Interaction between the CD45 antigen and phytohemagglutinin. Inhibitory effect on the lectininduced T cell proliferation by anti‐CD45 monoclonal antibody

Bernabéu, Carmelo; Carrera, Ana C.; Landázuri, Manuel O.; Sánchez-Madrid, Francisco

doi:10.1002/eji.1830171012

Cited by 55 publications

(24 citation statements)

References 31 publications

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“…The mAb D3/9 (anti-CD45) [38], FG1/6 (anti-transferrin receptor or CD71) [39] and HCl/l (anti-p150,95 or CDllc) 1351 were generated in our laboratory. The mAb TSIjl1 (anti-LFA-1 or CDlla) and TS1/18 (anti-LFA-I or CD18) have been previously described 1161 and the RRl/l mAb (anti-ICAM-1 or CD54) (61 was kindly provided by Dr T. A.…”

Section: An T Ih Odiesmentioning

confidence: 99%

Phorbol esters induce differentiation of U‐937 human promonocytic cells in the absence of LFA‐1/ICAM‐1‐mediated intercellular adhesion

Cabañas

Sánchez‐Madrid

Aller

et al. 1990

European Journal of Biochemistry

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Intercellular adhesions which occur during the mononuclear phagocyte differentiation are predominantly mediated by the lymphocyte-function-associated antigen-1 (LFA-1) family and the intercellular-adhesion molecule-1 (ICAM-1) which is a ligand for LFA-1. Thus, differentiation of U-937 promonocytic cells induced by phorbol esters occurs concomitantly with intercellular LFA-l/ICAM-1 -dependent cluster formation. Since these homotypic adhesions can be inhibited by monoclonal antibodies (mAb) directed to either LFA-1 or ICAM-1, we have analyzed whether the lack of cell -cell adhesions impairs the differentiation process. Treatment of U-937 cells with the phorbol ester 12-0-tetradecanoylphorbol 13-acetate in the presence of mAb to LFA-1 or ICAM-1 antigens yielded cells free from homotypic adhesions but differentiated as evidenced by their decreased proliferation and enhanced capacity for generation of superoxide anion. In addition, expression of the C D l l c antigen was increased, whereas the transferrin receptor disappeared from the cell surface. Vimentin gene transcription was also greatly augmented as opposed to a clear diminution in the levels of c-myc and ornithine decarboxylase transcripts. These results clearly demonstrate that phorbol esters can induce differentiation of monocytic cells independently of cell -cell adhesion.Intercellular adhesions play an important role in many immunological functions. Many of the adhesion phenoma of leucocytes are mediated by the lymphocyte-function-associated antigen 1 (LFA-1) family and the intercellular-adhesion molecule 1 (ICAM-1, CD54) molecule, which is a ligand for LFA-1 [I -61. These molecules are involved in antigen presentation [2, 7, 81, T cell effector functions [9-121, cooperation between B and T lymphocytes [I31 and chemotaxis of myeloid cells [I, 14, 151. The LFA-1 family of antigens is composed of three structurally related glycoproteins, LFA-1 (CD1 la), Mol (CDllb) and p150,95 (CDllc) [16]. These three antigens are noncovalently linked a : j heterodimers which have distinct a subunits of 170, 165 and 150 kDa, respectively, and share a common j subunit of 95 kDa (CD18) [16,17].In mononuclear phagocytes, the regulation of cell -cell adhesion is an important aspect of their function and development. Thus, monocytes initiate diapedesis by adherence to the endothelium. This adhesion to endothelial cells allows the monocytes to exit from blood vessels by passing between adjacent endothelial cells. At the same time that these adhesion processes take place, monocytes maturate into tissue macrophages. Also, upon antigen challenge, there is a massive infiltration of macrophages showing homotypic (macrophage -macrophage) and heterotypic (macrophage -T-cell) interactions. These cellular associations can lead to granuloma Several markers for monocytic differentiation of myeloid cell lines have been used, such as an arrest in their proliferative capacity [23 -25, 281, variations in the levels of specific gene transcripts [26, 29 -331, changes in the expression of specific...

