Interaction of bone marrow stromal cells with lymphoblasts and effects of predinsolone on cytokine expression

Wu, Shuling; Korte, Alexander; Kebelmann-Betzing, Christian; Geßner, Reinhard; Henze, Guenter; Seeger, Karl

doi:10.1016/j.leukres.2004.04.018

Cited by 21 publications

(29 citation statements)

References 36 publications

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“…18 Recently, the abundant expression of specific IL-15Ra has been demonstrated by ALL cells. 4,15 Increasing evidence indicates that membranebound IL-15 presented in transpresentation to IL-15Ra-expressing neighboring cells represents a dominant form, stimulating distinct proliferation or survival signals via cellcell contact. 5,19 Furthermore, intracellular and membranebound IL-15 protein also exert intracrine/reverse effects on the cells that generate them, taking part in gene regulation within cells.…”

Section: Discussionmentioning

confidence: 99%

“…15,16 The PCR products were verified by gel electrophoresis and by sequencing (ABI 377, Applied BioSystems, Foster City, Calif).…”

Section: Patientsmentioning

confidence: 99%

“…4,15 Briefly, the purified PCR amplicon concentrations were measured by optical density spectrophotometer and calculated to the corresponding copy number. To minimize interassay variability, all samples analyzed from a single experiment were performed with same dilution series of a standard.…”

Section: Patientsmentioning

confidence: 99%

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Expression of interleukin 15 in primary adult acute lymphoblastic leukemia

Fischer²,

Gökbuget

et al. 2009

Cancer

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BACKGROUND:Interleukin‐15 (IL‐15) has been associated with the growth, survival and biological behavior of leukemic cells and response to therapy. We determined the expression of IL‐15 in lymphoblasts and evaluated its potential impact on the outcome in adult acute lymphoblastic leukemia (ALL).METHODS:Between June 1999 and June 2006, ALL samples were collected from 87 adult patients before initiation of antineoplastic therapy. These patients were enrolled in the German Multicenter Acute Lymphoblastic Leukemia June 1999 and July 2003 study trials. The expression of IL‐15 in leukemic cells was analyzed by real‐time polymerase chain reaction.RESULTS:The expression of IL‐15 correlated with the immunophenotype: T‐lineage ALL had a more than 4‐fold higher IL‐15 mRNA expression as compared with B‐cell precursor (BCP)‐ALL (P < .001). Patients with BCR‐ABL+‐BCP‐ALL had lower IL‐15 expression compared with BCR‐ABL−‐BCP‐ALL (P = .041). Furthermore, higher expression of IL‐15 was associated with mediastinal (P = .001) and lymph node infiltration (P = .051), but not with hepatomegaly and splenomegaly. Notably, high IL‐15 expression in BCP‐ALL was associated with an inferior relapse‐free survival (RFS) at 5 years (0.17 ± 0.13 vs 0.47 ± 0.13) (P = .008), but there was no impact on overall survival (P = .249).CONCLUSIONS:Differential expression of IL‐15 in adult ALL at diagnosis was associated with clinical features and outcome, in particular, RFS. It remains to be evaluated whether IL‐15 might be a relevant therapy target, or might be used for risk stratification. Cancer 2010. © 2010 American Cancer Society.

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Section: Discussionmentioning

confidence: 99%

“…15,16 The PCR products were verified by gel electrophoresis and by sequencing (ABI 377, Applied BioSystems, Foster City, Calif).…”

Section: Patientsmentioning

confidence: 99%

See 1 more Smart Citation

Expression of interleukin 15 in primary adult acute lymphoblastic leukemia

Fischer²,

Gökbuget

et al. 2009

Cancer

Self Cite

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“…[15][16][17] Morphological studies investigating the significance of the BM stroma are rare in childhood ALL. We have previously shown a prognostic impact of BM fibrosis measured as reticulin fiber density (RFD) at diagnosis in childhood ALL.…”

Section: Introductionmentioning

confidence: 99%

Bone marrow fibrosis in childhood acute lymphoblastic leukemia correlates to biological factors, treatment response and outcome

et al. 2007

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“…As well, cytokines are highly expressed from stromal cells from leukemic BM [27]. In in vitro studies on ALL cells, IL-7 cytokine was progression factor [28].…”

Section: Cytokinesmentioning

confidence: 99%

Microenvironment and its role in acquire drug resistance of leukemia treatment

Qattan¹

2016

J Stem Cell Res Med

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Bone marrow niche is the microenvironment where the leukemic cells residue and it support cell proliferation, metastasis, and drug resistance by secretion of numerous factors including cytokines. When leukemic cells grow, they disrupt normal hematopoietic progenitor cell (HPC) bone marrow niches and create abnormal microenvironments. Also, niches recently have been linked to the development of drug resistance. One type of stem cell that resides in the bone marrow niche is called Mesenchymal stem cells (MSCs). Two microenvironment niches have been identified in BM; Osteoblastic and vascular, these niches provide the area in where the leukemia cells can escape from the chemotherapy-induced death and acquire drug resistance cells. Moreover, this review highlight briefly the role of exosomes in cell-to-cell communication and progression. The interaction between BM and leukemia cells through different proteins including cytokines, adhesion molecule, signal transduction pathways and hypoxia are mentioned simply.MSCs have potential successful uses in regenerative medicine, but in this short review focusing on their potential role in supporting resistance in leukemia and the progress in elucidating the cellular components of HSC in BM.

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Interaction of bone marrow stromal cells with lymphoblasts and effects of predinsolone on cytokine expression

Cited by 21 publications

References 36 publications

Expression of interleukin 15 in primary adult acute lymphoblastic leukemia

Expression of interleukin 15 in primary adult acute lymphoblastic leukemia

Bone marrow fibrosis in childhood acute lymphoblastic leukemia correlates to biological factors, treatment response and outcome

Microenvironment and its role in acquire drug resistance of leukemia treatment

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