Interaction between melamine and bovine serum albumin: Spectroscopic approach and density functional theory

Yan, Hua; Wu, Junyong; Dai, Guoliang; Zhong, Aiguo; Yang, Jianguo; Liang, Huading; Pan, Fan

doi:10.1016/j.molstruc.2009.12.039

Cited by 6 publications

(4 citation statements)

References 27 publications

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“…It is known that melamine can weakly bind to the protein molecule through hydrogen bond interactions. 33 It is likely that such weak interactions are not effective for HSA to replace the melamine from the particle surface. These results further support the conclusion that the anti- 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 aggregation effect is contributed by HSA adsorbed on the particle surface rather than the interactions between HSA and melamine.…”

Section: Resultsmentioning

confidence: 99%

“…However, when melamine was added into the dispersion first, subsequent addition of HSA had little impact on suppressing the aggregation of the Au NPs. It is known that melamine can weakly bind to the protein molecule through hydrogen bond interactions . It is likely that such weak interactions are not effective for HSA to replace the melamine from the particle surface.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Gold Nanoparticle-Based Facile Detection of Human Serum Albumin and Its Application as an INHIBIT Logic Gate

Huang

Wang

Yang

2015

ACS Appl. Mater. Interfaces

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In this work, a facile colorimetric method is developed for quantitative detection of human serum albumin (HSA) based on the antiaggregation effect of gold nanoparticles (Au NPs) in the presence of HSA. The citrate-capped Au NPs undergo a color change from red to blue when melamine is added as a cross-linker to induce the aggregation of the NPs. Such an aggregation is efficiently suppressed upon the adsorption of HSA on the particle surface. This method provides the advantages of simplicity and cost-efficiency for quantitative detection of HSA with a detection limit of ∼1.4 nM by monitoring the colorimetric changes of the Au NPs with UV-vis spectroscopy. In addition, this approach shows good selectivity for HSA over various amino acids, peptides, and proteins and is qualified for detection of HSA in a biological sample. Such an antiaggregation effect can be further extended to fabricate an INHIBIT logic gate by using HSA and melamine as inputs and the color changes of Au NPs as outputs, which may have application potentials in point-of-care medical diagnosis.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Gold Nanoparticle-Based Facile Detection of Human Serum Albumin and Its Application as an INHIBIT Logic Gate

Huang

Wang

Yang

2015

ACS Appl. Mater. Interfaces

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show abstract

“…For example, HA contain carboxyl (-COOH) and phenolic hydroxyl (-OH) functional groups (Hizal and Apak, 2006), and APS can contain carboxyl (-COOH) and sulfate ester (-O-SO 3 − ) groups (Alvarado Quiroz et al, 2006;Ananthi et al, 2010). BSA also contains disulfide bonds (-S-S-), sulfhydryl (-SH), and amino groups (Yan et al, 2010). These negatively charged MOCs or ligand groups could favor binding to 210 Po, directly or after reduction from the prevailing + IV state (Hussain et al, 1995), leading then to enhanced 210 Po uptake.…”

Section: Effect Of Mocs On the Partitioning Of 210 Po On Nanoparticlesmentioning

confidence: 99%

“…2). Functional groups of BSA include disulfide bonds and sulfhydryl groups in BSA (Yan et al, 2010), which can be oxidized under oxic conditions, and which are less abundant in HA. Thus, BSA binding of 210 Po could be enhanced after reduction.…”

Section: Effect Of Mocs On the Partitioning Of 210 Po On Nanoparticlesmentioning

confidence: 99%

Influence of organic matter on the adsorption of 210Pb, 210Po and 7Be and their fractionation on nanoparticles in seawater

Yang

Guo

Chuang

et al. 2015

Earth and Planetary Science Letters

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Editor: J. Lynch-StieglitzKeywords: radionuclide scavenging natural organic matter nanoparticles polonium lead beryllium Improved applications of 210 Po, 210 Pb and 7 Be as geochemical proxies require more detailed understanding of their interactions with particles. Here, laboratory sorption experiments were carried out to examine the adsorption of 210 Po, 210 Pb and 7 Be and their fractionation on inorganic nanoparticles, including SiO , in the presence or absence of macromolecular organic compounds (MOCs) that include humic acids (HA), acid polysaccharides (APS) and proteins (BSA), in natural seawater. Results showed that nanoparticle sorption was not greatly enhanced over that of microparticles as would be expected from their much higher specific surface areas, likely indicating their aggregation in seawater. It was found that synergistic interactions between inorganic nanoparticles, MOCs, and radionuclides determined the sorption, although their adsorption was particle composition-dependent. MOCs enhanced the sorption of selected nuclides on most nanoparticles. On average, in the presence of MOCs, partition coefficients (K c ) of 210 Po, 210 Pb, and 7 Be on nanoparticles increased 2.9-, 5.0-and 5.9-fold, respectively. The effect of MOCs could be explained for 210 Po and 210 Pb from their different log K c values on inorganic nanoparticles. In addition, fractionation effects between 210 Po and 210 Pb (or between 210 Pb and 7 Be) could be quantified from their relative log K c values on end-member sorbent components. Applications of both 210 Po-210 Pb and 7 Be-210 Pb pairs as particle dynamics tracers could be more quantitative when the nature of the organic coatings is taken into account.

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Fluorometric and molecular modeling deciphering the non-covalent interaction between cyromazine and human serum albumin

Zhang

Ding

Liu

et al. 2017

J Incl Phenom Macrocycl Chem

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Interaction between melamine and bovine serum albumin: Spectroscopic approach and density functional theory

Cited by 6 publications

References 27 publications

Gold Nanoparticle-Based Facile Detection of Human Serum Albumin and Its Application as an INHIBIT Logic Gate

Gold Nanoparticle-Based Facile Detection of Human Serum Albumin and Its Application as an INHIBIT Logic Gate

Influence of organic matter on the adsorption of 210Pb, 210Po and 7Be and their fractionation on nanoparticles in seawater

Fluorometric and molecular modeling deciphering the non-covalent interaction between cyromazine and human serum albumin

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