Interaction between gene repair and mobile elements-induced activity systems after low-dose irradiation

Abstract

Purpose: To analyze the role of mus-genes repair in system activation P-elements in Drosophila melanogaster induced by a chronic exposure to low doses. Materials and methods: The materials were dysgenic individuals of Drosophila melanogaster with mutations in repair genes (mus101, mus205, mus304, mus308, mus309) and simultaneous transposition of mobile P-elements. The animals were exposed to a chronic irradiation in low doses (0.42 mGy/h). The reaction of animals was analyzed by the DNA damage rate in somatic cells ('Comet assay'), level of dominant lethal mutations, fecundity, and survival rate. Results: The combined action of the systems of post-replication, recombination repair (mus205, mus304), repair of DNA double-stranded breaks (mus304), and of the transposition activity of P-elements after a chronic irradiation in low doses was identified according to every study parameter. The other repair systems and their genes (mus101, mus308, and mus309) responded to action of only one factor (irradiation or mobile elements transposition). Conclusion: The obtained data significantly contribute to the knowledge on a new reaction of the mechanisms of organisms to a chronic irradiation in low doses. ARTICLE HISTORY

Low Dose Gamma Radiation Induced Chromosomal Aberrations For the Management of the Green Vegetable Stink Bug, Nezara viridula (Hemiptera: Pentatomidae)

“…The biological efficiency of low dose irradiation could be higher than the exposure to acute irradiation (Grosovsky, 1999;Ma et al, 2010;Yushkova and Zainullin, 2016). The main effect of low doses is radiation-induced genetic instability which lasts during cell divisions after the cell has been irradiated (Holmberg et al, 1998;McIlrath et al, 2003).…”

Involvement of DNA Repair Genes and System of Radiation-Induced Activation of Transposons in Formation of Transgenerational Effects

“…This is confirmed not only by numerous studies of other authors, but by our own experimental facts. Both irradiation and mutations in stress-response genes have been shown to affect the effects of TE transpositions under dysgenic conditions (Yushkova and Zainullin, 2016). This is reflected in an increase of sterility, the frequency of locus-specific mutability, recessive and dominant lethal mutations (Margulies et al, 1986(Margulies et al, , 1987Yushkova and Zainullin, 2014).…”

“…DNA damage in neuroblasts and midgut cells of F 1 /F 2 third instar larvae was assessed using the Comet assay (Yushkova and Zainullin, 2016). The cell suspension was obtained by treating somatic tissues to a collagenase solution (type IV, 0.5 mg/ml PBS) at 37 • C. The obtained cells of the nerve ganglia and midgut (10 µl) were mixed with 0.5% low melting point agarose (100 µl) and applied on glass slides coated with a layer of 1% normal melting point agarose.…”

“…This article is a continuation of the study of the mechanisms of interaction between the systems of transposition of TEs as components of epigenetic regulation and DNA repair in the cell under conditions of chronic radiation exposure (Yushkova and Zainullin, 2016). We have established that there is a specificity of turning-on of certain repair processes and their genes as a result of a particular effect (transposition of P elements or irradiation).…”

“…TEs are a movable part of the genome and DNA fragments that can move around the genome and destabilize it (Finnegan, 1989;Kaufman and Rio, 1992;Jurka et al, 2007;McCullers and Steiniger, 2017). To date, a connection has been established between P elements and repair processes which is described details in Banga et al (1991) and Yushkova and Zainullin (2016). The P transposon acting as a "model" intracellular factor is capable of destabilizing the genome only in a certain genetic P-M system of hybrid dysgenesis (HD) (Kidwell et al, 1977).…”