Interaction of Biomaterials Containing Calcium Hydroxyapatite/Poly-L-Lactide With the Simulated Body Fluid

Abstract

The purpose of biomaterials is to replace a part or a function of the body in a safe, physiologically and economically acceptable way. The process of the reconstruction of bone defects has always been a big problem in orthopedics and maxillofacial surgery. Since hydroxyapatite (HAp) was detected as a component, the predominant constituent and the integral element of Mammalian bones, the development of the phospate ceramics as potential materials for implantation was enabled. This study investigated whether and in which way biomaterial calcium hydroxyapatite/poly-L-lactide (HAp/PLLA) interacts with the ionic composition of the human plasma. The simulated body fluid (SBF) is an artificial fluid that has the ionic composition and ionic concentration similar to the human blood plasma. HAp/PLLA was incubated for 1, 2, 3 and 5 weeks in SBF. The surfaces of both treated and untreated materials were analyzed on a scanning electron microscopy (SEM), and were also exposed to the energy dispersive X-ray spectroscopy (EDS), while SBF was submitted to the measuring of pH and electrical conductivity. However, our results indicate that the degradational changes of the material HAp/PLLA in SBF start from the surface of the treated material and that observed changes are the consequence of dissolution of its polymer component and the precipitation of the material similar to hydroxyapatite on its surface. This material shows good characteristics that place it among good candidates for the application in orthopedics and maxillofacial surgery.

Porous hollow hydroxyapatite microspheres synthesized by spray pyrolysis using a microalga template: preparation, drug delivery, and bioactivity

“…This initial increase and subsequent decrease in conductivity following immersion in SBF has also been shown in prior research specic to the immersion of calcium phosphate/poly-DL-lactide-co-glycolide composites in SBF. 48 The changes in pH and conductivity values for the HAp and HHM samples did not show signicant differences. Therefore, in this study, the differences in morphology and the use of a microalga template did not affect the characteristics of apatite formation.…”

Bacterial Cellulose-Chitosan-Glycerol Biocomposite as Artificial Dura Mater Candidates for Head Trauma

“…Degradation test was performed to find out how long the samples could survive in the body when applied. Test method used was in vivo or in vitro performed using Simulated Body Fluid (SBF) solution media [11]. The membrane was immersed in a sterile SBF solution at 37 ° C for 3 to 12 weeks.…”

Molecular designing of nanoparticles and functional materials

“…35 Newly formed bone tissue was transformed into the mature one using the specific forms, islet-like ossification centres. Original and innovative systems based on PLGA nano-spheres--coated HAp individually 36 or as carriers of various antibiotics and vitamins, justified their further development and the expansion of potential application. The radioactive 125 iodine ( 125 I), a low energy gamma emitter, was used to develop a novel in situ method for the radiolabelling of polymer-coated and uncoated HAp nanoparticles and the investigation of their biodistribution.…”