Interaction of anthracycline antibiotics with biopolymers: 7. Equilibrium binding studies on the interaction of iremycin and DNA

Walter, Axel; Schultz, Harry P.; Stutter, E.

doi:10.1016/0141-8130(83)90059-4

Cited by 13 publications

(7 citation statements)

References 14 publications

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“…1) and intercalates between DNA base pairs in solution (25,26). The binding site of anthracyclines covers 3-4 DNA base pairs (27). The high value of ni = 18 + 8 indicates a strong suppression of the conformational flexibility of DNA by the drug.…”

Section: Resultsmentioning

confidence: 99%

Infrared linear dichroism of oriented DNA-ligand complexes prepared with the wet-spinning method

Fritzsche¹,

Rupprecht

Richter³

1984

Nucl Acids Res

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Oriented DNA films prepared by the wet-spinning technique have been complexed with several ligands: the anthracycline antibiotic violamycin BI, the dipeptide L-carnosine, and the oligopeptide antibiotic netropsin. The formation of the DNA-ligand complexes is accompanied by dramatic changes of the conformational flexibility of DNA. The B-A transition which occurs usually between 80% and 70% relative humidity (RH) is more or less suppressed by the ligands. Violamycin BI at a total ligand per DNA base pair ratio, r , of '0.03 and L-carnosine at rt X' 1.5 inhibit the B-A transition of "'18 and "'0.25 base pairs per ligand molecule, respectively. Netropsin at rt = 0.2 induces a very stable B-DNA even at rather low RH (23 %). The total hydration of this complex is significantly higher than for a drug-free DNA film. Netropsin-DNA complexes at r of 0.02 and 0.01 result in an inhibition of "45 base pairs pe4 drug molecule with respect to the B-A transition.

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Section: Resultsmentioning

confidence: 99%

Infrared linear dichroism of oriented DNA-ligand complexes prepared with the wet-spinning method

Fritzsche¹,

Rupprecht

Richter³

1984

Nucl Acids Res

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“…From a set of several such titrations, differing in DNA concentration, the equilibrium binding parameters were calculated using the above-mentioned binding model. The corresponding numerical fitting procedure of the orginal titration data was performed for ,B-rhodomycin-I (and recentl y for iremycin (WALTER et al 1983)) with the absorbances a t wavenumber v2 (absorption maximum), whereas for 8-rhodomycin-I1 the measurements at v3 were additionally included. Moreover, to test the internal consistency of the parameter estimations, for @-rhodoniycin-I1 an additional set of data, obtained by titration experiments with independently prepared stock solutions of ligand and of calf-thymus DNA, was used.…”

Section: Equilibrium Bi?zdi?zgmentioning

confidence: 99%

“…in the weighing-in concentrations of ligand and/or polymer) are not caught. Table 2 summarizes the equilibrium Table 2 Equilibrium binding parameters of iremycin (WALTER et al 1983, SCIXUTZ et al 1983 ( 1 5 7 ) ( 5 3 6 ( 1 8 7 ) ( 1 5 3 ) DNA binding parameters obtained for the two /I-rhodomycins regarding the dimerization of the ligands. The error limits without parentheses in Table 2 were estimated neglecting the errors of the dimerization parameters from Table 1, whereas those in paraiitheses were obtained with consideration of error propagation.…”

Section: Equilibrium Bi?zdi?zgmentioning

confidence: 99%

“…Comparative studies of the antimicrobial activity of iremycin and adriamycin to different bacterial strains and to the inhibition of &phage multiplication (FLECK etal. 1982) led to ratios of activity which were in the order of the ratios of the corresponding DNA binding constants of these drugs (WALTER et al 1983). More recently, from the iremycin-producing strains (SCHLEGEL et al.…”

mentioning

confidence: 98%

“…1979), carminomycin (F~RSTER et al 198413) and iremycin (WALTER et al 1983) and the kinetics of complex formation of these drugs (FKRSTER et al 1984a, b) were investigated. A proportionality (FORSTER et al 198410) was found between the constant K of the singly contiguous binding of the ligand (Mc &TEE et al 1974) and the mean attachment time of the drug which is the reciprocal of the corresponding dissociation rate constant kD.…”

mentioning

confidence: 99%

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Interaction of anthracycline antibiotics with biopolymers: Comparative studies of DNA binding and antimicrobial activity of rhodomycin‐type anthracycline antibiotics

Stutter¹,

Walter²,

Fleck³

1986

J. Basic Microbiol.

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The binding of the anthracyclines beta-rhodomycin-I and beta-rhodomycin-II to calf thymus DNA was investigated by both equilibrium and kinetic methods taking into account ligand dimerization (ionic strength I = 0.2 M, pH 6.0). The analysis was based upon a cooperative single-step binding mechanism with overlapping of potential binding sites on a linear homogeneous lattice. Equilibrium binding parameters were estimated from spectrophotometric titration experiments by means of a nonlinear fitting program. The results were compared with those obtained previously for the related antibiotic iremycin and were complemented by kinetic parameters determined from temperature-jump experiments at high binding ratio. The binding constants and the mean attachment times of the drugs were found to increase in the serial order iremycin, beta-rhodomycin-I and beta-rhodomycin-II, which is in line with their increasing antimicrobial activity on Bacillus subtilis ATCC 6633.

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Characterization of an oligonucleotide‐binding fluorescent ligand for application in affinity purification of dsDNA

Haynes

Sherwood

Turner

1995

Biotech & Bioengineering

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The fluorescent probe PO-PRO-3 was investigated as a potential ligand for the affinity immobilization and purification of genomic or plasmid DNA fragments. Affinities and mechanisms for PO-PRO-3 binding to superhelical and linearized pUC 18 plasmid DNA were examined through measurement of binding isotherms, continuous-variation analysis, and DNA titrations. In addition, the effects of DNA conformation, protein and RNA contaminants, solvent polarity, and ionic strength are examined with the aim of optimizing binding and elution conditions and of assigning limits to the range of applicability of the affinity purification.

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Interaction of anthracycline antibiotics with biopolymers: 7. Equilibrium binding studies on the interaction of iremycin and DNA

Cited by 13 publications

References 14 publications

Infrared linear dichroism of oriented DNA-ligand complexes prepared with the wet-spinning method

Infrared linear dichroism of oriented DNA-ligand complexes prepared with the wet-spinning method

Interaction of anthracycline antibiotics with biopolymers: Comparative studies of DNA binding and antimicrobial activity of rhodomycin‐type anthracycline antibiotics

Characterization of an oligonucleotide‐binding fluorescent ligand for application in affinity purification of dsDNA

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