Interaction effects of Fusarium enniatins (A, A1, B and B1) combinations on in vitro cytotoxicity of Caco-2 cells

Prosperini, Alessandra; Font, Guillermina; Ruiz, María-José

doi:10.1016/j.tiv.2013.06.021

Cited by 57 publications

(40 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These combinations led to highly significant reductions of TEER after 24, 48 and 72 h, pointing towards an additive effect. This is in accordance with Prosperini et al [106], who tested a quaternary mixture of enniatin A, A1, B and B1 on viability of Caco-2 cells, with each toxin applied at 0.3125, 0.75, 1.25, or 2.5 µM. Authors concluded a general additive effect.…”

Section: Discussionsupporting

confidence: 90%

“…The choice of realistic in vitro concentrations is therefore difficult. Concentrations of enniatins used for this study were based on an approach by Prosperini et al [106], who concluded that for other fusariotoxins such as fumonisins, the maximum levels in raw cereals reach approximately 2000 µg/kg. Taking this value into consideration and considering cereal consumption in the European Union to be 125.4 kg/year, the group estimated that Europeans consumed approximately 680 µg fumonisins per day, and applied this calculation to enniatins, for example enniatin A1.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Fusarium-Derived Metabolites on the Barrier Integrity of Differentiated Intestinal Porcine Epithelial Cells (IPEC-J2)

Springler

Vrubel²,

Mayer³

et al. 2016

Toxins

View full text Add to dashboard Cite

The human, animal and plant pathogen Fusarium, which contaminates agricultural commodities worldwide, produces numerous secondary metabolites. An example is the thoroughly-investigated deoxynivalenol (DON), which severely impairs gastrointestinal barrier integrity. However, to date, the toxicological profile of other Fusarium-derived metabolites, such as enniatins, beauvericin, moniliformin, apicidin, aurofusarin, rubrofusarin, equisetin and bikaverin, are poorly characterized. Thus we examined their effects—as metabolites alone and as metabolites in combination with DON—on the intestinal barrier function of differentiated intestinal porcine epithelial cells (IPEC-J2) over 72 h. Transepithelial electrical resistance (TEER) was measured at 24-h intervals, followed by evaluation of cell viability using neutral red (NR) assay. Enniatins A, A1, B and B1, apicidin, aurofusarin and beauvericin significantly reduced TEER. Moniliformin, equisetin, bikaverin and rubrofusarin had no effect on TEER. In the case of apicidin, aurofusarin and beauvericin, TEER reductions were further substantiated by the addition of otherwise no-effect DON concentrations. In all cases, viability was unaffected, confirming that TEER reductions were not due to compromised viability. Considering the prevalence of mycotoxin contamination and the diseases associated with intestinal barrier disruption, consumption of contaminated food or feed may have substantial health implications.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Effect of Fusarium-Derived Metabolites on the Barrier Integrity of Differentiated Intestinal Porcine Epithelial Cells (IPEC-J2)

Springler

Vrubel²,

Mayer³

et al. 2016

Toxins

View full text Add to dashboard Cite

show abstract

“…ENs and BEA possess a wide range of potential properties: antimicrobial, anthelmintic, insecticidal, antifungal, herbicidal, phytotoxic, immunosuppressive, and cytotoxic [13,14]. Due to their similar chemical structures, ENs and BEA can present additive or synergistic cytotoxic effects, as has been demonstrated by in vitro studies [15,16]. …”

Section: Introductionmentioning

confidence: 99%

Presence of Enniatins and Beauvericin in Romanian Wheat Samples: From Raw Material to Products for Direct Human Consumption

Stanciu

Juan

Miere

et al. 2017

Toxins

View full text Add to dashboard Cite

In this study, a total of 244 wheat and wheat-based products collected from Romania were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS) in order to evaluate the presence of four enniatins (ENs; i.e., ENA, ENA1, ENB, and ENB1) and beauvericin (BEA). For the wheat samples, the influence of agricultural practices was assessed, whereas the results for the wheat-based products were used to calculate the estimated daily intake of emerging mycotoxins through wheat consumption for the Romanian population. ENB presented the highest incidence (41% in wheat and 32% in wheat-based products), with its maximum levels of 815 μg kg−1 and 170 μg kg−1 in wheat and wheat-based products, respectively. The correlation between the concentrations of ENB and ENB1 in wheat grain samples and farm practices (organic or conventional) was confirmed statistically (p < 0.05). This is the first study that provides comprehensive information about the influence of agricultural practice on emerging Fusarium mycotoxin presence in Romanian wheat samples and the estimated daily intake of ENs and BEA present in wheat-based products for human consumption commercialized in Romania.

show abstract

“…Enniatins (ENs) and beauvericin (BEA) are a group of Fusarium mycotoxins receiving importance in the last years. Although adverse effects have not been attributed to these mycotoxins in humans, different toxicological studies have evidenced the cytotoxic activity of ENs and BEA (alone or in combination) lower than micromolar range (Prosperini et al 2014). The human exposure to ENs and BEA commonly occurs through different cereals and their products, such as bread, pasta, or biscuits (Serrano et al 2013a, b;Capriotti et al 2014;Hu et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Dispersive Liquid-Liquid Microextraction for the Determination of Emerging Fusarium Mycotoxins in Water

et al. 2015

Self Cite

View full text Add to dashboard Cite

A new method was optimized for the determination of emerging Fusarium mycotoxins enniatins (ENs) and beauvericin (BEA) in different types of water. Mycotoxin analysis was performed by dispersive liquid-liquid microextraction (DLLME) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Mycotoxins were efficiently extracted from water into carbon tetrachloride by DLLME technique using acetonitrile as disperser solvent. Detection limits were in the range of 0.06-0.17 μg L −1 . Quantification was performed using matrixmatched calibration curves over a linear range from limit of quantification (LOQ) to 200 μg L −1 . Acceptable recoveries were obtained between 78.5 and 100.1 % with relative standard deviations of <14 %. The proposed method may be advised as an easy, sensitive, and accurate method for determining emerging Fusarium mycotoxins in water. The method was successfully applied to the analysis of different kinds of water. No detectable levels were achieved in surface, ground, tap, and bottled water. Concentration levels up to 50 μg L −1 were detected in cooking water related to the pasta cooking process.

show abstract

Interaction effects of Fusarium enniatins (A, A1, B and B1) combinations on in vitro cytotoxicity of Caco-2 cells

Cited by 57 publications

References 29 publications

Effect of Fusarium-Derived Metabolites on the Barrier Integrity of Differentiated Intestinal Porcine Epithelial Cells (IPEC-J2)

Effect of Fusarium-Derived Metabolites on the Barrier Integrity of Differentiated Intestinal Porcine Epithelial Cells (IPEC-J2)

Presence of Enniatins and Beauvericin in Romanian Wheat Samples: From Raw Material to Products for Direct Human Consumption

Dispersive Liquid-Liquid Microextraction for the Determination of Emerging Fusarium Mycotoxins in Water

Contact Info

Product

Resources

About