Interaction between 30 S Ribosomal Components in a Viomycin Resistant Mutant of <i>Mycobacterium smegmatis</i>

Mizuguchi, Yasuo; Suga, Kiyoko; Yamada, Takeshi

doi:10.1111/j.1348-0421.1979.tb00500.x

Cited by 4 publications

(4 citation statements)

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“…Strains E, M, A, and O-1 were isolated from ATCC 14468 by Yamada et al by resistance to viomycin, but they showed pleiotropic drugresistant phenotypes (27). The mutational sites for the resistance to kanamycin, viomycin, and streptomycin of these mutants have been characterized as being on the ribosomal subunits (14,15,27). Strains AC21, AC22, and AC23 were isolated by resistance to kanamycin but showed divergent levels of resistance.…”

Section: Methodsmentioning

confidence: 99%

Molecular analysis of kanamycin and viomycin resistance in Mycobacterium smegmatis by use of the conjugation system

et al. 1997

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We examined the molecular mechanisms of resistance to kanamycin and viomycin in Mycobacterium smegmatis. All of the M. smegmatis strains with high-level kanamycin resistance had a nucleotide substitution from A to G at position 1389 of the 16S rRNA gene (rrs). This position is equivalent to position 1408 of Escherichia coli, and mutation at this position is known to cause aminoglycoside resistance. Mutations from G to A or G to T at position 1473 of the M. smegmatis rrs gene were found in viomycin-resistant mutants which had been designated vicB mutants in our earlier studies. Using the M. smegmatis conjugation system, we confirmed that these mutations indeed contributed to kanamycin and viomycin resistance, and kanamycin susceptibility was dominant over resistance in a heterogenomic strain. Additional experiments showed that three of four Mycobacterium tuberculosis strains with high-level kanamycin resistance had a mutation from A to G at position 1400, which was equivalent to position 1389 of M. smegmatis.

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Section: Methodsmentioning

confidence: 99%

Molecular analysis of kanamycin and viomycin resistance in Mycobacterium smegmatis by use of the conjugation system

et al. 1997

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“…The altered ribosomes were cross-resistant to streptomycin and kanamycin. These stu-dies suggested that the expression of high level resistance to viomycin can be generated only by a combination of several alterations of the ribosomal constituents (8)(9)(10).…”

Section: Introductionmentioning

confidence: 99%

The translocation inhibitor tuberactinomycin binds to nucleic acids and blocks the in vitro assembly of 50S subunits

et al. 1980

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Binding studies were performed with a [14C]-labelled derivative of viomycin, tuberactinomycin 0 (TUM O). TUM O bound to 30S and 50S subunits. The binding component was the RNA, since ribosomal proteins did not bind the drug. Other RNAs such as tRNA, phage RNA (MS2), and homopolynucleotides also bound the drug. Striking differences in the binding capacity of the various homopolynucleotides were found. Poly(U) bound strongly, poly(G) and poly(C) bound intermediately, whereas poly(A) showed a very low binding. DNA also bound TUM O, although with native DNA the binding was only weak. Finally the effects of viomycin on the assembly in vitro of the 50S subunit from E. coli were tested. A very strong inhibition was found: when the reconstitution was performed at 0.5 x 10(-6) M viomycin the particles formed sedimented at about 50S, but showed a residual activity of less than 10%. The inhibitory power of viomycin with respect to the in vitro assembly is more pronounced than that found in in vitro systems for protein synthesis.

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“…Furthermore, it inhibits ribosomal translocation by arresting peptidyl-tRNA in the ribosomal acceptor site (23). Additionally, changes in the 30S and 50S ribosomal subunits alter binding and confer viomycin resistance in M. smegmatis (8,22,33,40,41,43).…”

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Mutation of tlyA Confers Capreomycin Resistance in Mycobacterium tuberculosis

Maus

Plikaytis

Shinnick

2005

Antimicrob Agents Chemother

202

159

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Interaction between 30 S Ribosomal Components in a Viomycin Resistant Mutant of Mycobacterium smegmatis

Cited by 4 publications

References 8 publications

Molecular analysis of kanamycin and viomycin resistance in Mycobacterium smegmatis by use of the conjugation system

Molecular analysis of kanamycin and viomycin resistance in Mycobacterium smegmatis by use of the conjugation system

The translocation inhibitor tuberactinomycin binds to nucleic acids and blocks the in vitro assembly of 50S subunits

Mutation of tlyA Confers Capreomycin Resistance in Mycobacterium tuberculosis

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