Interaction between reactive oxygen species and nitric oxide in drought-induced abscisic acid synthesis in root tips of wheat seedlings

Zhao, Zhiguang; Chen, Guo-Cang; Chenglie, Zhang

doi:10.1071/pp00143

Cited by 90 publications

(67 citation statements)

References 36 publications

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“…Whether a model such as that described above exists in plants exposed to water stress is an interesting topic. Although it has been shown that dehydration results in significant increases in the activity of superoxide synthase [NAD(P)H oxidase] in the microsomal and cytosolic fractions from rapidly, severely dehydrated root tips of wheat seedlings (20% of fresh weight lost in about 10 min; Zhao et al 2001), it is not clear whether these changes are physiologically related. Furthermore, these experiments also did not exclude the possible involvement of the NAD(P)H-oxidizing activity of peroxidase.…”

Section: Discussionmentioning

confidence: 98%

“…These observations have demonstrated the existence of a plant NADPH oxidase resembling the neutrophil NADPH oxidase. It has been shown that NADPH oxidase is involved in plant growth and development (Papadakis and Roubelakis-Angelakis 1999;Frahry and Schopfer 2001;Schopfer et al 2001), and in plant response to pathogens, elicitors and wounding (Lamb and Dixon 1997;Bolwell et al 1998;Orozco-Ca´rdenas and Ryan 1999;Orozco-Ca´rdenas et al 2001;Sagi and Fluhr 2001), UV light (Rao et al 1996;A.-H.-Mackerness et al 2001), hypoosmotic stress (Cazale´et al 1998Park et al 2000), zinc and copper deficiency or excess (Pinton et al 1994;Quartacci et al 2001), chilling (Shen et al 2000) and drought (Zhao et al 2001), and plays important roles in AOS production and the regulation of defense strategies in plant cells (Levine et al 1994;Lamb and Dixon 1997;A.-H.-Mackerness et al 2001;Orozco-Ca´rdenas et al 2001).…”

Section: Introductionmentioning

confidence: 98%

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Involvement of plasma-membrane NADPH oxidase in abscisic acid- and water stress-induced antioxidant defense in leaves of maize seedlings

Jiang

Zhang

2002

Planta

332

223

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The roles of the plasma-membrane (PM) NADPH oxidase in abscisic acid (ABA)- and water stress-induced antioxidant defense were investigated in leaves of maize ( Zea mays L.) seedlings. Treatment by exogenous ABA (100 micro M ABA) or osmotic stress (-0.7 MPa induced by polyethylene glycol) significantly increased the activity of the PM NADPH oxidase, the production of leaf O(2)(-), the activities of several antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase), and the contents of antioxidant metabolites (ascorbate and reduced glutathione). Pretreatment with three different inhibitors of NADPH oxidase (diphenylene iodonium, imidazole and pyridine) or an inhibitor of ABA biosynthesis (tungstate) reduced the increase in the activity of the PM NADPH oxidase and the production of leaf O(2)(-), and the capacity of antioxidant defense systems mediated by ABA. The inhibitory effects above caused by tungstate were reversed by exogenous ABA. These data indicate that NADPH oxidase is involved in the ABA-induced production of active oxygen species (AOS), and our results depict a minimal chain of events initiated by water stress-induced ABA accumulation, which then triggers the production of AOS by membrane-bound NADPH oxidase, resulting in the induction of antioxidant defense systems against oxidative damage in plants.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 98%

Involvement of plasma-membrane NADPH oxidase in abscisic acid- and water stress-induced antioxidant defense in leaves of maize seedlings

Jiang

Zhang

2002

Planta

332

223

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“…In contrast, H 2 O 2 activates plasma membrane Ca 2ϩ channels and induces stomatal closure in abi1 but not in abi2 (25). Recently, it was suggested that reactive oxygen species might also be involved in the regulation of ABA biosynthesis (26). It is likely that the observed ABA regulation of ABA biosynthesis genes may be mediated by reactive oxygen species.…”

Section: Los6 Encodes An Enzyme Functioning In Aba Biosynthesis-mentioning

confidence: 94%

Regulation of Osmotic Stress-responsive Gene Expression by theLOS6/ABA1 Locus inArabidopsis

