapid increase in labeling of extracellular collagen matrix which begins at 30 minutes, indicates that the formation of secretory granules requires approximately 30 minutes and that a rapid system of secretory granule translocation exists in periodontal ligament fibroblasts. This evidence further supports the previously published morphologic evidence for a microtubule-dependent system of collagen secretion in periodontal ligament fibroblasts (Cho and Garant, 1981b).

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Section: Discussionmentioning

confidence: 99%

An electron microscopic radioautographic study of collagen secretion in periodontal ligament fibroblasts of the mouse: I. Normal fibroblasts

Cho

Garant

1981

Anat. Rec.

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“…One alternative hypothesis regarding the inhibition of secretion by both local anesthetics and colchicine is that both may act on the membrane and that the microtubular system is not involved in the inhibition of secretion. Colchicine can influence membrane phenomena such as nucleotide transport (23), and appears to interact with membrane protein (1). If inhibition of secretion were unrelated to colchicine's actions on the microtubular system, then possibly both colchicine and the local anesthetics cause inhibition by effects on the cell and/or Golgi membranes that might, for example, prevent fusion of secretory granules with the plasma membrane.…”

Section: Discussionmentioning

confidence: 99%

Local anesthetic-induced inhibition of collagen secretion in cultured cells under conditions where microtubules are not depolymerized by these agents.

Eichhorn¹,

Peterkofsky²

1979

The Journal of Cell Biology

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Tertiary amine local anesthetics previously have been shown to influence some microtubule-dependent cellular functions. Since several cell secretion processes, including secretion of collagen, have been shown to be inhibited by microtubuledisrupting drugs such as colchicine, we determined whether local anesthetics affect collagen secretion. Six local anesthetics inhibited collagen and non-collagen protein secretion (up to 98%) into the extracellular medium of 3T3 cells and human fibroblasts, an effect apparently independent of influences on proline transport and total protein synthesis.A combination of colchicine and cytochalasin B did not duplicate the effects of local anesthetics. The effects of subsaturating concentrations of colchicine and procaine on secretion were additive, suggesting that both drugs act on the secretory pathway at the level of microtubules, but other effects of the two types of drugs were strikingly different.In comparing the mechanisms of action of colchicine and local anesthetics, it was seen that, in contrast to colchicine, radioactive procaine and lidocaine were slowly transported into 3T3 cells, did not bind to the tubulin-containing TCAinsoluble fraction, and did not bind to purified tubulin in vitro. The fraction of cellular tubulin present as microtubules (47% in normal cells) was determined by measuring tubulin in stabilized, sedimentable microtubules compared to total tubulin, using a [ZH]colchicine binding assay. Pretreatment of cells in the cold or with colchicine led to depolymerization of microtubules, but pretreatment with five local anesthetics tested did not. Therefore, in contrast to colchicine, local anesthetics in concentrations that inhibit secretion do not directly interact with or depolymerize microtubules. These drugs, however, do affect a microtubuledependent process and may do so by detaching the microtubular system from the cell membrane.

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“…must be developed to provide an adequate experimental description of the potential profile and concentration of counterions for x <2 nm. Altstiel and Landsberger (1987) have used an electron spin resonance technique to estimate the potential as a function of distance from a charged phospholipid bilayer. pH indicator dyes confined to an interface have long been used to measure the surface pH and deduce the surface potential (Hartley & Roe, 1940;Fromherz, 1973;Fromherz & Masters, 1974;Vaz et al, 1978).…”

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confidence: 99%

Fluorescent probes of electrostatic potential 1 nm from the membrane surface

Winiski¹,

Eisenberg²,

Langner³

et al. 1988

Biochemistry

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We measured the electrostatic potential 1 nm from the surface of charged phospholipid bilayer membranes to test the predictions of the Gouy-Chapman theory. Fluorescent probes (anthraniloyl, 5-(dimethylamino)naphthalene-1-sulfonyl, Lucifer yellow) were attached covalently to the sialic acid residue of the ganglioside galactosyl-N-acetylgalactosaminyl(N-acetylneuraminyl)galactosylglucosylc eramide (GM1). These fluorescent gangliosides were incorporated into neutral [phosphatidylcholine (PC)] or charged [phosphatidylserine (PS)] phospholipid bilayers, and the fluorescence was quenched with the cations thallium and 4-amino-2,2,6,6-tetramethylpiperidine-1-oxyl (tempamine). We calculated the electrostatic potential at the chromophore from the quenching ratio using the Boltzmann relation: the average potential was -30 mV for PS bilayers in 0.1 M NaNO3. We assume the chromophore is 1 nm from the surface because X-ray diffraction measurements demonstrate that the sialic acid residue of GM1 is 1 nm from the surface of a PC/GM1 bilayer [McDaniel, R. V., & McIntosh, T. J. (1986) Biophys. J. 49, 94-96]. We also used thallium and tempamine to quench the fluorescence of chromophores located at the surface of the PS membranes; in 0.1 M NaNO3 the average surface potential was -80 mV, which agrees with other measurements. The Gouy-Chapman theory predicts that the potential 1 nm from a membrane with a surface potential of -80 mV is -24 mV; this prediction agrees qualitatively with the experimental results obtained with fluorescent gangliosides.

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Interaction of colchicine with phosphatidylcholine membranes

Cited by 20 publications

References 22 publications

An electron microscopic radioautographic study of collagen secretion in periodontal ligament fibroblasts of the mouse: I. Normal fibroblasts

An electron microscopic radioautographic study of collagen secretion in periodontal ligament fibroblasts of the mouse: I. Normal fibroblasts

Local anesthetic-induced inhibition of collagen secretion in cultured cells under conditions where microtubules are not depolymerized by these agents.

Fluorescent probes of electrostatic potential 1 nm from the membrane surface

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