Interaction between Thiamethoxam and Deformed Wing Virus Type A on Wing Characteristics and Expression of Immune and Apoptosis Genes in Apis mellifera

Abstract

Honey bees are economically important insects for crop pollination. They play a significant role as pollinators of wild plants and agricultural crops and produce economical products, such as honey, royal jelly, wax, pollen, propolis, and venom. Despite their ecological and economical importance, the global honey bee population is in decline due to factors including pathogens, parasites, intensive agriculture, and pesticides. Moreover, these factors may be interlinked and exacerbate the loss of honey bees. This study aimed to investigate the interaction between a pesticide, thiamethoxam, and deformed wing virus type A (DWV-A) to honey bees and the effects on survival rate, wing characteristics, and expression of immune and apoptosis genes in Apis mellifera. We described the potential interaction between thiamethoxam and DWV-A on honey bee wing characteristics, DWV-A loads, and the expressions of immune (defensin, abaecin, and hymenoptaecin) and apoptosis genes (buffy, apaf1, caspase3-like, caspase8-like, and caspase9-like). Honey bee larvae were fed with three different thiamethoxam doses (0.001, 1.4, and 14.3 ng/µL of the diet). Then, thiamethoxam-treated white-eyed pupae were injected with 107 copy numbers/honey bee of the DWV-A genome. The interaction between thiamethoxam and DWV-A caused a high mortality rate, crippled wings in newly emerged adult honey bees (100%), and resulted in induced expression of hymenoptaecin gene compared to the control group, while downregulation of caspase8-like, caspase9-like genes compared to the DWV injection group. Therefore, the potential interaction between thiamethoxam and DWV-A might have a deleterious effect on honey bee lifespan. The results from this study could be used as a tool to combat DWV-A infection and mitigate pesticide usage to alleviate the decrease in the honey bee population.

Unraveling the Role of Antimicrobial Peptides in Insects

“…In the bumblebee Bombus terrestris , the level of hymenoptaecin gene expression increased after injection of IAPV [ 204 ]. Similarly, A. mellifera infection with DWV as well as concomitant exposure to DWV type A and a pesticide thiamethoxam resulted in increased expression of the hymenoptaecin gene [ 205 , 206 ]. Mookhploy et al showed that the level of hymenoptaecin and defensin expression increased after injection of DWV into A. mellifera pupae, while abaecin and hymenoptaecin genes were upregulated in newly emerged adult bees [ 207 ].…”