Interaction between RNA1 and the primer precursor in the regulation of ColE1 replication

Lacatena, Rosa M.

doi:10.1016/s0022-2836(83)80125-9

Cited by 62 publications

(31 citation statements)

References 14 publications

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“…In each case, both the antisense and target RNAs assume stable stem and loop structures (11,17,29,37). Genetic analyses of mutant ColEl (9,33) and IncFII (5,6,27) plasmids led to the suggestion that the specificity of the interaction between the antisense and target RNAs resides in the single-stranded loops of the respective molecules. This notion was supported by observations that even single base substitutions in these loops result in significantly altered antisense-target binding rates in vitro (18,30).…”

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confidence: 99%

Mechanism of binding of the antisense and target RNAs involved in the regulation of IncB plasmid replication

1994

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The replication frequency of the IncB miniplasmid pMU720 is dependent upon the expression of the repA gene. Binding of a small, highly structured, antisense RNA (RNA I) to its complementary target in the RepA mRNA (RNA II) inhibits repA expression and thus regulates replication. Analyses of binding of RNA I to RNA II indicated that the reaction consists of three major steps. The first step, initial kissing complex formation, involves base pairing between complementary sequences in the hairpin loops of RNA I and RNA II. The second step is facilitated by interior loop structures in the upper stems of RNA I and RNA II and involves intrastrand melting and interstrand pairing of the upper stem regions to form an extended kissing complex. This complex was shown to be sufficient for inhibition ofrepA expression. The third step involves stabilization of the extended kissing complex by pairing between complementary single-stranded tail regions of RNA I and RNA II. Thus, the final product of RNA I-RNA II binding is not a full duplex between the two molecules.

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confidence: 99%

Mechanism of binding of the antisense and target RNAs involved in the regulation of IncB plasmid replication

1994

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“…It should be pointed out that these conclusions do not necessarily apply to negative initiation control models in general, or even to all inhibitor-dilution systems, merely to the particular, ColEl-like mechanism (9,14,27,33) considered here. Nevertheless, the recent experimental results of Skarstad et al (28), which can only be understood in terms of a very high degree of initiation synchrony within individual cells, greater than 97% for E. coli B/r growing at T = 27 min, argue strongly against stochastic models of this kind for the control of chromosome replication.…”

Section: Resultsmentioning

confidence: 82%

Initiation of chromosome replication in bacteria: analysis of an inhibitor control model

Margalit

Grover

1987

J Bacteriol

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This article contains an analysis of a version of the well-known inhibitor-dilution model for the control of initiation of chromosome replication in bacteria. According to this model, an unstable inhibitor interacts with an initiation primer in a hit-and-destroy fashion to prevent successful initiation; both constituents are presumed to be RNA species that are synthesized constitutively. The model further postulates that the inhibitor interacts cooperatively with the primer, that the inhibitor gene is removed some distance from the origin of replication, and that an eclipse period exists during which the chromosome origin is not able to reinitiate. This unstable-inhibitor version is characterized by four parameters: the inhibitor half-life, the cooperativity index, the location of the inhibitor gene, and the eclipse period; computer simulations are used to study the effect of each of these on the DNA and interdivision time distributions in exponentially growing steady-state cultures. In neither case was any combination of parameter values found that could provide even moderately satisfactory agreement between the simulation results and experimental data. From the examples furnished and the associated discussion, it appears that there are none-that no combination of parameter values exists that can reasonably be expected to produce a significantly better fit than those tested. We conclude that the model in its present form cannot be a valid description of chromosome replication control in bacteria. It is pointed out that this does not necessarily apply to negative initiation control models in general, or even to all inhibitor-dilution systems, merely to the particular ColEl-like mechanism considered here. Nevertheless, recent experimental results, which can only be understood in terms of a very high degree of initiation synchrony within individual cells, offer strong evidence against stochastic models of this kind for the control of chromosome replication.At any fixed temperature, bacteria in steady-state exponential growth have different average generation times, depending on the composition of the culture medium. Since the duration of chromosome replication and the interval between its termination and the subsequent cell division are both essentially constant, the major contribution to this difference must come from the timing of the initiation event itself (18).The initiation of chromosome replication is thus a critical stage in the cell cycle of bacteria, and despite the considerable experimental data available regarding replication at different growth rates, the precise factors responsible for its control remain largely unknown.Originally it was thought that initiation occurs when a fixed ratio is attained between cell mass and the number of origins of replication, independent of the growth rate (5; In a previous article (16), we presented a detailed quantitative analysis of the initiation structure model originally put forward by Sompayrac and Maal0e (29) and were able to delineate the characteristics that ...

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“…RNAI interacts with RNAII to negatively regulate primer formation by inhibiting processing of the preprimer by ribonuclease H (27,52,61). RNAI acts in trans and is the primary determinant of plasmid incompatibility (13,23,51,53). On the basis of the sequence divergence of RNAI, pWQ799 represents a new incompatibility group among those ColE1-related plasmids for which the RNAI sequence has been determined.…”

Section: Resultsmentioning

confidence: 99%

Lateral transfer of rfb genes: a mobilizable ColE1-type plasmid carries the rfbO:54 (O:54 antigen biosynthesis) gene cluster from Salmonella enterica serovar Borreze

Keenleyside

Whitfield

1995

J Bacteriol

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Plasmid pWQ799 is a 6.9-kb plasmid isolated from Salmonella enterica serovar Borreze. Our previous studies have shown that the plasmid contains a functional biosynthetic gene cluster for the expression of the O:54 lipopolysaccharide O-antigen of this serovar. The minimal replicon functions of pWQ799 have been defined, and a comparison with nucleotide and protein databases revealed this replicon to be virtually identical to ColE1. This is the first report of involvement of ColE1-related plasmids in O-antigen expression. The replicon of pWQ799 is predicted to encode two RNA molecules, typical of other ColE1-type plasmids. RNAII, the putative replication primer from pWQ799, shares regions of homology with RNAII from ColE1. RNAI is an antisense regulator of DNA replication in ColE1-related plasmids. The coding region for RNAI from pWQ799 shares no homology with any other known RNAI sequence but is predicted to adopt a secondary structure characteristic of RNAI molecules. pWQ799 may therefore represent a new incompatibility group within this family. pWQ799 also possesses cer, rom, and mob determinants, and these differ minimally from those of ColE1. The plasmid is mobilizable in the presence of either the broad-host-range helper plasmid pRK2013 or the IncI 1 plasmid R64drd86. Mobilization and transfer of pWQ799 to other organisms provides the first defined mechanism for lateral transfer of O-antigen biosynthesis genes in S. enterica and explains both the distribution of related plasmids and coexpression of the O:54 factor with other O-factors in different Salmonella serovars. The base composition of the pWQ799 replicon sequences gives an average percent G؉C value typical of Salmonella spp. In contrast, the percent G؉C value is dramatically lower within rfb O:54 , consistent with the possibility that the cluster was acquired from an organism with a much lower G؉C composition.

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Interaction between RNA1 and the primer precursor in the regulation of ColE1 replication

Cited by 62 publications

References 14 publications

Mechanism of binding of the antisense and target RNAs involved in the regulation of IncB plasmid replication

Mechanism of binding of the antisense and target RNAs involved in the regulation of IncB plasmid replication

Initiation of chromosome replication in bacteria: analysis of an inhibitor control model

Lateral transfer of rfb genes: a mobilizable ColE1-type plasmid carries the rfbO:54 (O:54 antigen biosynthesis) gene cluster from Salmonella enterica serovar Borreze

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