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Section: An T Ih Odiesmentioning

confidence: 99%

Phorbol esters induce differentiation of U‐937 human promonocytic cells in the absence of LFA‐1/ICAM‐1‐mediated intercellular adhesion

Cabañas

Sánchez‐Madrid

Aller

et al. 1990

European Journal of Biochemistry

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“…The D3/9 and RP1/ll MAb are specific for conventional CD45 and CD45R, respectively [13,15]. MC5/2 MAb, directed against an epitope shared by the three CD45 polypeptides of largest size, displays identical reactivity to that of PD7/26/16 MAb, which originally defined such specificity [13,16].…”

Section: Monoclonal Antibodiesmentioning

confidence: 99%

Biochemical and antigenic characterization of CD45 polypeptides expressed on plasma membrane and internal granules of human neutrophils

Pulido¹,

Lacal²,

Mollinedo³

et al. 1989

FEBS Letters

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The expression of CD45 polypeptides, a phosphotyrosine phosphatase complex specific of leukocytes, has been investigated in both resting and activated neutrophils by using anti-CD45 monoelonal antibodies (MAb) which specifically recognize different polypeptide components of the CIM5 molecular complex. Polypeptides of 180 and 130-150 kDa were equally precipitated by either a conventional CD45 MAb recognizing an antigenic determinant shared by the four CD45 glycoproteins (220, 205, 190 and 180 kDa) or by the anti-180 kDa UCHLI MAb. These polypeptides were overexpressed on neutrophil plasma membranes after degranulatory stimulation. Conversely, neither the anti-220 kDa CD45R nor anti-220/205/190 kDa MAb reacted with CD45 molecules from resting or activated neutrophils. Furthermore, permeabilization analysis and comparative immunoprecipitation studies with different anti-CD45 MAb from fractions enriched in various neutrophil granules revealed that CD45 polypeptides (180 and 130-150 kDa) from internal granules are antigenic and biochemically identical to those expressed on plasma membrane.

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“…Plant lectins such as phytohemagglutinin (PHA) or Con A bind to the T cell receptor (TCR)/CD3 complex (17,18), as well as to other, non-antigen-specific cell surface structures such as CD2 (19) and CD45 (20). In normal T cells, t h e net effect of this multivalent binding by mitogen is activation and enhanced proliferative responses.…”

mentioning

confidence: 99%

Enhanced CD3‐mediated T lymphocyte proliferation in patients with systemic lupus erythematosus

Stekman¹,

Blasini²,

Leon‐Ponte³

et al. 1991

Arthritis & Rheumatism

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Nonfractionated peripheral blood lymphocytes from patients with systemic lupus erythematosus (SLE) showed enhanced proliferative responses when stimulated via the CD3 pathway. In contrast, proliferative responses induced by phytohemagglutinin were diminished in SLE patients. Levels of CD3-induced interleukin-2 production and interleukin-2 receptor expression were comparable with normal levels. Highly purified T cells also showed augmented CD3 responses, but only in the presence of phorbol myristate acetate or a combination of phorbol myristate acetate plus calcium ionophore A23187, and not with calcium ionophore alone. The data suggest integrity of the T cell receptorKD3 pathway for T cell activation in patients with SLE, as examined in cultures stimulated with specific anti-CD3 monoclonal antibodies rather than with multivalent lectins. An increased response via the CD3 complex could contribute to the autoimmune activity in human SLE.

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Interaction between the CD45 antigen and phytohemagglutinin. Inhibitory effect on the lectininduced T cell proliferation by anti‐CD45 monoclonal antibody

Cited by 55 publications

References 31 publications

Phorbol esters induce differentiation of U‐937 human promonocytic cells in the absence of LFA‐1/ICAM‐1‐mediated intercellular adhesion

Phorbol esters induce differentiation of U‐937 human promonocytic cells in the absence of LFA‐1/ICAM‐1‐mediated intercellular adhesion

Biochemical and antigenic characterization of CD45 polypeptides expressed on plasma membrane and internal granules of human neutrophils

Enhanced CD3‐mediated T lymphocyte proliferation in patients with systemic lupus erythematosus

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