Interaction of Coenzyme Q10 with Dipalmitoylphosphatidylcholine Liposomes

Abstract

The goal of this study to investigate the influence of antioxidant as coenzyme Q10 on structural changes of model lipid membranes as DPPC by using Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC). The results obtained indicate that the greater effect of CoQ 10 upon the incorporation into negatively charged liposomes in comparison with the other liposome formulations. The incorporated CoQ10 is probably associated with the lipid bilayers, interacted to a large extent with them, and perturbed them which results in the strong broadening and shift to lower temperature 94 C of the major characteristic endothermic peak of pure DPPC that exists at 105 C. The FT-IR spectra of samples showed that Incorporation of CoQ10 into neutrally, positively and negatively charged DPPC liposomes showed significant change in the frequency of the antisymmetric CH2 stretching bands in the acyl chain implying that CoQ10 create a conformational disorder within the acyl chains of phospholipids. In other words, it had significant effect on the order of the membrane.