Interaction of beta-lactoglobulin and bovine serum albumin with iron oxide (α-Fe₂O₃) nanoparticles in the presence and absence of pre-adsorbed phosphate

Abstract

Protein adsorption onto mineral nanoparticle surfaces is critical to the function and fate of biological compounds in environmental and industrial systems. However, adsorption kinetics, coverage, and conformation of biological macromolecules...

Nanoparticle protein corona: from structure and function to therapeutic targeting

“…Likewise, phosphate pre-adsorption on hematite (α-Fe 2 O 3 )NPs reduces protein surface coverage, slows the protein-specific kinetics of BSA and β-LG, and restricts secondary structural changes in proteins. 94 Phosphate's ability to attenuate the α-Fe 2 O 3 NP-induced secondary structural changes of proteins is attributed to its induction of steric constraints or bridging and ternary complex formation, suggesting phosphate as a potential agent in attenuating adsorbed protein denaturation, particularly at low surface coverage.…”

“…At pH 6, HM NPs induce protein-specific secondary structural changes in adsorbed BSA and beta-lactoglobulin (β-LG), followed by proteins returning to their solution-phase conformation after 90 minutes. 94 However, phosphate pre-adsorption on HM NPs reduces protein surface coverage, slows the protein-specific kinetics of BSA and β-LG, and restricts secondary structural changes in proteins. The ability of phosphate to attenuate the HM NP-induced secondary structural changes of proteins can be attributed to its induction of steric constraints or bridging and ternary complex formation, suggesting phosphate as a potential agent in attenuating adsorbed protein denaturation, particularly at low surface coverage.…”

Analysis of micro- and nanoscale heterogeneities within environmentally relevant thin films containing biological components, oxyanions and minerals using AFM-PTIR spectroscopy

“…Hydrogen bonding and other nonionic interactions most likely comprise these interactions. [43][44][45] Comparing the BSA and goethite spectrum (light green) in Fig. 3 to that of BSA and goethite alone (Fig.…”

The effects of different iron and phosphorus treatments on the formation and morphology of iron plaque in rice roots (Oryza sativa L)

“…When Fe 2+ is present in high concentrations, ferrihydrite undergoes a dissolution-recrystallization process to form goethite ( Sheng et al., 2020 ). Phosphate coats the surface of goethite to impede both structural transformations and morphological evolution processes ( Ustunol et al., 2021 ). Both Fe 2+ and PO 4 3- play crucial roles in the growth of rice and the formation of iron plaques ( Giannopolitis and Ries, 1977 ; Amako et al., 1994 ; Sheng et al., 2020 ; Ustunol et al., 2021 ).…”