Interaction between Ingested‐Engineered Nanomaterials and the Gastrointestinal Tract:<i>In Vitro</i>Toxicology Aspects

Laloux, Laurie; Polet, Madeleine; Schneider, Yves‐Jacques

doi:10.1002/9783527697724.ch18

Cited by 6 publications

(2 citation statements)

References 115 publications

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“…Human intestinal epithelial cells (namely, Caco-2 and HT29-MTX cell lines) can be used as in vitro models to study the cytotoxic and genotoxic effects of TiO 2 following its ingestion or a simulated digestion procedure [121]. These are less expensive, have an increased reproducibility and their outcome is more rapidly achieved in comparison with in vivo models [122]. Both referred cell lines are derived from a human epithelial colorectal adenocarcinoma.…”

Section: Ingested Tio 2 Cytotoxic and Genotoxic Effects -Potential Im...mentioning

confidence: 99%

Cellular and Molecular Mechanisms of Toxicity of Ingested Titanium Dioxide Nanomaterials

Vieira

Gramacho

Rolo

et al. 2022

Advances in Experimental Medicine and Biology

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An exponential increase in products containing titanium dioxide nanomaterials (TiO2), in agriculture, food and feed industry, lead to increased oral exposure to these nanomaterials (NMs). Thus, the gastrointestinal tract (GIT) emerges as a possible route of exposure that may drive systemic exposure, if the intestinal barrier is surpassed. NMs have been suggested to produce adverse outcomes, such as genotoxic effects, that are associated with increased risk of cancer, leading to a concern for public health. However, to date, the differences in the physicochemical characteristics of the NMs studied and other variables in the test systems have generated contradictory results in the literature. Processes like human digestion may change the NMs characteristics, inducing unexpected toxic effects in the intestine. Using TiO2 as case-study, this chapter provides a review of the works addressing the interactions of NMs with biological systems in the context of intestinal tract and digestion processes, at cellular and molecular level. The knowledge gaps identified suggest that the incorporation of a simulated digestion process for in vitro studies has the potential to improve the model for elucidating key events elicited by these NMs, advancing the nanosafety studies towards the development of an adverse outcome pathway for intestinal effects.

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Section: Ingested Tio 2 Cytotoxic and Genotoxic Effects -Potential Im...mentioning

confidence: 99%

Cellular and Molecular Mechanisms of Toxicity of Ingested Titanium Dioxide Nanomaterials

Vieira

Gramacho

Rolo

et al. 2022

Advances in Experimental Medicine and Biology

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“…3 Although, nano-based agents are presently being extensively utilized in biological and medicinal approaches, there is a momentous concern regarding human 4 and environmental risk 5 upon implications of fabricated nanomaterials. Recent in vivo 6 and in vitro 6,7 investigations have suggested that utilization of some nanoparticles (NPs) may induce serious toxic health impacts and the application of nanomaterials-based medical agents may result in chronic or acute health problems. 8 Thus, along with the development of unique NPs, simultaneous investigation of the impacts of NPs on the biological systems should be carried out.…”

Section: Introductionmentioning

confidence: 99%

<p>Amorphous aggregation of tau in the presence of titanium dioxide nanoparticles: biophysical, computational, and cellular studies</p>

Fardanesh¹,

Zibaie²,

Shariati³

et al. 2019

IJN

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BackgroundNanoparticles (NPs) when injected into the body can reach target tissues like nervous system and interact with tau proteins and neurons. This can trigger conformational changes of tau and may affect NP toxicity.MethodsIn this study, we used several biophysical techniques (extrinsic and intrinsic fluorescence spectroscopy, circular dichroism (CD) spectroscopy, ultraviolet (UV)-visible spectroscopy), transmission electron microscopy (TEM) investigations, molecular docking and molecular dynamics studies, and cellular assays [3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) and flow cytometry) to reveal how structural changes of tau protein can change the cytotoxicity of titanium dioxide (TiO2) NPs against neuron-like cells (SH-SY5Y) cells.ResultsIt was shown that TiO2 NPs result in hydrophilic interactions, secondary and tertiary structural changes, and the formation of amorphous tau aggregates. Conformational changes of tau increased the induced cytotoxicity by TiO2 NPs. These data revealed that the denatured adsorbed protein on the NP surface may enhance NP cytotoxicity.ConclusionTherefore, this study provides useful insights on the NP–protein interactions and discusses how the protein corona can increase cytotoxicity to determine the efficacy of targeted delivery of nanosystems.

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Nanoencapsulation Technology: Boon to Food Packaging Industries

Das

Chaudhari

Dwivedy

et al. 2020

Nanomaterials and Environmental Biotechnology

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Interaction between Ingested‐Engineered Nanomaterials and the Gastrointestinal Tract:In VitroToxicology Aspects

Cited by 6 publications

References 115 publications

Cellular and Molecular Mechanisms of Toxicity of Ingested Titanium Dioxide Nanomaterials

Cellular and Molecular Mechanisms of Toxicity of Ingested Titanium Dioxide Nanomaterials

<p>Amorphous aggregation of tau in the presence of titanium dioxide nanoparticles: biophysical, computational, and cellular studies</p>

Nanoencapsulation Technology: Boon to Food Packaging Industries

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