Interaction between toxic dinoflagellate Alexandrium catenella exposure and disease associated with herpesvirus OsHV-1 μVar in Pacific oyster spat Crassostrea gigas

Lassudrie, Malwenn; Soudant, Philippe; Nicolas, Jean‐Louis; Fabioux, Caroline; Lambert, Christophe; Miner, Philippe; Grand, Jacqueline Le; Petton, Bruno; Hégaret, Hélène

doi:10.1016/j.hal.2015.04.007

Cited by 25 publications

(21 citation statements)

References 61 publications

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“…The SI was then (1) directed towards recipient oysters held at 4 salinities with or without prior acclimation or (2) brought to a salinity of 10 or 25 ‰ and directed towards recipient oysters maintained at 25 ‰. Overall, this method, which has been used in many previous studies (Petton et al 2013, 2015a,b, Tamayo et al 2014, Lassudrie et al 2015, Lemire et al 2015, Pernet et al 2015, reproduces the natural infection process and takes into account the fact that OsHV-1 triggers oyster mortality, although other microorganisms can also play a role (Saulnier et al 2010, Lemire et al 2015, Petton et al 2015b.…”

Section: Introductionmentioning

confidence: 99%

Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1)

Fuhrmann¹,

Petton

Quillien³

et al. 2016

Aquacult. Environ. Interact.

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Mortality of young Pacific oysters Crassostrea gigas associated with the ostreid herpesvirus 1 (OsHV-1) is occurring worldwide. Here, we examined for the first time the effect of salinity on OsHV-1 transmission and disease-related mortality of C. gigas, as well as salinityrelated effects on the pathogen itself. To obtain donors for OsHV-1 transmission, we transferred laboratory-raised oysters to an estuary during a disease outbreak and then back to the laboratory. Oysters that tested OsHV-1 positive were placed in seawater tanks (35 ‰, 21°C). Water from these tanks was used to infect naïve oysters in 2 experimental setups: (1) oysters acclimated or nonacclimated to a salinity of 10, 15, 25 and 35 ‰ and (2) oysters acclimated to a salinity of 25 ‰; the latter were exposed to OsHV-1 water diluted to a salinity of 10 or 25 ‰. The survival of oysters exposed to OsHV-1 water and acclimated to a salinity of 10 ‰ was > 95%, compared to only 43 to 73% survival in oysters acclimated to higher salinities (Expt 1), reflecting differences in the levels of OsHV-1 DNA and viral gene expression (Expts 1 and 2). However, the survival of their nonacclimated counterparts was only 23% (Expt 2), and the levels of OsHV-1 DNA and the expression of 4 viral genes were low (Expt 1). Thus, OsHV-1 may not have been the ultimate cause of mortality in non-acclimated oysters weakened by a salinity shock. It appears that reducing disease risk by means of low salinity is unlikely in the field.

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Section: Introductionmentioning

confidence: 99%

Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1)

Fuhrmann¹,

Petton

Quillien³

et al. 2016

Aquacult. Environ. Interact.

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“…The presence of immune response could also be suggested by the overexpression of imm and tlp genes, as a result of a synergistic effect when both toxic dinoflagellates were present. Studies have suggested that bivalves, and principally bivalve cells of the immune system, can be adversely affected by HAB (Galimary et al, 2008;Lassudrie et al, 2015b). In some cases, a clear modulation of hemato-immunological parameters was observed, especially cellular immune response although most of these studies were performed under controlled laboratory conditions (Ferraz-Mello et al, 2010;Haberkorn et al, 2010;Mello et al, 2013;Lassudrie et al, 2015a).…”

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confidence: 99%

Differential expression of immune response genes in Pacific oyster, Crassostrea gigas spat, fed with dinoflagellates Gymnodinium catenatum and Prorocentrum lima

Kao-Godinez¹,

Romero-Geraldo²,

García-Lagunas³

et al. 2019

LAJAR

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The effects of Gymnodynium catenatum and Prorocentrum lima, dinoflagellate species present in the Gulf of California, on marine bivalves, have demonstrated physiological alterations and changes in gene expression patterns; however, modification effects of the genes involved in immune defense are still poorly understood. This study analyzed the mRNA levels of five genes encoding for the immune defense of oyster Crassostrea gigas spat fed with a control diet (Isochrysis galbana alone) and a combination of the two toxic dinoflagellates. Expression levels of lipopolysaccharide (LPS)-binding protein 1,3-glucan and cavorting genes were higher in oysters exposed to the combined diets in all treatments compared to the non-toxic diet at day three, which was probably related with an activation of the oysters' immediate immune response during the first 24 h. Protein 44 interferon-induced gene expression level was repressed in treatments with the highest dinoflagellate concentration and overexpressed in the diet with equal dinoflagellate concentration. Interaction protein-Toll and immunoglobulin gene transcript levels reached the highest values at day seven in oysters exposed to all combined diets. Then, the immune defense appeared to be activated in oyster spat as a response of toxins and/or extra-cellular compounds produced by the dinoflagellates.

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“…Harmful algae have been shown to temper as well as aggravate the host-pathogen interactions in both adult and juvenile bivalves [18,33,38]. The implications of an elevated larval PO activity are, however, largely unknown as the immunological role of PO is still poorly understood.…”

Section: Discussionmentioning

confidence: 99%

Common European harmful algal blooms affect the viability and innate immune responses of Mytilus edulis larvae

Rijcke

Vandegehuchte

Bussche

et al. 2015

Fish & Shellfish Immunology

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Interaction between toxic dinoflagellate Alexandrium catenella exposure and disease associated with herpesvirus OsHV-1 μVar in Pacific oyster spat Crassostrea gigas

Cited by 25 publications

References 61 publications

Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1)

Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1)

Differential expression of immune response genes in Pacific oyster, Crassostrea gigas spat, fed with dinoflagellates Gymnodinium catenatum and Prorocentrum lima

Common European harmful algal blooms affect the viability and innate immune responses of Mytilus edulis larvae

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