Interaction of cells on chargeable functional group gradient surfaces

Lee, Jin Ho; Lee, Jin Whan; Khang, Gilson; Lee, Hai Bang

doi:10.1016/s0142-9612(96)00128-7

Cited by 238 publications

(183 citation statements)

References 24 publications

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“…This behavior was attributed more to the hydrophilicity than the effect of the functional group. 79,81 The reason for relatively insignificant effects of functional group density on in vivo tissue responses, as observed in the present study, is not clear at this moment. It is possible that once a threshold density of a particular functional group is made available, further increases in these groups are relatively ineffective in that an essentially similar initial biomolecule surface-interaction, ultimately responsible for the capsule formation, cell infiltration, etc., dominates under in vivo conditions, independent of functional group concentrations, independent on the surface chemistry.…”

Section: Discussioncontrasting

confidence: 68%

“…This observation can be correlated with previous studies which have linked the negatively charged surface functionality inhibiting the migration of negatively charged cells towards the functionalized surface. 43,68,[78][79][80] Rather unexpectedly, we find that the density of surface functionality has little or no influence on tissuereactions to microsphere implants. These results contradict many in vitro observations which found cell adhesion to be directly correlated to the density of functionalization.…”

Section: Discussionmentioning

confidence: 61%

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Species and Density of Implant Surface Chemistry Affect the Extent of Foreign Body Reactions

et al. 2008

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Implant-associated fibrotic capsule formation presents a major challenge for the development of long term drug release microspheres and implantable sensors. Since material properties have been shown to affect in vitro cellular responses and also to influence short term in vivo tissue responses, we have thus assumed that the type and density of surface chemical groups would affect the degree of tissue responses to microsphere implants. To test this hypothesis, polypropylene particles with different surface densities of -OH and -COOH groups, along with the polypropylene control (-CH 2 groups) were utilized. The influence of functional groups and their surface densities on tissue reactions were analyzed using a mice subcutaneous implantation model. Our comparative studies included determination and correlation of the extents of fibrotic capsule formation, cell infiltration into the particles and recruitment of CD11b+ inflammatory cells for all of the substrates employed. We have observed major differences among microspheres coated with different surface functionalities. Surfaces with -OH surface groups trigger the strongest responses while -COOH rich surfaces prompt the least tissue reactions. However, variation of the surface density of either functional group has a relatively minor influence on the extent of fibrotic tissue reactions. The present results show that surface functionality can be used as a powerful tool to alter implant-associated fibrotic reactions and, potentially, to improve the efficacy and function of drug delivery microspheres, implantable sensors and tissue engineering scaffolds.

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Section: Discussioncontrasting

confidence: 68%

Section: Discussionmentioning

confidence: 61%

Species and Density of Implant Surface Chemistry Affect the Extent of Foreign Body Reactions

et al. 2008

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“…The physicochemical properties of different materials could modulate cell morphology and behavior. Signaling from polymers that drive the cell growth pattern is complex and may originate from dipole and electric charge interaction forces, hydrogen bonds, electrostatic forces, hydrophilicity/hydrophobicity or surface free energy, and roughness and rigidity, surface tension, and substrate topography (18)(19)(20). The morphology of Vero cells on pure PLLA scaffolds observed here was similar to previous results obtained by our group (7,8,21), which showed that round or spreading cells attached on PLLA surface by thin cytoplasmatic filaments.…”

Section: Discussionmentioning

confidence: 99%

Use of blends of bioabsorbable poly(L-lactic acid)/poly(hydroxybutyrate- co-hydroxyvalerate) as surfaces for Vero cell culture

