Interaction between hypericin and hemoglobin

Vardapetyan, Hrachik; Martirosyan, Alina; Tiratsuyan, S. G.; Hovhannisyan, A.

doi:10.1016/j.jphotobiol.2010.06.011

Cited by 15 publications

(6 citation statements)

References 19 publications

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“…To measure the activity of Compound 21 on the protein and membrane structures in a nuclear-free system, we performed a phototoxicity test (Photo-RBC test) which combines the analysis of photohemolysis and hemoglobin oxidation [ 19 , 20 ]. The results of the test showed that the compound in concentrations of 0.4 and 1 μM does not induces hemolysis in the dark ( Figure 8 ).…”

Section: Resultsmentioning

confidence: 99%

Dark and Photoinduced Cytotoxic Activity of the New Chlorophyll-a Derivatives with Oligoethylene Glycol Substituents on the Periphery of Their Macrocycles

Pylina

Shadrin

Шевченко

et al. 2017

IJMS

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In the present work, we investigated the dark and photoinduced cytotoxic activity of the new chlorophyll-a derivatives which contain the substituents of oligoethylene glycol on the periphery of their macrocycles. These compounds were tested using human cell lines to estimate their potential as photosensitizers for photodynamic therapy of cancer. It was shown that all the tested compounds have expressed photoinduced cytotoxic activity in vitro. Detailed study of the biological activity of one of the most perspective compound in this series—pyropheophorbide-a 17-diethylene glycol ester (Compound 21) was performed. This new compound is characterized by lower dark cytotoxicity and higher photoinduced cytotoxicity than previously described in a similar compound (DH-I-180-3) and clinically used PhotolonTM. Using fluorescent microscopy, it was shown that Compound 21 quickly penetrates the cells. Analysis of caspase-3 activity indicated an apoptosis induction 40 min after exposure to red light (λ = 660 nm). The induction of DNA damages and apoptosis was shown using Comet assay. The results of expression analysis of the stress-response genes indicate an activation of the genes which control the cell cycle and detoxification of the free radicals after an exposure of HeLa cells to Compound 21 and to red light. High photodynamic activity of this compound and the ability to oxidize biomolecules was demonstrated on nuclear-free mice erythrocytes. In addition, it was shown that Compound 21 is effectively activated with low energy 700 nm light, which can penetrate deep into the tissue. Thus, Compound 21 is a prospective substance for development of the new drugs for photodynamic therapy of cancer.

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Section: Resultsmentioning

confidence: 99%

Dark and Photoinduced Cytotoxic Activity of the New Chlorophyll-a Derivatives with Oligoethylene Glycol Substituents on the Periphery of Their Macrocycles

Pylina

Shadrin

Шевченко

et al. 2017

IJMS

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“…That indicates an absence of Zn-1 interactions with the hemoglobin. [24] Similar values of absorption at 560, 577 and 630 nm showed that the reference and experimental samples have the same contents of native and oxidized hemoglobin forms. So metHb/oxyHb and ferrylHb/oxyHb relations were 0.569±0.026 and 0.138±0.001 in intact erythrocytes, and 0.491±0.033 and 0.140±0.004 respectively in the cells treated with the porphyrinate.…”

Section: Expression Of Stress Responsive Genes Under Zn-1mentioning

confidence: 71%

“…Shifting of Soret band (407 nm) was determined and the optical absorption value was measured. [24] Ratios of different hemoglobin forms (oxyHb, metHb and ferrylHb) were also calculated using extinction coefficients. [25] …”

Section: Assessment Of Zn-1 Ability To Oxidize Hemoglobinmentioning

confidence: 99%

Cytotoxicity of New Metal Porphyrinates Based on Chlorophyll a Derivatives

Pylina¹,

Shadrin²,

Шевченко³

et al. 2017

MHC

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“…7,8 Most of the clinical features of Hyp are related to its low systemic toxicity, high tumor selectivity, clearance rate and phototoxicity. 9 Interaction of the excited Hyp with surrounding molecules results in two types of photooxidative reactions, which can both take place in the photoactivation of Hyp. One of the reactions involves electron or hydrogen atom transfer, leading to the radical form of the photosensitizer, which can react with oxygen to form reactive oxygen species, such as peroxides, superoxide and hydroxyl radicals.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence Detection of DNA Based on Non-covalent π-π Stacking Interaction between 1-Pyrenebutanoic Acid and Hypericin

Zhang

Kong

2016

ANAL. SCI.

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Herein, we applied hypericin (Hyp) for the detection of DNA on streptavidin-functionalized magnetic beads (SA-MB) through non-covalent π-π stacking interaction between 1-pyrenebutanoic acid (PBA) and Hyp. Briefly, the target DNA (tDNA) was hybridized to morpholino oligonucleotide (MO) that was immobilized on the surface of SA-MB. PBA was incorporated to the backbone of tDNA by means of phosphate-zirconium-carboxylate coordination reaction. Then, fluorescent Hyp was linked to PBA via π-π stacking interaction. Under the optimal conditions, this biosensor displayed a good linear relationship between the fluorescence intensity and the tDNA concentrations in the range from 1 to 100 nM with a low detection limit of 2.9 nM. The mismatch type in a specific DNA sequence can also be efficiently discriminated. Compared with some other reports, this method is more convenient, and has great potential for practical applications.

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Interaction between hypericin and hemoglobin

Cited by 15 publications

References 19 publications

Dark and Photoinduced Cytotoxic Activity of the New Chlorophyll-a Derivatives with Oligoethylene Glycol Substituents on the Periphery of Their Macrocycles

Dark and Photoinduced Cytotoxic Activity of the New Chlorophyll-a Derivatives with Oligoethylene Glycol Substituents on the Periphery of Their Macrocycles

Cytotoxicity of New Metal Porphyrinates Based on Chlorophyll a Derivatives

Fluorescence Detection of DNA Based on Non-covalent π-π Stacking Interaction between 1-Pyrenebutanoic Acid and Hypericin

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