Interaction of calmodulin with iodothyronines: effect of iodothyronines on the calmodulin activation of cyclic AMP phosphodiesterase

Rao, Govind S.; Lemoch, H; Cronrath, Christoph M.

doi:10.1016/0303-7207(87)90190-0

Cited by 7 publications

(5 citation statements)

References 20 publications

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“…This, and the fact that Gal evoked a weak contraction under conditions of extracellular Ca 2 de®ciency or absence, proves that Ca 2 release from the intracellular stores contributes sig-ni®cantly to the smooth muscle response to Gal (Fontaine & Lebrun 1989;Korolkiewicz et al 1997). Tri¯uoroperazine inhibited the effect of Gal which is consistent with calmodulin being intimately involved in contraction of intact smooth muscle strips (Rao 1987).…”

Section: Discussionsupporting

confidence: 61%

Mechanisms of Galanin-induced Contraction of Isolated Rat Gastric Fundus Strips†

Korolkiewicz¹,

Konstański²,

Rekowski³

et al. 2000

Pharmacy and Pharmacology Communications

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Galanin (Gal) constricts rat gastric fundus by acting on receptors located in the cell membrane. We compared the role of intracellular Ca 2 release with extracellular Ca 2 in¯ux in Gal-stimulated contraction of isolated gastric smooth muscle strips. We also tested if phospholipase C (PLC) or protein kinase C (PKC) participate in the signal transduction cascade.Concentration±contraction curves were constructed non-cumulatively in the presence of atropine, hexamethonium, guanethidine and tetrodotoxin. The half-maximum effective concentration (EC50) of Gal was 21Á62 nM and Hill's coef®cient was 1Á02. The effects of Gal were decreased by diltiazem, Ca 2 -de®ciency in the buffer, Ca 2 removal from the extracellular medium or quercetin. Depletion of intracellular Ca 2 -stores, ryanodine and 3,4,5-trimethoxybenzoic acid 8-(diethylamino)-octyl ester diminished the contractile effect of Gal concentration-dependently. Tri¯uoroperazine and phosphatidylinositol-speci®c phospholipase C (PI-PLC) inhibitors, neomycin and U-73122, attenuated the gastric fundus response to Gal, whereas phosphatidylcholine-speci®c phospholipase C (PC-PLC) and phospholipase D (PLD) blockers, D609 and propranolol, were ineffective. The inhibitors of PKC or myosin light chain kinase, calphostin C, chelerythrine, ML-7 and ML-9, lowered the myogenic activity of Gal.Our data con®rmed that the stimulation of Gal receptors in gastric fundus is coupled to Ca 2 in¯ux through voltage-dependent channels and intracellular Ca 2 release from ryanodine-and inositol 1,4,5-triphosphate-sensitive stores. Enzymes such as PI-PLC and PKC, but not PC-PLC or PLD, play a role in the signal transduction cascade. Calmodulin and myosin light chain kinase lie downstream of the increases in intracellular Ca 2 concentration evoked by Gal.Porcine galanin (Gal) is a 29 amino acid peptide isolated for the ®rst time by Tatemoto et al (1983). It is implicated in the modulation of cognition, memory, feeding, nociception, sexual behaviour and spinal re¯exes (Ro Èkaeus 1987;Bartfai 1995). Its action is exerted by the stimulation of three high af®nity G protein-coupled membrane receptors and the subsequent activation of several intracellular signalling pathways (

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Section: Discussionsupporting

confidence: 61%

Mechanisms of Galanin-induced Contraction of Isolated Rat Gastric Fundus Strips†

Korolkiewicz¹,

Konstański²,

Rekowski³

et al. 2000

Pharmacy and Pharmacology Communications

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“…The mechanism whereby Ta might inhibit PDE activity in fish is not known. However, in porcine brain, Ts inhibits Ca2+-calmodulin activation of CAMP-dependent PDE (Rao et al, 1987). TH also increases cell Ca2+ uptake (Segal and Ingbar, 1986).…”

Section: Ovarian Development and Vitellogenesismentioning

confidence: 99%

Interrelationships between thyroidal and reproductive endocrine systems in fish

Cyr

Eales

1996

Rev Fish Biol Fisheries

186

127

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“…A reasonable interpretation of these data is that the scope for T 3 to enhance cAMP action by inhibiting PDE activity is swamped in the presence of theophylline which itself effectively blocks PDE. Support for T 3 inhibition of PDE comes from recent mammalian studies where iodothyronine interaction with calmodulin decreases PDE activity in the brain (Rao et al 1987). Smoake et al (1988 have observed that the activity of certain isoforms of PDE in liver cells varies with thyroid status and that a low molecular weight form may in fact be regulated by T 3.…”

Section: Resultsmentioning

confidence: 99%

“…While similar effects on other tissues from other animals remain to be demonstrated it is possible that T 3 action upon trout ovarian follicles is mediated by this pathway. It has been shown in rats that physiological elevations in intracellular Ca 2 § can decrease PDE activity in liver tissue and augment intracellular concentrations of cAMP (Rao et al 1987).…”

Section: Resultsmentioning

confidence: 99%

T3 enhancement ofin vitro estradiol-17β secretion by oocytes of rainbow trout,Salmo gairdneri, is dependent on cAMP

Cyr

Eales

1989

Fish Physiol Biochem

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The cellular mechanism of action of 3,5,3'-triiodo-L-thyronine (T3) in enhancing SG-G100 gonadotropin-induced ovarian secretion of 17β-estradiol (E2) was studiedin vitro using oocyte follicular preparations of rainbow trout. The dependence of the T3 stimulatory action on the level of intracellular 3',5'-cyclic adenosine monophosphate (cAMP) was shown in experiments in which forskolin or dibutyryl cAMP enhanced E2 secretion. In the presence of partially purified salmon gonadotropin (SG-G100), T3 stimulation of E2 secretion was prevented by theophylline, suggesting that T3 may exert part of its stimulatory action by inhibiting phosphodiesterase.

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Interaction of calmodulin with iodothyronines: effect of iodothyronines on the calmodulin activation of cyclic AMP phosphodiesterase

Cited by 7 publications

References 20 publications

Mechanisms of Galanin-induced Contraction of Isolated Rat Gastric Fundus Strips†

Mechanisms of Galanin-induced Contraction of Isolated Rat Gastric Fundus Strips†

Interrelationships between thyroidal and reproductive endocrine systems in fish

T3 enhancement ofin vitro estradiol-17β secretion by oocytes of rainbow trout,Salmo gairdneri, is dependent on cAMP

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