Interaction between Host and Microbes in the Semen of Patients with Idiopathic Nonobstructive Azoospermia

Chen, Peigen; Li, Yanqing; Zhu, Xinning; Ma, Menghui; Chen, Haicheng; He, Jun; Liang, Xiaoyan; Liu, Guihua; Yang, Xing

doi:10.1128/spectrum.04365-22

Cited by 7 publications

(7 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They set up three negative controls to exclude potential contaminants from air, tabletop and reagents. Contaminants in 16s RNA sequences were removed using the Decontam R package [9]. In agreement with Campbell et al, alpha diversity was similar between the groups; however, they found significant differences between groups at genera level; Ruegeria and Donghicola dominated the NOA group.…”

Section: Molina Et Al Assessed the V3-4 Hypervariable Region Of 16s R...supporting

confidence: 79%

“…The culture or PCR-based methods used to inform this opinion are fundamentally flawed due to their inability to culture all bacteria [52]. More recently, advanced next-generation sequencing techniques (NGS) have allowed analysis of the seminal microbiome and identification of its unique composition [8][9][10][11] (Table 1). The exact origins of the bacteria within human seminal fluid remain unclear, and it is not known whether they represent transient colonisation or a static resident flora.…”

Section: The Human Seminal Microbiomementioning

confidence: 99%

“…Bioinformatic programmes then compare these 'reads' to the reference genome (Figure 1). For NGS of the microbiome 16S RNA with 6 hypervariable regions, (V1-6) is used as the 16s RNA gene present in all bacteria with regional sequence variation to characterise species [8][9][10][11].…”

Section: Next-generation Sequencing Of the Human Seminal Microbiomementioning

confidence: 99%

“…At genera level, Escherichia, Shigella, Sneathia and Raoutella differed significantly between groups [64]. Chen et al also investigated the microbiomes of men with NOA (n = 30) and normal controls (n = 30) via NGS of the V3-V4 16S RNA hypervariable region [9]. The hands and penis were washed thoroughly with warm soapy water and 75% alcohol for disinfection prior to collection.…”

Section: Molina Et Al Assessed the V3-4 Hypervariable Region Of 16s R...mentioning

confidence: 99%

“…Dysbiosis of the female reproductive tract has been associated with adverse pregnancy outcomes and impaired response to fertility treatment [6,7]. Some authors have described an association between the presence of certain micro-organisms and specific aberrations in seminal parameters [8][9][10][11]. Though, as in other areas of fertility research, the male reproductive tract microbiota has been relatively neglected [12].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Next-Generation Sequencing to Elucidate the Semen Microbiome in Male Reproductive Disorders

Davies,

Minhas,

Jayasena

2023

Medicina

View full text Add to dashboard Cite

Mean sperm counts are declining at an accelerated rate and infertility is increasingly becoming a public health concern. It is now understood that human semen, previously considered to be sterile, harbours its own specific microbiome. Via activated leucocytes and the generation of reactive oxygen species, bacteria have the capability of evoking an immune response which may lead to sperm damage. Men with infertility have higher rates of both reactive oxygen species and sperm DNA damage. Due to the lack of sensitivity of routine culture and PCR-based methods, next-generation sequencing technology is being employed to characterise the seminal microbiome. There is a mounting body of studies that share a number of similarities but also a great range of conflicting findings. A lack of stringent decontamination procedures, small sample sizes and heterogeneity in other aspects of methodology makes it difficult to draw firm conclusions from these studies. However, various themes have emerged and evidence of highly conserved clusters of common bacteria can be seen. Depletion or over-representation of specific bacteria may be associated with aberrations in traditional and functional seminal parameters. Currently, the evidence is too limited to inform clinical practice and larger studies are needed.

show abstract

Section: Molina Et Al Assessed the V3-4 Hypervariable Region Of 16s R...supporting

confidence: 79%

Section: The Human Seminal Microbiomementioning

confidence: 99%

Section: Next-generation Sequencing Of the Human Seminal Microbiomementioning

confidence: 99%

Section: Molina Et Al Assessed the V3-4 Hypervariable Region Of 16s R...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Next-Generation Sequencing to Elucidate the Semen Microbiome in Male Reproductive Disorders

Davies,

Minhas,

Jayasena

2023

Medicina

View full text Add to dashboard Cite

show abstract

Microbial composition of goat buck's ejaculates is modified by the process of preparing and storing refrigerated semen doses

et al. 2023

View full text Add to dashboard Cite

Exploring the Microbiome in Human Reproductive Tract: High-Throughput Methods for the Taxonomic Characterization of Microorganisms

Salas-Espejo,

Terrón-Camero,

Ruiz

et al. 2023

Semin Reprod Med

View full text Add to dashboard Cite

Microorganisms are important due to their widespread presence and multifaceted roles across various domains of life, ecology, and industries. In humans, they underlie the proper functioning of multiple systems crucial to well-being, including immunological and metabolic functions. Emerging research addressing the presence and roles of microorganisms within human reproduction is increasingly relevant. Studies implementing new methodologies (e.g., to investigate vaginal, uterine, and semen microenvironments) can now provide relevant insights into fertility, reproductive health, or pregnancy outcomes. In that sense, cutting-edge sequencing techniques, as well as others such as meta-metabolomics, culturomics, and meta-proteomics, are becoming more popular and accessible worldwide, allowing the characterization of microbiomes at unprecedented resolution. However, they frequently involve rather complex laboratory protocols and bioinformatics analyses, for which researchers may lack the required expertise. A suitable pipeline would successfully enable both taxonomic classification and functional profiling of the microbiome, providing easy-to-understand biological interpretations. However, the selection of an appropriate methodology would be crucial, as it directly impacts the reproducibility, accuracy, and quality of the results and observations. This review focuses on the different current microbiome-related techniques in the context of human reproduction, encompassing niches like vagina, endometrium, and seminal fluid. The most standard and reliable methods are 16S rRNA gene sequencing, metagenomics, and meta-transcriptomics, together with complementary approaches including meta-proteomics, meta-metabolomics, and culturomics. Finally, we also offer case examples and general recommendations about the most appropriate methods and workflows and discuss strengths and shortcomings for each technique.

show abstract

Interaction between Host and Microbes in the Semen of Patients with Idiopathic Nonobstructive Azoospermia

Abstract: Idiopathic nonobstructive azoospermia is one of the great challenges in assisted reproductive therapy. Although microdissection testicular sperm extraction technology is currently available, many men with iNOA still face the problem of poor sperm retrieval and poor sperm quality.

Cited by 7 publications

References 37 publications

Next-Generation Sequencing to Elucidate the Semen Microbiome in Male Reproductive Disorders

Next-Generation Sequencing to Elucidate the Semen Microbiome in Male Reproductive Disorders

Microbial composition of goat buck's ejaculates is modified by the process of preparing and storing refrigerated semen doses

Exploring the Microbiome in Human Reproductive Tract: High-Throughput Methods for the Taxonomic Characterization of Microorganisms

Contact Info

Product

Resources

About