Interaction between fibronectin and purified staphylococcal clumping factor

Chhatwal, G.S.

doi:10.1016/0378-1097(87)90217-5

FEMS Microbiology Letters

1987

DOI: 10.1016/0378-1097(87)90217-5

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Interaction between fibronectin and purified staphylococcal clumping factor

G.S. Chhatwal

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1992

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Cited by 2 publications

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“…However, in 1954, Duthie (7) suggested that coagulase and clumping factor were separate entities. Since this work was published, there have been several reports claiming to have identified, and in some cases to have purified, clumping factor (6,22,37,39). Recently, Boden and Flock (3,4) showed that coagulase could bind to fibrinogen as well as to prothrombin and that a proportion of the normally extracellular protein was tightly associated with the cell surface.…”

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confidence: 96%

Genetic evidence that bound coagulase of Staphylococcus aureus is not clumping factor

1992

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Staphylococcus aureus Newman cells carry a surface receptor for fibrinogen called clumping factor. The bacteria also express coagulase, an extracellular protein that binds to prothrombin to form a complex with thrombinlike activity which coverts fibrinogen to fibrin. We have confirmed a recent report (M. K. Bodén and J.-I. Flock, Infect. Immun. 57:2358-2363, 1989) that coagulase can bind to fibrinogen as well as to prothrombin and also that a fraction of coagulase is firmly attached to the cell. A mutant with a deletion in the chromosomal coa gene was isolated by allelic replacement. Allelic replacement either was directly selected by electrotransformation of S. aureus R3N4220 with a nonreplicating suicide plasmid, pCOA18, carrying the delta coa::Tcr mutation or occurred after transduction of the integrated pCOA18 plasmid. The coa mutant was completely devoid of coagulase activity but interacted both with soluble fibrinogen and with solid-phase fibrinogen with the same avidity as the parental strain. This strongly suggests that the bound form of coagulase is not clumping factor and is not responsible for the adherence of S. aureus Newman to solid-phase fibrinogen. The fibrinogen binding determinant of coagulase was located in the C terminus of the protein, by analyzing truncated fusion proteins, in contrast to the prothrombin-binding region which was located in the N terminus.

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confidence: 96%

Genetic evidence that bound coagulase of Staphylococcus aureus is not clumping factor

1992

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“…bacterial whole cells by fibronectin shown by Chhatwal et al (2). In order to avoid any nonspecific binding of lgG to staphylococcal protein A, furthermore, the Fc regions were removed from all the antibody preparations in the ELISA forfibrinogen-binding activities.…”

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confidence: 99%

“…Considering that the staphylococcal clumping factor directly binds to fibrinogen molecules (1)(2)(3)(4)(5)8), absence of such a singificant correlation between expression of these two activities may suggest the following possibilities: (a) the clumping may be a phenomenon in which several factors including the fibrinogen-binding material(s) participate;…”

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confidence: 99%

Expression of Clumping and Fibrinogen‐Binding Activities of Staphylococcus aureus at Various Growth Stages

Yonemasu¹,

Sasaki²,

Ohmae³

et al. 1991

Microbiology and Immunology

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Clumping and fibrinogen-binding activities of 4 Staphylococcus aureus strains (Cowan I, Newman D2C, Wood 46 and NCTC 5655) were assayed with a semiquantitative clumping test and an enzyme-linked immunosorbent assay (ELISA), respectively.Distinct positive clumping was detected with whole cells of the 3 strains except Wood 46. Amounts of fibrinogen required for a definite clumping depended greatly on strains as well as on their growth phases.On the other hand, fibrinogen-binding activities were detected both in culture supernatants and in cell lysates of all the 4 strains, and the levels were rather comparable with one another and relatively steady through their growth cycles. No significant correlation was thus found among expression behavior of clumping and fibrinogen-binding activities.

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Interaction between fibronectin and purified staphylococcal clumping factor

Cited by 2 publications

References 8 publications

Genetic evidence that bound coagulase of Staphylococcus aureus is not clumping factor

Genetic evidence that bound coagulase of Staphylococcus aureus is not clumping factor

Expression of Clumping and Fibrinogen‐Binding Activities of Staphylococcus aureus at Various Growth Stages

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