Interaction between two cellular subpopulations of a rat colonic carcinoma when inoculated to the syngeneic host

Abstract

From a single, chemically-induced rat colonic carcinoma, two subpopulations of tumor cells have been isolated. When injected into syngeneic rats, TR cells give rise to progressive tumors in most of the animals, whereas TS cells give rise to no tumors or to tumors which regress in less than 30 days. TS cells inhibit the growth of TR tumors, whether they are injected before TR cells or simultaneously, at a different site or mixed with the TR cells. Lymph nodes and spleen lymphocytes from animals having rejected TS tumors also inhibit TR-cell growth. On the other hand, TS cells are able to give rise to progressive tumors when they are injected into rats bearing established TR tumors. Lymph nodes and spleen cells of TR tumor-bearing rats are able to enhance TS cell growth. These results suggest that subpopulations of cancer cells contained in the same tumor interact with each other through their effect on the host immune system. The growth of a whole tumor probably depends not only on the growth potential of each constituent subpopulation, but also on the interaction between the subpopulations themselves.

Differential regulation of HSP27 oligomerization in tumor cells grown in vitro and in vivo

“…These cells yielded immunogenic and regressive tumors when injected in syngeneic animals (Martin et al, 1983;Caignard et al, 1985). The cells were therefore named REG for their regressive phenotype.…”

“…Colorectal cancer REG cells (Martin et al, 1983;Caignard et al, 1985) were grown as monolayers in a controlled atmosphere (378C, 5% CO 2 ) using Ham's F10 medium (BioWhittaker, Fontenay-sous-bois, France) supplemented with 10% (v/v) fetal calf serum. Noncon¯uent cells (exponentially growing cells) were obtained by seeding 2610 6 cells in a 50 ml Falcon¯ask (Falcon, Lincoln Park, NY, USA).…”

Expression of Bcl-XS alters cytokinetics and decreases clonogenic survival in K12 rat colon carcinoma cells

“…Cell culture K12 cells were obtained from Francois Martin and maintained in DMEM medium (Gibco/BRL) supplemented with calf serum to 10% (v/v) and L-glutamine to 2 mM (Caignard et al, 1985). FNX-ampho cells (a derivative of 293T cells) were obtained from Dr Gary P Nolan and were grown in DMEM medium (Gibco/BRL) supplemented with fetal bovine serum to 10% (v/v) and L-glutamine to 2 mM.…”

Analysis of tumour cell composition in tumours composed of paired mixtures of mammary tumour cell lines

“…Other interactions in which the growth of a sister subpopulation is suppressed have been described both in vitro and in vivo (B. E Miller et al, 1980;Caignard et al, 1985;Newcomb et al, 1978) by mechanisms invoking host immtune involvement (B.E. Miller et al, 1980;Caignard et al, 1985;Newcomb et al, 1978) and by mechanisms not involving host immunity .…”

“…E Miller et al, 1980;Caignard et al, 1985;Newcomb et al, 1978) by mechanisms invoking host immtune involvement (B.E. Miller et al, 1980;Caignard et al, 1985;Newcomb et al, 1978) and by mechanisms not involving host immunity . Interactions in which the growth of a sister subpopulation is enhanced, have also been described (Caignard et al, 1985;Brodt et al, 1985;Butler et al, 1983;Tofilon et al, 1984), as well as, in some instances, the tendency for two subpopulations to form a particular final ratio (Leith et al, 1985;Jansson & Revesz, 1976).…”