Interaction between carbon dots from folic acid and their cellular receptor: a qualitative physicochemical approach

Adhel, Erika; Ha-Duong, Nguyêt-Thanh; Vu, Thi Huyen; Taverna, Dario; Ammar, Souad; SERRADJI, Nawal

doi:10.1039/d3cp01277h

Cited by 2 publications

(1 citation statement)

References 56 publications

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“…The selection of these molecules was based on their ability to interact with a broad spectrum of proteins, which significantly influences the composition of the protein corona surrounding NPs. For example B complex components can interact with a wide range of proteins including albumin 25,26 , hemoglobin 25 , myoglobin 27 , pantothenate permease 28 , acyl carrier protein 29 , lactoferrin 30 , prion 31 , β-amyloid precursor 32 , and niacin-responsive repressor 33 . Additionally, to assess the collective effects of these molecules, we analyzed two representative "molecular sauces."…”

Section: Protein Corona and Small Molecules Enable Deep Profiling Of ...mentioning

confidence: 99%

Deep Plasma Proteome Profiling by Modulating Single Nanoparticle Protein Corona with Small Molecules

Ashkarran,

Gharibi,

Modaresi

et al. 2024

Preprint

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The protein corona, a dynamic biomolecular layer that forms on nanoparticle (NP) surfaces upon exposure to biological fluids is emerging as a valuable diagnostic tool for improving plasma proteome coverage analyzed by liquid chromatography-mass spectrometry (LC-MS/MS). Here, we show that spiking small molecules, including metabolites, lipids, vitamins, and nutrients, into plasma can induce diverse protein corona patterns on otherwise identical NPs, significantly enhancing the depth of plasma proteome profiling. The protein coronas on polystyrene NPs when exposed to plasma treated with an array of small molecules (n=10) allowed for detection of 1793 proteins marking an 8.25-fold increase in the number of quantified proteins compared to plasma alone (218 proteins) and a 2.63-fold increase relative to the untreated protein corona (681 proteins). Furthermore, we discovered that adding 1000 μg/ml phosphatidylcholine could singularly increase the number of unique proteins within the protein corona (897 proteins). This specific concentration of phosphatidylcholine selectively depleted the four most abundant plasma proteins, including albumin, thus reducing concentration dynamic range of plasma proteome and boosting LC-MS/MS sensitivity for detection of proteins with lower abundance. By employing an optimized data-independent acquisition (DIA) approach, the inclusion of phosphatidylcholine led to the detection of 1436 proteins in plasma. This significant achievement is made utilizing only a single NP type and one small molecule to analyze a single plasma sample, setting a new standard in proteomic depth of the plasma sample. Given the critical role of plasma proteomics in biomarker discovery and disease monitoring, we anticipate widespread adoption of this methodology for identification and clinical translation of proteomic biomarkers into FDA approved diagnostics.

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Section: Protein Corona and Small Molecules Enable Deep Profiling Of ...mentioning

confidence: 99%

Deep Plasma Proteome Profiling by Modulating Single Nanoparticle Protein Corona with Small Molecules

Ashkarran,

Gharibi,

Modaresi

et al. 2024

Preprint

View full text Add to dashboard Cite

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Synthesis of self-targeted carbon nanodot for efficient cancer cell imaging and therapy

Noorkhajavi,

Abdian,

Najaflou

et al. 2024

Inorganic Chemistry Communications

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Interaction between carbon dots from folic acid and their cellular receptor: a qualitative physicochemical approach

Abstract: According to the World Health Organization, the number of cancers (all cancers, both sexes, all ages and worldwide) in 2020 reached a total of 19 292 789 new cases leading...

Cited by 2 publications

References 56 publications

Deep Plasma Proteome Profiling by Modulating Single Nanoparticle Protein Corona with Small Molecules

Deep Plasma Proteome Profiling by Modulating Single Nanoparticle Protein Corona with Small Molecules

Synthesis of self-targeted carbon nanodot for efficient cancer cell imaging and therapy

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