Interaction of alveolar macrophages from pulmonary patients and healthy subjects with liposomes

Abstract

Formation of primary metabolites of oxidative burst in alveolar macrophages from healthy subjects and patients with chronic lung diseases is studied using the method of luminoldependent chemiluminescence. Incubation with liposomes composed from phosphatidylcholine and a phosphatidylcholine--fatty acid esters mixture induces similar activation of oxidative burst in alveolar macrophages from healthy subjects and patients with lung diseases. In the present study we evaluated the formation of primary oxidative burst metabolites in alveolar macrophages (AM) from healthy subjects and patients with chronic lung diseases incubated with liposomes composed from phosphatidylcholine (PC) alone or in combination with fatty acid esters (FAE) enriched with arachidonic acid. MATERIALS AND METHODSBronchoscopy was carried out under local anesthesia (lidocaine) using a standard method.Bronchial lavage was fdtered through cotton wool and centrifuged for 10 min at 400g and 4~ The pellet was suspended in 4 ml Hanks' solution, layered on Isopaque,Ficoll (1.076 g/Inl), and centrifuged for 20 min at 1500 rpm and 22~ The top macrophage-containing layer (0.5-0.7 ml) was centrifuged at 150 rpm and room temperature for 5-10 min. The pellet was suspended in 2 1111 Hanks' solution, layered on a PercoU gradient (1.123, 1.077, and 1.043 g/ml), and centrifuged at 400g for 10 mill. The top layer was collected, centrifuged, and suspended