Interaction of Bacterial and Lambda Phage Recombination Systems in the X-Ray Sensitivity of
            <i>Escherichia coli</i>
            K-12

Trgovčević, Željko; Wd, Rupp

doi:10.1073/pnas.71.2.503

Cited by 16 publications

(16 citation statements)

References 23 publications

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“…However, the simultaneous presence of Gam and the RecBCD enzyme appreciably increases X-ray resistance of the bacterial cells (41,42). This increased resistance is associated with the enhanced cellular capacity for the repair of radiationinduced strand breaks (42).…”

Section: Resultsmentioning

confidence: 99%

Modulation of EcoKI restriction in vivo: role of the lambda Gam protein and plasmid metabolism

et al. 1997

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Two novel types of alleviation of DNA restriction by the EcoKI restriction endonuclease are described. The first type depends on the presence of the gam gene product (Gam protein) of bacteriophage . The efficiency of plating of unmodified phage is greatly increased when the restricting Escherichia coli K-12 host carries a gam ؉ plasmid. The effect is particularly striking in wild-type strains and, to a lesser extent, in the presence of sbcC and recA mutations. In all cases, Gam-dependent alleviation of restriction requires active recBCD genes of the host and recombination (red) genes of the infecting phage. The enhanced capacity of Gam-expressing cells to repair DNA strand breaks might account for this phenomenon. The second type is caused by the presence of a plasmid in a restricting host lacking RecBCD enzyme. Commonly used plasmids such as the cloning vector pACYC184 can produce such an effect in strains carrying recB single mutations or in recBC sbcBC strains. Plasmid-mediated restriction alleviation in recBC sbcBC strains is independent of the host RecF, RecJ, and RecA proteins and phage recombination functions. The presence of plasmids can also relieve restriction in recD strains. This effect depends, however, on the RecA function in the host. The molecular mechanism of the plasmid-mediated restriction alleviation remains unclear.

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Section: Resultsmentioning

confidence: 99%

Modulation of EcoKI restriction in vivo: role of the lambda Gam protein and plasmid metabolism

et al. 1997

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“…22,24 They noted that expression of lysogen-induced gam in wildtype cells made the cells more resistant to X-rays than uninduced cells. Initial explanations for this observation included: (1) a differential inhibition of RecBCD by Gam activities; (2) a partial inhibition of RecBCD by Gam resulting in lower rates of DNA degradation; and (3) a Gam-modified enzyme with a new activity.…”

mentioning

confidence: 98%

The λ Gam Protein Inhibits RecBCD Binding to dsDNA Ends

Murphy

2007

Journal of Molecular Biology

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“…The drug resistance factor R46 was obtained in a host strain which was lysogenic for bacteriophage A (strain 24 from Dr E. Meynell). As the products of the red genes of h can effect the survival of the host after irradiation (Trgovcevic & Rupp, 1974) and can increase the yield of recombinants (Signer & Weil, 1968;Lloyd, 1971 ;Oliver, unpublished results), the plasmid was first transferred to the non-lysogenic A-resistant strain, ZF-. From this strain the plasmid was transferred into the strains used in this investigation.…”

Section: Bacteria and Bacteriophagementioning

confidence: 99%

The Effect of a Drug-resistance Factor on Recombination and Repair of DNA in Escherichia coli K12

Oliver

Stacey

1977

Journal of General Microbiology

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The presence in recipient strains of Escherichia coli K 1 2 of the plasmid R46 greatly reduced the yield of recombinants from crosses with several Hfr strains and virtually abolished the formation of recombinants by P I transduction without, however, significantly affecting the transfer of the F prime from a strain carrying Fgal. The R46 plasmid had paradoxical effects on mutability: it appeared to enhance the yield of mutants following irradiation with ultraviolet light but it reduced the number of mutants detectable in unirradiated cultures. The effects of this plasmid on ultraviolet light survival of the wild type and several mutants defective for recombination and repair have been measured and the results, in the main, confirm similar observations by Tweats et al. (1976). Not only is the survival of the strain harbouring R46 greater than that of the parent strain in all the cases studied, but the survival of ultraviolet irradiated bacteriophage h is also greater. I N T R O D U C T l O NA number of enzymic mechanisms have been suggested for the processes of repair and recombination of DNA in the bacterium Escherichia coli K 1 2 (for reviews see Grossman eta/., 1975;Clark, 1973). In addition to the chromosomal gene functions involved, identified by rec, uvr, lex and sbc mutations, a number of extrachromosomal elements appear to be able to influence the repair and recombination of DNA. The increased survival after ultraviolet (u.v.) irradiation of strains carrying ColI compared with their non-colicinogenic parent strains was first described by Howarth (1965) and the effects of other bacterial plasmids which confer on their hosts an increased resistance to DNA damaging agents have been discussed (Drabble & Stocker, 1968;Marsh & Smith, 1969;Siccardi, 1969). With the increased survival there is a concomitant increase in the induced mutation rate (Krishnapillai, I 975) and in Salmonella typhimurium the spontaneous mutation rate is also increased (Mortelmans & Stocker, personal communication). However, the mechanism(s) responsible for these phenomena are unknown although one plasmid, R-Utrecht, has been shown to code for a new DNA polymerase (MacPhee, 1974). As certain of the gene products that are involved in the repair of DNA are also required for recombination, the effect of R46 (= R1818 = R-Brighton), a plasmid which increases the resistance of S. typhimurium to U.V. light, was investigated. In this paper the effects of this plasmid on U.V. survival and recombination in wild-type strains and in certain mutant strains are reported. With the exception of the results relating to recA strains, the data confirm and extend the observations concerned with the U.V. survival of R46+ strains reported by Tweats et al.

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Interaction of Bacterial and Lambda Phage Recombination Systems in the X-Ray Sensitivity of Escherichia coli K-12

Cited by 16 publications

References 23 publications

Modulation of EcoKI restriction in vivo: role of the lambda Gam protein and plasmid metabolism

Modulation of EcoKI restriction in vivo: role of the lambda Gam protein and plasmid metabolism

The λ Gam Protein Inhibits RecBCD Binding to dsDNA Ends

The Effect of a Drug-resistance Factor on Recombination and Repair of DNA in Escherichia coli K12

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