Interaction between amylose, fatty acid, and β‐lactoglobulin to study multiple biomacromolecules self‐assembly and application

Abstract

New concept for the development of supramolecular assemblies from intricate interactions between different classes of biomacromolecules (polysaccharides, proteins and lipids) is yet to come, due to their intrinsic chemical and structural complexity and incompatibility. Herein, we report an interaction mechanism among multiple biomacromolecules, and the structural and digestive properties of their assemblies using amylose (AM), lauric acid (LA), and β‐lactoglobulin (βLG) as exemplars. AM, LA, and βLG interact to form a water‐soluble ternary complex through van der Waals forces between AM and LA and high affinity binding between AM and βLG, which can further assemble into uniform‐sized, semi‐crystalline nanospheres under certain thermodynamic conditions. These nanospheres are substantially resistant to amylolysis, thus can be well utilized by gut microbiota, including increasing short‐chain fatty acid levels and shaping bacterial communities. Illustrating the complexation of AM, LA, and βLG and their assemblies from disorder to order, this work offers potential rationale of assemblies for multiple biomacromolecules driven by non‐covalent interactions and substantial potentials for supramolecular biomaterials development.

Simple Method for Preparing Starch Inclusion Complexes with Enhanced Amylolysis Resistance and Antioxidant Properties

“…Of starch-inclusion complexes, starch-lipid complexes with fatty acids, monoglycerides, and diglycerides are studied most extensively, as can be seen from published review articles. − Starch-lipid complexes have reduced susceptibility to amylolysis, which is largely determined by the aggregated structure of complexes, including the degree of V-type crystallite perfection and the aggregate morphology. − Other potential ligand molecules, especially bioactive phenolics, have also been studied for the purpose of making starch-phenolic complexes with unique functional properties. − As phenolic compounds have various bioactivities, starch-phenolic complexes, compared to starch-lipid complexes, may offer other potential health benefits than simply resistance to amylolysis . For example, complexes formed between rice starch and dodecyl gallate have good antioxidant activity as well as low enzymic digestibility .…”