Interaction Between Grasses and &lt;em&gt;Epichloe&lt;/em&gt; Endophytes and Its Significance to Biotic and Abiotic Stress Tolerance and the Rhi-Zosphere

Lee, Kendall; Missaoui, Ali; Mahmud, Kishan; Presley, Holly; Lonnee, Marin

doi:10.20944/preprints202109.0310.v1

Cited by 13 publications

(1 citation statement)

References 153 publications

(205 reference statements)

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“…Previous studies revealed that rhizosphere microbial communities are susceptible to changes in environmental and plant varieties (Lee et al, 2021;Munoz et al, 2021). However, it is still unclear how tea varieties and soil characteristics affect the structure and functions in rhizosphere bacterial and fungal communities, and the information on beneficial microbial groups is largely lacking.…”

Section: Introductionmentioning

confidence: 99%

Diversity in rhizospheric microbial communities in tea varieties at different locations and tapping potential beneficial microorganisms

Zhang

Fan

et al. 2022

Front. Microbiol.

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Soil microenvironments and plant varieties could largely affect rhizosphere microbial community structure and functions. However, their specific effects on the tea rhizosphere microbial community are yet not clear. Beneficial microorganisms are important groups of microbial communities that hold ecological functionalities by playing critical roles in plant disease resistance, and environmental stress tolerance. Longjing43 and Zhongcha108 are two widely planted tea varieties in China. Although Zhongcha108 shows higher disease resistance than Longjing43, the potential role of beneficial tea rhizosphere microbes in disease resistance is largely unknown. In this study, the structure and function of rhizosphere microbial communities of these two tea varieties were compared by using the Illumina MiSeq sequencing (16S rRNA gene and ITS) technologies. Rhizosphere soil was collected from four independent tea gardens distributed at two locations in Hangzhou and Shengzhou cities in eastern China, Longjing43 and Zhongcha108 are planted at both locations in separate gardens. Significant differences in soil physicochemical properties as demonstrated by ANOVA and PCA, and distinct rhizosphere microbial communities by multiple-biotech analyses (PCoA, LEfSe, Co-occurrence network analyses) between both locations and tea varieties (p < 0.01) were found. Functions of bacteria were annotated by the FAPROTAX database, and a higher abundance of Nitrososphaeraceae relating to soil ecological function was found in rhizosphere soil in Hangzhou. LDA effect size showed that the abundance of arbuscular mycorrhizal fungi (AMF) was higher in Zhongcha108 than that in Longjing43. Field experiments further confirmed that the colonization rate of AMF was higher in Zhongcha108. This finding testified that AMF could be the major beneficial tea rhizosphere microbes that potentially function in enhanced disease resistance. Overall, our results confirmed that locations affected the microbial community greater than that of tea varieties, and fungi might be more sensitive to the change in microenvironments. Furthermore, we found several beneficial microorganisms, which are of great significance in improving the ecological environment of tea gardens and the disease resistance of tea plants. These beneficial microbial communities may also help to further reveal the mechanism of disease resistance in tea and potentially be useful for mitigating climate change-associated challenges to tea gardens in the future.

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Section: Introductionmentioning

confidence: 99%

Diversity in rhizospheric microbial communities in tea varieties at different locations and tapping potential beneficial microorganisms

Zhang

Fan

et al. 2022

Front. Microbiol.

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Effects of Epichloë Endophyte and Transgenerational Effects on Physiology of Achnatherum inebrians under Drought Stress

et al. 2022

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The present study explored the effects of an Epichloë endophyte on growth and physiology parameters of drunken horse grass (DHG, Achnatherum inebrians) under four different soil water content. The possible transgenerational effects (TGE) on the above-mentioned indicators were examined. DHG plants with (EI) and without (EF) this Epichloë endophyte, grown from seed of plants from the same seed line, were used. The seeds had originated in the relatively dry site at Yuzhong [YZ(D)], and also used were seed of plants from this original seed-line grown at the relatively wet site Xiahe [XH(W)]. The growth, photosynthesis, phytohormones, and elements were measured. This study showed that the endophyte increased the aboveground biomass and chlorophyll content, with the increasing of photosynthetic parameters. The presence of endophyte also significantly promoted abscisic acid and indolE−3-acetic acid content but decreased the cytokinin content. The nitrogen and phosphorus content of EI plants was significantly higher than that of EF plants, but the endophyte decreased ratios of C:N and C:P at drought condition. In addition, TGE were present, affecting host growth and the above-mentioned parameters, and which indicated that the plants grown from the seeds in YZ(D) site are more competitive than those in the XH(W) site under water deficiency conditions.

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Accumulation of Alkaloids in Different Tall Fescue KY31 Clones Harboring the Common Toxic Epichloë coenophiala Endophyte under Field Conditions

et al. 2023

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Tall fescue (Lolium arundinaceum) is a highly adaptable forage, pasture and turf grass that is grown on over 14 M ha in the eastern half of the United States and in other temperate regions of the world. A significant factor in adaptability, productivity and stand persistence is in part due to the presence of an intercellular, seed-transmissible, endophytic fungus, Epichloë coenophiala. Epichloë endophytes have been shown to produce a number of alkaloid compounds only in planta, some that are beneficial in repelling insects, while others are toxic to animals. The goal of this work was to monitor the level of the ergot and loline (classified as pyrrolizidine) alkaloid accumulation in individual plants to determine the plant genotype contribution to alkaloid concentrations. The experimental design consisted of sixteen tall fescue KY31 clones in a space-planted, replicated trial over three years. Our results demonstrated that while changes in the alkaloid concentrations for each plant/endophyte genotype were observed over the three years, the overall alkaloid levels remained relatively constant when compared to other plant/endophyte genotypes combinations in the field. Additionally, overall levels of the ergot and loline alkaloid accumulation did not vary in the same way over the three years. Since the E. coenophiala endophyte genotype was the same across all clones, our results indicate that it is the plant genotype that is responsible for determining alkaloid levels in each plant, and suggest that the signal(s) from the plant to the endophyte may not be the same for ergot and loline alkaloid production.

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Interaction Between Grasses and <em>Epichloe</em> Endophytes and Its Significance to Biotic and Abiotic Stress Tolerance and the Rhi-Zosphere

Cited by 13 publications

References 153 publications

Diversity in rhizospheric microbial communities in tea varieties at different locations and tapping potential beneficial microorganisms

Diversity in rhizospheric microbial communities in tea varieties at different locations and tapping potential beneficial microorganisms

Effects of Epichloë Endophyte and Transgenerational Effects on Physiology of Achnatherum inebrians under Drought Stress

Accumulation of Alkaloids in Different Tall Fescue KY31 Clones Harboring the Common Toxic Epichloë coenophiala Endophyte under Field Conditions

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