Interaction of Bishydroxycoumarin with Human Serum Albumin

Cho, M.J.; Mitchell, A.G.; Pernarowski, M.

doi:10.1002/jps.2600600207

Cited by 40 publications

(12 citation statements)

References 25 publications

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“…The driving force for the binding of 4-methoxyphenoxyacetic acid to BSA is the negative standard enthalpy change, a finding in common with those from other studies of the binding of aromatic anions to serum albumin (1,4,9) and consistent with the proposed binding mechanism. Under these circumstances, the large negative standard entropy change could result from the loss of transla tional and rotational motion of the acid molecules which would accompany their binding and from the ordering of the water molecules in the hypothetical hole which forms when the acid molecule vacates the aqueous environment.…”

Section: Discussionsupporting

confidence: 75%

Binding of Some Phenoxyalkanoic Acids to Bovine Serum Albumin <i>in vitro</i>

Mason¹

1975

Pharmacology

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The phenoxyalkanoic acids are bound to bovine serum albumin at a single site of high affinity, where the interaction is most importantly influenced by strong hydrophobic bonding, with ionic bonding being of lesser importance, and also at several sites of lower affinity. The hydrophobic binding site has almost equal affinity for the phenoxy groups of 2,4-dichlorophenoxyacetic acid, 4-(2,4-dichlorophenoxy)butyric acid and 2-(2,4,5-trichlorophenoxy)propionic acid and greater affinity for the phenoxy group of 2,4,5-trichlorophenoxyacetic acid. The properties of the high affinity binding site bear some resemblance to the properties of the amino acid sequence adjoining the tryptophan residue of human serum albumin, and evidence is presented for the presence of tryptophan at the high affinity binding site of bovine serum albumin.

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Section: Discussionsupporting

confidence: 75%

Binding of Some Phenoxyalkanoic Acids to Bovine Serum Albumin <i>in vitro</i>

Mason¹

1975

Pharmacology

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“…RESULTS AND DISCUSSION Spectrophotometric Analysis-Polyvinylpyrrolidone alters the spectral characteristics of bishydroxycoumarin. A similar phenomenon was observed for the human serum albumin-bishydroxycoumarin interaction (2). It was suggested in that paper (2) that the u$-unsaturated lactone structure present in bishydroxycoumarin was involved in the interaction.…”

Section: Methodssupporting

confidence: 55%

“…Materials-Specifications for bishydroxycoumarin, tris(hydroxymethyl)aminoethane, and dialyzer tubing were given in a previous paper (2). Poly~inylpyrrolidone~ was extracted with anhydrous ether in a continuous-extraction apparatus for 24 hr.…”

Section: Methodsmentioning

confidence: 99%

Interaction of Bishydroxycoumarin with Polyvinylpyrrolidone

Cho

Mitchell

Pernarowski

1971

Journal of Pharmaceutical Sciences

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“…Hauser, Phillips & others, 1949;McBain, Huff & Brady, 1949;Thakkar & Wilham, 1970), whereas others (e.g. Lund & Pedersen-Bjergaard, 1949;Goyan, 1949;Hocking, 1951;Few & Schulman, 1953), have not observed surface activity. Surface tension measurements were, therefore, made on solutions of penicillin G and ampicillin in triple-distilled water; 0.05, 0.10, 0.25 and 0.50% w/v solutions of the antibiotics were examined for surface activity using a Du Nouy Tensiometer (Cambridge Instruments Limited), but in no case was significant surface activity detected.…”

Section: Surface Tensionmentioning

confidence: 93%

The diffusion of penicillin G and ampicillin through phospholipid sols

Padfield

Kellaway

1975

Journal of Pharmacy and Pharmacology

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The self-diffusion coefficients of penicillin G and ampicillin have been determined at 25" in water and in the presence of a swamping concentration of electrolyte. The antibiotics diffused through phospholipid dispersions at a reduced rate due to interaction with the lipid aggregates. Ampicillin diffused through the phosphatidylcholine and phosphatidylethanolamine dispersions more rapidly than penicillin G, whereas the latter diffused more rapidly through the lysophosphatidylcholine dispersions. Estimates of binding have been made from these data and compared with those obtained from equilibrium dialysis studies. Surface tension measurements indicated that the antibiotics exhibited minimal surface activity. These results have been correlated with data obtained in other studies and a possible explanation has been advanced for the reported differences in in vivo activity of penicillin G and ampicillin.

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Interaction of Bishydroxycoumarin with Human Serum Albumin

Cited by 40 publications

References 25 publications

Binding of Some Phenoxyalkanoic Acids to Bovine Serum Albumin <i>in vitro</i>

Binding of Some Phenoxyalkanoic Acids to Bovine Serum Albumin <i>in vitro</i>

Interaction of Bishydroxycoumarin with Polyvinylpyrrolidone

The diffusion of penicillin G and ampicillin through phospholipid sols

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