Interaction between Bombyx mori Cytoplasmic Polyhedrosis Virus NSP8 and BmAgo2 Inhibits RNA Interference and Enhances Virus Proliferation

Abstract

It has been reported that the RNAi pathway is inhibited by binding RNAi suppressors encoded by some insect-specific viruses belonging to Dicistroviridae , Nodaviridae , or Birnaviridae to dsRNAs to protect dsRNAs from being cut by Dicer-2. However, it is unknown whether BmCPV, belonging to Spinareoviridae , encodes an RNAi suppressor.

WIV, a protein domain found in a wide number of arthropod viruses, which probably facilitates infection

“…It is not known whether these functions are carried out by the N-terminal SPD-like domain of p44, by its central linker (predicted disordered), or by its C-terminal WIV domain (). The same study found that p44 coprecipitated the genomic dsRNA of cypovirus 1, suggesting that p44 binds genomic dsRNA [67]. However, earlier studies reported that p44 bound ssRNA but not dsRNA, through its central linker [68].…”

“…2d). The same study found that p44 coprecipitated the genomic dsRNA of cypovirus 1, suggesting that p44 binds genomic dsRNA [67]. However, earlier studies reported that p44 bound ssRNA but not dsRNA, through its central linker [68].…”

“…Recently, p44/NSP8 was found to suppress RNA interference (RNAi) and to interact with the protein Argonaute 2 of Bombyx mori [67], a component of the RNA-induced silencing complex RISC. It is not known whether these functions are carried out by the N-terminal SPD-like domain of p44, by its central linker (predicted disordered), or by its C-terminal WIV domain (Fig.…”