Xiong

Lee

Ishitani

et al. 2002

Journal of Biological Chemistry

385

302

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Drought and high salinity induce the expression of many plant genes. To understand the signal transduction mechanisms underlying the activation of these genes, we carried out a genetic screen to isolate Arabidopsis mutants defective in osmotic stress-regulated gene induction. Here we report the isolation, characterization, and cloning of a mutation, los6, which diminished osmotic stress activation of a reporter gene. RNA blot analysis indicates that under osmotic stress the transcript levels for stress-responsive genes such as RD29A, COR15A, KIN1, COR47, RD19, and ADH are lower in los6 plants than in wild type plants. los6 plants were found to have reduced phytohormone abscisic acid (ABA) accumulation and to be allelic to the ABA-deficient mutant, aba1. LOS6/ABA1 encodes a zeaxanthin epoxidase that functions in ABA biosynthesis. Its expression is enhanced by osmotic stress. Furthermore, we found that there exists a positive feedback regulation by ABA on the expression of LOS6/ABA1, which may underscore a quick adaptation strategy for plants under osmotic stress. Similar positive regulation by ABA also exists for other ABA biosynthesis genes AAO3 and LOS5/ ABA3 and in certain genetic backgrounds, NCED3. This feedback regulation by ABA is impaired in the ABAinsensitive mutant abi1 but not in abi2. Moreover, the up-regulation of LOS6/ABA1, LOS5/ABA3, AAO3, and NCED3 by osmotic stress is reduced substantially in ABA-deficient mutants. Transgenic plants overexpressing LOS6/ABA1 showed an increased RD29A-LUC expression under osmotic stress. These results suggest that the level of gene induction by osmotic stress is dependent on the dosage of the zeaxanthin epoxidase enzyme.Osmotic stress resulting from either high salinity or water deficit induces the expression of numerous stress-responsive genes in plants (1-5). Understanding the mechanisms that regulate the expression of these genes is a fundamental issue in basic plant biology and is instrumental for future genetic improvement of plant productivity under abiotic stresses. Considerable information has been accumulated as a result of molecular studies of gene regulation under osmotic stress (1-5). In contrast, genetic analysis of osmotic signal transduction has been very limited. Because the phytohormone abscisic acid (ABA) 1 is known to be involved in plant responses to various environmental stresses, the availability of ABA-deficient mutants (aba) or ABA-insensitive mutants (abi) in Arabidopsis has provided invaluable opportunities to investigate the role of ABA in plant stress responses. Using these mutants, changes in transcript levels of a few stress-responsive genes were analyzed under cold, drought, or salt stress (for reviews, see Refs. 3, 4, and 6). A general consensus resulting from these studies is that low temperature signaling is less influenced by ABA, whereas drought and salt stress signal transduction has both ABA-dependent and ABA-independent pathways (4, 6).We have been using a reporter gene approach to dissect osmotic stress signal transduction ne...

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“…The NO releasing chemical (SNP), NOS inhibitor (LNNA), NO scavenger (PTIO), and ROS eliminator (NAC) (Zhao et al 2001;Wang et al 2006), were purchased from Sigma, NY, USA, and were applied in vivo by injection (Chen et al 1993) into the intercellular space of maize seedlings through the roots incubated in 300 mL Hoagland's solution containing 0.1 mM SNP or 0.2 mM LNNA or 0.2 mM PTIO or 0.05 mM NAC (regenerated once a day and the hydroponics were aerated as much as three times for 20-30 min/time with air pump) with or without the UV-B treatments. Hoagland's solution was used as a control treatment.…”

Section: Ultraviolet-b Treatmentmentioning

confidence: 99%

Cascade transduction and production of the light-nitric oxide signal from shoots to roots in maize seedlings exposed to enhanced UV-B radiation

Zhang

Chen

et al. 2008

Acta Physiol Plant

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The biomasses, rate of apparent nitric oxide (NO)-release, nitric oxide synthase (NOS) activity as well as b-D-endo and exo-glucanase activity of the cell wall were analyzed and determined in the roots of maize seedlings. It was found that rhizospheric treatments of 2-phenyl-4,4,5,5-tetramethlimida-zoline-l-oxyl-3-oxide (PTIO), a NO scavenger, and radiation of enhanced ultraviolet-B (UV-B) to aerial parts of the seedling markedly inhibited the rate of NO release in roots, raised the activity of b-Dendo and exo-glucanase, and increased the biomasses of roots. The patent inhibitor, N-nitro-L-arginine (LNNA), of NOS was unable to inhibit NOS activity and NO generation. Inversely, reactive oxygen species (ROS) eliminator, N-acetyl-cysteine (NAC), stimulated the rate of NO release. There is no relationship between NOS activity and the rate of NO release. The latter showed a positive correlation with nitrate reductase (NR) activity, whereas it showed a negative correlation with the bio-masses and the activity of b-D-endo and exo-glucanase. All results implicated that NO was a by-product generated by NR catalysis, whereas NR activity was sensitively repressed by the systemic signal network (involved in ROS) induced by enhanced UV-B. It indicated that the downstream signal molecule of enhanced UV-B light is probably ROS which decreased NO generation through inhibiting NR activity. The endogenous NO generated by NR catalysis is perhaps such a messenger for restraining b-D-endo and exoglucanase activity that the root growth was retarded.

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Interaction between reactive oxygen species and nitric oxide in drought-induced abscisic acid synthesis in root tips of wheat seedlings

Cited by 90 publications

References 36 publications

Involvement of plasma-membrane NADPH oxidase in abscisic acid- and water stress-induced antioxidant defense in leaves of maize seedlings

Involvement of plasma-membrane NADPH oxidase in abscisic acid- and water stress-induced antioxidant defense in leaves of maize seedlings

Regulation of Osmotic Stress-responsive Gene Expression by theLOS6/ABA1 Locus inArabidopsis

Cascade transduction and production of the light-nitric oxide signal from shoots to roots in maize seedlings exposed to enhanced UV-B radiation

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