Interaction of CD99 and its ligand upregulates IL-6 and TNF-α upon T cell activation

Takheaw, Nuchjira; Earwong, Papawadee; Laopajon, Witida; Kasinrerk, Watchara

doi:10.1371/journal.pone.0217393

Cited by 23 publications

(24 citation statements)

References 56 publications

(41 reference statements)

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“…Ligation of CD99 on the activated T cells by a CD99 recombinant protein, increased production of proinflammatory cytokines, including interleukin-6 (IL-6) and TNF-α. Engagement of CD99 on monocytes and natural killer cells also resulted in the increased production of IL-6 and TNF-α (Takheaw et al 2019). Waclavicek et al demonstrated resting T cells in response to CD99 stimulation and T cell receptor (TCR) activation, proliferate and produce IFN-γ and TNF-α, (Waclavicek 1998).…”

Section: Cluster Of Differentiation 99 (Cd99)mentioning

confidence: 99%

Escape from X chromosome inactivation and female bias of autoimmune diseases

Mousavi

Mahmoudi

Ghotloo

2020

Mol Med

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Generally, autoimmune diseases are more prevalent in females than males. Various predisposing factors, including female sex hormones, X chromosome genes, and the microbiome have been implicated in the female bias of autoimmune diseases. During embryogenesis, one of the X chromosomes in the females is transcriptionally inactivated, in a process called X chromosome inactivation (XCI). This equalizes the impact of two X chromosomes in the females. However, some genes escape from XCI, providing a basis for the dual expression dosage of the given gene in the females. In the present review, the contribution of the escape genes to the female bias of autoimmune diseases will be discussed.

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Section: Cluster Of Differentiation 99 (Cd99)mentioning

confidence: 99%

Escape from X chromosome inactivation and female bias of autoimmune diseases

Mousavi

Mahmoudi

Ghotloo

2020

Mol Med

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“…The β isoform is expressed at higher levels on Natural Killer (NK) cells, B cells, T cells, dendritic cells, granulocytes and macrophages, while the α-isoform is expressed at very low levels on NK cells [27]. Takheaw et al showed that the interaction of a recombinant CD99 with its ligand on immune cells upregulated the production of pro-inflammatory cytokines [28].…”

Section: Introductionmentioning

confidence: 99%

Association of Maternal Regulatory Single Nucleotide Polymorphic CD99 Genotype with Preeclampsia in Pregnancies Carrying Male Fetuses in Ethiopian Women

Kelemu

Erlandsson

Seifu

et al. 2020

IJMS

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Preeclampsia (PE) is a human specific syndrome with unknown etiology causing maternal and fetal morbidities and mortalities. In PE, maternal inflammatory responses are more exaggerated if the fetus is male than female. Other pregnancy complications such as spontaneous abortions are also more common if the fetus is male. Recent transcriptome findings showed an increased expression of CD99 in erythroid cells from male cord blood in PE. The single nucleotide polymorphism (SNP) rs311103, located in a GATA-binding site in a regulatory region on the X/Y chromosomes, governs a coordinated expression of the Xg blood group members CD99 and Xga in hematopoietic cells in a sex-dependent fashion. The rs311103C disrupts the GATA-binding site, resulting in decreased CD99 expression. We aimed to investigate the association between PE and the allele frequency of rs311103 in pregnancies in a fetal sex-dependent fashion. In a case-controlled study, we included 241 pregnant women, i.e., 105 PE cases and 136 normotensive controls. A SNP allelic discrimination analysis was performed on DNA from maternal venous blood and fetal cord blood by qPCR. A statistically significant association was observed between rs311103 allele frequency and PE in mothers carrying male fetuses. Therefore, the rs311103 genotype may play a role in the pathogenesis of PE in a fetal sex-specific manner.

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Section: Introductionmentioning

confidence: 99%

“…Recently, we demonstrated that putative CD99 ligands were expressed on the surfaces of monocytes, NK cells and dendritic cells, but not on those of B and T cells [ 14 ]. Recombinant CD99 protein enhanced the upregulation of IL-6 and TNF-α expression by T cells, monocytes and NK cells [ 14 ]. The presence of undefined CD99 ligands on leukocyte surfaces as well as regulation of the immune response via interaction between CD99 and its ligands has been suggested [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…Recombinant CD99 protein enhanced the upregulation of IL-6 and TNF-α expression by T cells, monocytes and NK cells [ 14 ]. The presence of undefined CD99 ligands on leukocyte surfaces as well as regulation of the immune response via interaction between CD99 and its ligands has been suggested [ 14 ]. Thus, identification of CD99 ligands has become necessary in order to broaden the understanding of CD99 functions involved in immunoregulation.…”

Section: Introductionmentioning

confidence: 99%

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The presence of membrane bound CD99 ligands on leukocyte surface

et al. 2020

Self Cite

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Objective CD99, a leukocyte surface molecule, reportedly plays an important role in several cellular processes. However, the role of CD99 in T cell regulation remains unclear, as the CD99 ligand associated with T-cell regulation has not yet been identified. Our previous study showed that recombinant CD99 bound to CD99 ligands was expressed on monocytes, NK cells and dendritic cells. This interaction regulates the expression of IL-6 and TNF-α in CD3 + T cells following T cell activation. In the present study, we confirmed the presence of CD99 ligands in immune cells. Results A recombinant CD99-human IgG fusion protein, CD99HIgG, was produced and used to search for CD99 ligand expression in various hematopoietic cell lines. Among several cell lines, THP-1 monocytic cell line showed strong positive reaction for CD99HIgG, and CD99 and CD99 ligand complexes were pulled-down using a DTSSP cross-linker. The study demonstrated the presence of the membrane bound CD99 ligand, and CD99 ligand candidates were identified via LC–MS/MS. These results may be useful to further identify the CD99 ligands, and to fully comprehend the role of CD99 in immunoregulation.

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Interaction of CD99 and its ligand upregulates IL-6 and TNF-α upon T cell activation

Cited by 23 publications

References 56 publications

Escape from X chromosome inactivation and female bias of autoimmune diseases

Escape from X chromosome inactivation and female bias of autoimmune diseases

Association of Maternal Regulatory Single Nucleotide Polymorphic CD99 Genotype with Preeclampsia in Pregnancies Carrying Male Fetuses in Ethiopian Women

The presence of membrane bound CD99 ligands on leukocyte surface

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