Interaction of Bovine Serum Albumin with Cationic Single Chain+Nonionic and Cationic Gemini+Nonionic Binary Surfactant Mixtures

Abstract

The interaction of bovine serum albumin (BSA) with cetyltrimethylammonium bromide (CTAB), C(16)C(4)C(16)Br(2), Brij58, and their binary mixtures has been studied using tensiometry, spectrofluorometry, and circular dichroism at physiological pH and 25 degrees C. The tensiometric profiles of CTAB and C(16)C(4)C(16)Br(2) in the presence of BSA exhibit a single break at a lower surfactant concentration termed as C(1) (concentration corresponding to saturation of the interface) compared to their critical micelle concentration (CMC) in the buffered solution. However, for Brij58, CTAB+Brij58, and C(16)C(4)C(16)Br(2)+Brij58, two breaks were observed, first at the critical aggregation concentration (CAC), corresponding to onset of interaction with BSA and the second at C(1) corresponding to saturation of the interface. The interaction of CTAB+Brij58 and C(16)C(4)C(16)Br(2)+Brij58 mixtures with the BSA solution is discussed in terms of competition between surfactant-surfactant and surfactant-BSA interactions. CTAB+Brij58 and C(16)C(4)C(16)Br(2)+Brij58 mixtures show nonideality with respect to mixed micelle formation, which is reflected in their interaction with the BSA. The interaction of CTAB+Brij58 with BSA decreases with increase in the mole fraction of CTAB in the mixture, whereas in C(16)C(4)C(16)Br(2)+Brij58 the reverse is the case. The results of the present study may prove fruitful in optimizing the properties of surfactant-protein mixtures relevant for many formulations.

Investigation of bovine serum albumin-surfactant aggregation and its physicochemical characteristics

“…Tripledistilled water (conductance = 1 × 10 −6 mho) was used within seven days of its preparation. Freshly prepared solutions of BSA and surfactants in phosphate buffer of pH 7.4 with triple distilled water [27] were used for each measurement. Different percentages of BSA solution were prepared by dissolving BSA in grams per 100 mL of the solution.…”

“…Although at 278 nm, both the Trp and Tyr residues are excited, the fluorescence behavior of BSA is mainly due to Trp, and an emission peak appeared at 340 nm [35]. The wavelength of emission maximum and the fluorescence intensity at 340 nm are sensitive to the protein conformation, and hence the intrinsic fluorescence intensity on excitation at 278 nm can be used to examine the protein-surfactant interactions [27]. Fig.…”

Molecular array behavior and synergistic effect of sodium alcohol ether sulphate and carboxyl betaine/sulfobetaine in foam film under high salt conditions

“…Most of the literatures about the synergistic effect of the mixed surfactants focused on the mixture of nonionic/ionic surfactants and amphoteric/ionic surfactants [12][13][14][15][16][17][18][19]. It has been reported that the co-adsorption of non-ionic/ionic surfactants or amphoteric/ionic surfactants on the gas/water or oil/water interfaces could improve the interfacial activity significantly by reducing the electrical repulsion between the charged head groups and increasing the interfacial density of the adsorbed molecules [20,21].…”

Environment-friendly ester bonded gemini surfactant: Mixed micellization of 14-E2-14 with ionic and nonionic conventional surfactants

“…Owing to their unusual properties (such as high surface activity, much lower cmc, higher solubilization capacity, low Krafft temperature, good wetting, detergency and emulsifying properties), the geminis have been considered the next generation surfactants [1][2][3][4][5][6][7]. Cationic geminis, in particular, have been shown to possess high antifungal, antibacterial and antimicrobial activities [8][9][10][11] and have attracted considerable attention towards their interaction with biologically important ligands [12][13][14][15][16]. Surfactants interact with the membranes causing cell lysis.…”

“…Surfactants interact with the membranes causing cell lysis. Cell lysis by the surfactants has fundamental and practical importance mainly in drug targeting or in pharmacology [14][15][16]. Despite very encouraging results of the above interaction studies, the gemini surfactants invariably been used are of m-s-m type (m and s are the number of carbon atoms in the hydrophobic tail and the spacer parts, respectively).…”