Interaction of bovine serum albumin with nonionic surfactant Tween 80 in aqueous solutions: Complexation and association

Zadymova, N. M.; Yampol’skaya, G. P.; Filatova, L. Yu.

doi:10.1134/s1061933x06020074

Cited by 38 publications

(26 citation statements)

References 24 publications

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“…In the conditions of the latter state there can be the formation of duplex structures by the second layer, accompanied by the formation of micelles with their subsequent irreversible transition into the aqueous phase (Zadymova 2006). In this paper, a preliminary assessment of the effectiveness of extracting action of additives of various alcohols (isobutyl, isoamyl, isopropyl, hexyl, ethyl, methyl etc.)…”

Section: Resultsmentioning

confidence: 99%

Influence of the isolation method of the soapstockfatty component on its characteristics

Prokof’ev¹,

Poyarkova²,

Bykov³

et al. 2015

Res. Agric. Eng.

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Prokof'ev Y.I., Poyarkova T.N., Bykov A.R., Shestakov A.S., Kuznetsov V.A., Androsova O.G. (2015): Influence of the isolation method of the soapstock fatty component on its characteristics. Res. Agr. Eng., 61: 111-115.From soapstock, which is a waste product of sunflower oil production, the fatty component was isolated using isopropyl alcohol, toluene, fusel oil and a mixture of common salt and nonionic surfactants. With the help of the Langmuir film balance the molecular areas of isolated fats were found and the degree of hydrophobization in comparison with the original soapstock was evaluated. It was found that the strongest compression of the monolayer is observed when using fusel oil. The surface active properties of emulsifiers, synthesized by alkaline hydrolysis method on the basis of the obtained fat extracts and original soapstock, were studied. These results are consistent with measurements on the Langmuir balance.

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Section: Resultsmentioning

confidence: 99%

Influence of the isolation method of the soapstockfatty component on its characteristics

Prokof’ev¹,

Poyarkova²,

Bykov³

et al. 2015

Res. Agric. Eng.

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“…A possible explanation is that introduction of the electrolyte has a destructive effect on the hydrophobic complex ASF-NSF formed, which probably affects the structure of water. This phenomenon can be understood on the basis of the data of [14,15]. If the concentration of ASFs exceeds that of NSFs, 1 : 1 ASF-NSF are predominantly formed.…”

Section: Methodsmentioning

confidence: 98%

Effect of inorganic electrolytes and nonionogenic surfactants on the stability of soap stock emulsion

2012

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Modes in which the fat component of the soap stock emulsion is extracted under heating and introduction of various nonionogenic surfactants and electrolytes were studied. The compression isotherms of the soap stock and its mixtures with OP-7 and NaCl were examined by the Langmuir method, with 2D pressure recorded. Recommendations were developed for separation of the soap stock emulsion for recovery of the fat component from wastes produced in manufacture of vegetable oils.

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“…The surfactant interacts with the protein predominantly via the polyoxyethylene groups of their polar heads through hydrogen bonds and van der Waals interactions. In addition, polysorbate 80 binds to non-specific surface regions on albumin, selfassembling in clusters over the protein surface as micelles through cooperative hydrophobic interactions with other surfactant molecules in solution, thus increasing the solubility of the surfactant: protein complex (Zadymova et al, 2006). These properties of polysorbate 80 were exploited to prepare the stable HSA NPs of Iro with enhanced drug entrapment and loading.…”

Section: Preparation Of Iro-hsa-npsmentioning

confidence: 99%

Rational design of polysorbate 80 stabilized human serum albumin nanoparticles tailored for high drug loading and entrapment of irinotecan

Taneja

Singh

2018

International Journal of Pharmaceutics

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Human serum albumin (HSA) nanoparticles are considered to be versatile carrier of anticancer agents in efficiently delivering the drug to the tumor site without causing any toxicity. The aim of the study was to develop stable HSA nanoparticles (NPs) of drug irinotecan (Iro) having slightly water solubility and moderate HSA binding. A novel strategy of employing a hydrophilic non-ionic surfactant polysorbate 80 which forms protein-polysorbate 80 complex with increased affinity and improvement in Iro-HSA binding has been used to maximize the loading and entrapment efficiency of Iro in HSA-NPs. Bespoke nanoparticles with entrapment efficiency (79.09%) and drug loading of 9.62% could be achieved with spherical shape and particle size of 77.38 nm, 0.290 polydispersity index and -23.7 mv Zeta potential. The drug entrapment in nanoparticles was confirmed by Differential Scanning Calorimeter, Fourier Transformation Infrared Spectroscopy and Fluorescence Spectroscopy. In vitro release of Iro from NPs showed biphasic-release with initial burst followed by prolonged release upto 24 h. The short-term stability investigation of nanodispersion showed no significant changes in physicochemical properties of NPs. Long-term studies on freeze dried Iro-HSA-NPs indicated good stability of NPs up to 12 months. This is the first report for efficient fabrication of Iro delivery system based on HSA nanoparticles.

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Interaction of bovine serum albumin with nonionic surfactant Tween 80 in aqueous solutions: Complexation and association

Cited by 38 publications

References 24 publications

Influence of the isolation method of the soapstockfatty component on its characteristics

Influence of the isolation method of the soapstockfatty component on its characteristics

Effect of inorganic electrolytes and nonionogenic surfactants on the stability of soap stock emulsion

Rational design of polysorbate 80 stabilized human serum albumin nanoparticles tailored for high drug loading and entrapment of irinotecan

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