Interaction between calcofluor white and carbohydrates of α1-acid glycoprotein

Albani, Jihad René; Plancke, Yves

doi:10.1016/s0008-6215(98)00306-1

Cited by 17 publications

(3 citation statements)

References 31 publications

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“…Calcofluor White, a water‐soluble dye, was shown to exhibit selective binding to cell walls and has since been widely used as a fluorescent dye to visualise cell walls in fungi, bacteria, algae and higher plants (Darken, , ; Waaland and Waaland, ; Harrington and Hageage, ; Hoch et al ., ; Rasconi et al ., ; Zhou et al ., ; Mitra and Loque, ; Herburger and Holzinger, ; Ribas and Cortes, ; Snow, ). The staining specificity of Calcofluor White has not been resolved, although it clearly binds to cell walls composed of chitin, cellulose or other beta‐1,4‐linked carbohydrates (Maeda and Ishida, ; Albani and Plancke, ; Albani, ; Wysokowski et al ., ; Mitra and Loque, ). Calcofluor White has turned out to be a robust, cell‐wall‐specific dye and is compatible with the ClearSee protocol (Figure a), yet its excitation and emission maxima require two‐photon excitation or a 405‐nm laser that not all confocal systems are equipped with.…”

Section: Resultsmentioning

confidence: 99%

A protocol for combining fluorescent proteins with histological stains for diverse cell wall components

et al. 2018

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Higher plant function is contingent upon the complex three-dimensional (3D) architecture of plant tissues, yet severe light scattering renders deep, 3D tissue imaging very problematic. Although efforts to 'clear' tissues have been ongoing for over a century, many innovations have been made in recent years. Among them, a protocol called ClearSee efficiently clears tissues and diminishes chlorophyll autofluorescence while maintaining fluorescent proteins - thereby allowing analysis of gene expression and protein localisation in cleared samples. To further increase the usefulness of this protocol, we have developed a ClearSee-based toolbox in which a number of classical histological stains for lignin, suberin and other cell wall components can be used in conjunction with fluorescent reporter lines. We found that a number of classical dyes are highly soluble in ClearSee solution, allowing the old staining protocols to be enormously simplified; these additionally have been unsuitable for co-visualisation with fluorescent markers due to harsh fixation and clearing. Consecutive staining with several dyes allows 3D co-visualisation of distinct cell wall modifications with fluorescent proteins - used as transcriptional reporters or protein localisation tools - deep within tissues. Moreover, the protocol is easily applied on hand sections of different organs. In combination with confocal microscopy, this improves image quality while decreasing the time and cost of embedding/sectioning. It thus provides a low-cost, efficient method for studying thick plant tissues which are usually cumbersome to visualise. Our ClearSee-adapted protocols significantly improve and speed up anatomical and developmental investigations in numerous plant species, and we hope they will contribute to new discoveries in many areas of plant research.

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Section: Resultsmentioning

confidence: 99%

A protocol for combining fluorescent proteins with histological stains for diverse cell wall components

et al. 2018

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“…The effect of chitosan on the temporal development of C. albicans biofilms on cover slips was investigated with fluorescence microscopy using Calcofluor White M2R, a UV-excitable dye that binds chitin and β-glucan and has long been used to highlight the fungal cell wall (17). Fluorescence microscopy was performed to visualize and compare the three distinct developmental phases between the untreated and chitosan-treated C. albicans biofilms (Fig.…”

Section: Resultsmentioning

confidence: 99%

In vitro damage of Candida albicans biofilms by chitosan

Liu

Zheng

et al. 2014

Experimental and Therapeutic Medicine

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With the increasing usage of indwelling medical devices in clinical practice, the frequency of fungal infections has increased, such as that of Candida albicans (C. albicans). Biofilms, a protected niche for microorganisms, are resistant to a range of current antifungal agents. Chitosan is a polyatomic biopolymer with advantageous biocompatibility, biodegradation, nontoxicity and antibacterial properties. To investigate the inhibitory effect of chitosan on biofilms formed by C. albicans, cell viability, 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-caboxanilide reduction, and morphological assays, including fluorescence microscopy and scanning electron microscopy (SEM), were employed. As assessed by cell viability assay, chitosan showed significant inhibitory effects on the planktonic cells and the biofilm of C. albicans in a dose-dependent manner. Fluorescence microscopy and SEM assays confirmed that the chitosan-treated group showed delayed C. albicans biofilm formation with defect morphological features, due to the inhibitory effects of the vast majority of fungal cell growth. In conclusion, C. albicans biofilms were compromised by the treatment with chitosan, providing an alternative therapeutic strategy against the fungal biofilms in the medical devices.

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“…3F). The morphostructural organization of P. ostreatus biofilms was also investigated by means of CLSM after combined staining with Calcofluor White (CFW) and Texas Red-concanavalin A conjugate (CATR) (33). In 4-day-old biofilms, the architecture of the mycelial network was characterized by the presence of interstitial voids and water channels (Fig.…”

Section: Resultsmentioning

confidence: 99%

Characterization of Pleurotus ostreatus Biofilms by Using the Calgary Biofilm Device

Pesciaroli

Petruccioli

Fedi

et al. 2013

Appl Environ Microbiol

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The adequacy of the Calgary biofilm device, often referred to as the MBEC system, as a high-throughput approach to the production and subsequent characterization of Pleurotus ostreatus biofilms was assessed. The hydroxyapatite-coating of pegs was necessary to enable biofilm attachment, and the standardization of vegetative inocula ensured a uniform distribution of P. ostreatus biofilms, which is necessary for high-throughput evaluations of several antimicrobials and exposure conditions. Scanning electron microscopy showed surface-associated growth, the occurrence of a complex aggregated growth organized in multilayers or hyphal bundles, and the encasement of hyphae within an extracellular matrix (ECM), the extent of which increased with time. Chemical analyses showed that biofilms differed from free-floating cultures for their higher contents of total sugars (TS) and ECM, with the latter being mainly composed of TS and, to a lesser extent, protein. Confocal laser scanning microscopy analysis of 4-day-old biofilms showed the presence of interspersed interstitial voids and water channels in the mycelial network, the density and compactness of which increased after a 7-day incubation, with the novel occurrence of ECM aggregates with an ␣-glucan moiety. In 4-and 7-day-old biofilms, tolerance to cadmium was increased by factors of 3.2 and 11.1, respectively, compared to coeval free-floating counterparts.

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Interaction between calcofluor white and carbohydrates of α1-acid glycoprotein

Cited by 17 publications

References 31 publications

A protocol for combining fluorescent proteins with histological stains for diverse cell wall components

A protocol for combining fluorescent proteins with histological stains for diverse cell wall components

In vitro damage of Candida albicans biofilms by chitosan

Characterization of Pleurotus ostreatus Biofilms by Using the Calgary Biofilm Device

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