Interaction between wild-type, mutant and revertant forms of the bacterium Streptococcus sanguis and the bacterium Actinobacillus actinomycetemcomitans in vitro and in the gnotobiotic rat

Hillman, Jeffrey D.; Shivers, Myra

doi:10.1016/0003-9969(88)90196-3

Cited by 43 publications

(44 citation statements)

References 24 publications

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“…170 These workers demonstrated that the previously observed ability of 5. sanguis to inhibit the growth of A. actinomycetemcomitans was associated with the ability of 5. sanguis to produce hydrogen peroxide at levels that were inhibitory to A. actinomycetemcomitans. A actinomycetemcomitans.…”

Section: G Actinobacillusmentioning

confidence: 68%

Genetic Approaches to The Study of Oral Microflora: A Review

Macrina

Dertzbaugh

Halula

et al. 1990

Critical Reviews in Oral Biology & Medicine

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As the study of oral microorganisms intensified almost 2 decades ago, the application of genetic techniques resulted in important contributions to the understanding of this clinically and ecologically important group of bacteria. The isolation and characterization of mutants of cariogenic streptococci helped to focus attention on traits that were important in colonization and virulence. Such classic genetic approaches gave way to molecular genetic techniques, including recombinant DNA methodology in the late 1970s. Gene cloning systems and methods to move DNA into cells have been developed for oral streptococci. Many streptococcal genes thought to be important in colonization and virulence have since been cloned and their nucleotide sequence determined. Mutant strains have been constructed using defective copies of cloned genes in order to create specific genetic lesions on the bacterial chromosome. By testing such mutants in animal models, a picture of the cellular and molecular basis of dental caries is beginning to emerge. These modern genetic methodologies also are being employed to develop novel and efficacious cell-free or whole cell vaccines against this infection. Genetic approaches and analyses are now being used to dissect microorganisms important in periodontal disease as well. Such systems should be able to exploit advances made in genetically manipulating related anaerobes, such as the intestinal Bacteroides. Gene cloning techniques in oral anaerobes, Actinomyces and Actinobacillus, are already beginning to pay dividends in helping understand gene structure and expression. Additional effort is needed to develop facile systems for genetic manipulation of these important groups of microorganisms.

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Section: G Actinobacillusmentioning

confidence: 68%

Genetic Approaches to The Study of Oral Microflora: A Review

Macrina

Dertzbaugh

Halula

et al. 1990

Critical Reviews in Oral Biology & Medicine

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“…Based on murine studies, it is likely that A. actinomycetemcomitans is exposed to H 2 O 2 produced by streptococci in the oral cavity (14). Why would A. actinomycetemcomitans respond to H 2 O 2 by enhancing resistance to complement killing?…”

Section: Discussionmentioning

confidence: 99%

“…These oral streptococci are typically nonpathogenic and rapidly consume sugars within the subgingival crevice, producing the metabolites lactic acid and hydrogen peroxide (H 2 O 2 ). This physiological ability renders oral streptococci extremely competitive in the oral environment because they consume highenergy carbon sources and excrete metabolites that inhibit growth of neighboring microbes (14).…”

mentioning

confidence: 99%

Polymicrobial interactions stimulate resistance to host innate immunity through metabolite perception

Ramsey

Whiteley

2009

Proc. Natl. Acad. Sci. U.S.A.

153

161

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Bacteria in the human oral cavity often grow in an attached multispecies biofilm community. Members of this community display defined interactions that have an impact on the physiology of the individual and the group. Here, we show that during coculture growth with streptococci, the oral pathogen Aggregatibacter actinomycetemcomitans displays enhanced resistance to killing by host innate immunity. The mechanism of resistance involves sensing of the streptococcal metabolite hydrogen peroxide by A. actinomycetemcomitans, which stimulates a genetic program resulting in enhanced expression of the complement resistance protein ApiA. The oxidative stress response regulator OxyR mediates induction of apiA transcription, and this induction is required for coculture resistance to killing by human serum. These findings provide evidence that interaction between community members mediates prokaryotic resistance to host innate immunity and reinforce the need to understand how polymicrobial growth affects interaction with the host immune system.Aggregatibacter ͉ ApiA ͉ complement ͉ peroxide ͉ Streptococcus gordonii

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“…Most of the bacteria in oral cavity can synthesize bacteriocins (20), quorum sensing molecules (unique communication mechanism of bacteria) (21), and metabolic end-products (22) to gain an advantage in a competitive environment like oral cavity. Some of these metabolites are short chain fatty acids (SCFAs) like acetic acid, butyric acid, propionic acid.…”

Section: Introductionmentioning

confidence: 99%

Growth behavior of eikenella corrodens and streptococcus gordonii in response to a short chain fatty acid metabolite-acetic acid

Yüce¹,

Tülü²,

Inis³

et al. 2017

J Turgut Ozal Med Cent

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Aim: Periodontal diseases are chronic, inflammatory and infectious diseases. Therefore, periodontal treatment aims to eliminate periodontopathogenic bacteria causing periodontal diseases. The aim of present study was to evaluate the effect of a bacterial end metabolite, acetic acid, on periodontopathogenic bacteria, Streptococcus gordonii and Eikenella corrodens. Material and Method: In present research, Eikenella corrodens (ATCC® 23834™) and Streptococcus gordonii (NCTC 7870) were tested. Acetic acid was used in 5% concentration dissolved in distilled water. Negative control agent was distilled water and positive control agents were 0.012% chlorhexidine, penicillin, tetracycline and ciprofloxacin. The antibacterial efficacy of acetic acid against bacteria was tested via disc-diffusion method, MIC test and minimum bactericidal concentration tests. Results: The inhibition zone of ciprofloxacin, penicillin, tetracycline, CHX and acetic acid against Eikenella corrodens and Streptococcus gordonii were 32 and 37 mm, 16 and 14 mm, 21 and 16 mm, 13 and 17 mm, and 14 and 11 mm respectively. Ciprofloxacin and penicillin inhibited bacterial growth in MIC and MBC tests against both bacteria. MIC tests of acetic acid and chlorhexidine against Eikenella corrodens revealed inhibitory effect at 7.81 µl/mL and 0.97 µl/mL concentrations, respectively. Against Streptococcus gordonii, MIC of acetic acid and chlorhexidine were 1.95 µl/mL and 3.90 µl/mL, respectively. Conclusion: Acetic acid is a bacterial end product and has a daily consumption as vinegar. Due to the antibacterial efficacy against periodontopathogenic bacteria, it can be useful in adjunction to periodontal treatment. Further studies to evaluate clinical use of acetic acid as mouthwash, dentifrice, gel and/or irrigation agent are necessary.

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Interaction between wild-type, mutant and revertant forms of the bacterium Streptococcus sanguis and the bacterium Actinobacillus actinomycetemcomitans in vitro and in the gnotobiotic rat

Cited by 43 publications

References 24 publications

Genetic Approaches to The Study of Oral Microflora: A Review

Genetic Approaches to The Study of Oral Microflora: A Review

Polymicrobial interactions stimulate resistance to host innate immunity through metabolite perception

Growth behavior of eikenella corrodens and streptococcus gordonii in response to a short chain fatty acid metabolite-acetic acid

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