Santos

Ferreira

Duek

et al. 2005

Braz J Med Biol Res

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Vero cells, a cell line established from the kidney of the African green monkey (Cercopithecus aethiops), were cultured in F-10 Ham medium supplemented with 10% fetal calf serum at 37°C on membranes of poly(L-lactic acid) (PLLA), poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and their blends in different proportions (100/0, 60/40, 50/50, 40/60, and 0/100). The present study evaluated morphology of cells grown on different polymeric substrates after 24 h of culture by scanning electron microscopy. Cell adhesion was also analyzed after 2 h of inoculation. For cell growth evaluation, the cells were maintained in culture for 48, 120, 240, and 360 h. For cytochemical study, the cells were cultured for 120 or 240 h, fixed, processed for histological analysis, and stained with Toluidine blue, pH 4.0, and Xylidine ponceau, pH 2.5. Our results showed that cell adhesion was better when 60/40 and 50/50 blends were used although cells were able to grow and proliferate on all blends tested. When using PLLA/PHBV (50/50) slightly flattened cells were observed on porous and smooth areas. PLLA/PHBV (40/60) blends presented flattened cells on smooth areas. PLLA/PHBV (0/100), which presented no pores, also supported spreading cells interconnected by thin filaments. Histological sections showed that cells grew as a confluent monolayer on different substrates. Cytochemical analysis showed basophilic cells, indicating a large amount of RNA and proteins. Hence, we detected changes in cell morphology induced by alterations in blend proportions. This suggests that the cells changed their differentiation pattern when on various PLLA/PHBV blend surfaces. Correspondence

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“…Its importance in the design of implantable scaffolds was first reported by Weiss in 1960, 16 later confirmed by many others. 5,[17][18][19][20][21][22] Since cells effectively adhered onto polymer surfaces with moderate wettability as demonstrated by water contact angles of 40-70 degress, [23][24][25][26][27] it is clear that the wettability of a biomaterial surface plays a critical role in cell attachment and proliferation. [28][29][30] In addition, it has been shown that most human bone marrow stromal cells attached to hydrophobic (HP) surfaces had smaller surface contact areas, resulting in a more rounded cell morphology.…”

Section: Introductionmentioning

confidence: 99%

Carbon nanotubes impregnated with subventricular zone neural progenitor cells promotes recovery from stroke

Moon¹,

Kim²,

Bokara³

et al. 2012

IJN

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The present in vivo study was conducted to evaluate whether hydrophilic (HL) or hydrophobic (HP) carbon nanotubes (CNTs) impregnated with subventricular zone neural progenitor cells (SVZ NPCs) could repair damaged neural tissue following stroke. For this purpose, stroke damaged rats were transplanted with HL CNT-SVZ NPCs, HP CNT-SVZ NPCs, or SVZ NPCs alone for 1, 3, 5, and 8 weeks. Results showed that the HP CNT-SVZ NPC transplants improved rat behavior and reduced infarct cyst volume and infarct cyst area compared with the experimental control and the HL CNT-SVZ NPC and SVZ NPCs alone groups. The transplantation groups showed an increase in the expression of nestin (cell stemness marker) and proliferation which was evident with the increased number of doublecortin and bromodeoxyuridine double-stained immunopositive cells around the lesion site. But, these effects were more prominent in the HP CNT-SVZ NPC group compared with the other transplantation groups. The HP CNT-SVZ NPC and HL CNT-SVZ NPC transplants increased the number of microtubule-associated protein 2 (marker for neurons) and decreased the number of glial fibrillary acidic protein (marker for astroglial cells) positive cells within the injury epicenter. The majority of the transplanted HP CNT-SVZ NPCs collectively broadened around the ischemic injured region and the SVZ NPCs differentiated into mature neurons, attained the synapse morphology (TUJ1, synaptophysin), and decreased microglial activation (CD11b/c ). For these reasons, this study provided the first evidence that CNTs can improve stem cell differentiation to heal stroke damage and, thus, deserve further attention.

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Interaction of cells on chargeable functional group gradient surfaces

Cited by 238 publications

References 24 publications

Species and Density of Implant Surface Chemistry Affect the Extent of Foreign Body Reactions

Species and Density of Implant Surface Chemistry Affect the Extent of Foreign Body Reactions

Use of blends of bioabsorbable poly(L-lactic acid)/poly(hydroxybutyrate- co-hydroxyvalerate) as surfaces for Vero cell culture

Carbon nanotubes impregnated with subventricular zone neural progenitor cells promotes recovery from stroke

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