Interaction of 2C T Cells with a Hybrid L^d Molecule Bearing an α3 Domain Derived from the Class Ib Molecule, Qa‐2

Abstract

Thus, either CD8 is not required for negative selection of this TCR or a weak interaction of CD8 with L Q3 is sufficient. TSA-1, a developmentally regulated marker, was used to follow the process of negative selection. The results show that deletion of 2C T cells does not occur until thymocytes reach the double positive (DP) stage. Furthermore, the authors noted a small population of DP TSA-1 hi cells remains, while DP TSA-1 int and TSA-1 lo cells are absent. These data support the notion that thymocytes either reach a particular stage of development or locate in an appropriate intrathymic compartment before they undergo negative selection.