Interaction of Anticancer Drug Methyl Caffeate with DNA Investigated by Electrochemical and Spectroscopic Methods

Abstract

The interaction of methyl caffeate with herring sperm DNA was investigated by cyclic voltammetry, differential pulse voltammetry and ultraviolet absorption spectrometry. Methyl caffeate had a well-defined electrochemical signal in 0.50 M acetate buffer (pH 4.6) involving two electrons and two protons. The binding of methyl caffeate to herring sperm DNA resulted in a series of changes in the electrochemical behavior, giving a considerable decrease in the peak currents and a negative shifts in the peak potential of methyl caffeate, suggesting the interaction of methyl caffeate and herring sperm DNA by an electrostatic mode. Furthermore, the data from the electrochemical investigation showed that a non-electroactive supramolecular compound was formed between methyl caffeate and herring sperm DNA. The conclusion was reinforced by ultraviolet absorption spectrometry.

Multiscale Modeling Unravels the Influence of Biomembranes on the Photochemical Properties of Embedded Anti-Oxidative Polyphenolic and Phenanthroline Chelating Dyes

“…As shown in Figure a, the high-frequency OPA Caffest spectrum in vacuum contains at least one of the peaks of the methyl caffeate by Wang et al at 213 and 300 nm. For example, the band at 300 nm present in our panoramic OPA absorption spectrum in Figure is also identically present at the high-frequency outlier in Figure a, which in addition has a shoulder at ca.…”

“…This is an indication that the low-frequency outlier conformation also has a secondary high-frequency transition component. The conformation As shown in Figure 8a, the high-frequency OPA Caffest spectrum in vacuum contains at least one of the peaks of the methyl caffeate by Wang et al 67 at 213 and 300 nm. For example, the band at 300 nm present in our panoramic OPA absorption spectrum in Figure 5 is also identically present at the high- 3).…”