Interaction and incorporation of ovalbumin with stearic acid monolayer: Langmuir–Blodgett film formation and deposition

Adsorption of pepsin in octadecylamine matrix at air–water interface

“…A slow protein attachment process is also competing here. Similar observations were found for ovalbumin in DPPC, ODA and SA [10,17,20] as well as puroindoline in DPPC and in DPPG monolayer [25].…”

“…For analyzing the kinetics of PEP association, the adsorption curves are fitted to double exponential association mechanism using following equation [17,20,26].…”

“…octadecylamine) has been used to immobilize proteins in many investigations [1,[17][18][19][20]. However, the conformational regulation and orientation of protein/enzyme in octadecylamine (ODA) matrix is not well established.…”

Incorporation of ovalbumin within cationic octadecylamine monolayer and a comparative study with zwitterionic DPPC and anionic stearic acid monolayer

“…A surface compressibility study was also done to characterize the states of ODA-OVA. The present results of π -A, π -t and the compressibility data are compared with the DPPC-OVA [16] and SA-OVA [17] systems. Steady state fluorescence spectroscopy was used to study the tryptophan fluorescence of the protein at different environmental condition.…”

“…In our recent communications [16,17], the interaction of soluble surface-active protein, the ovalbumin (OVA) with zwitterionic 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and anionic stearic acid (SA) monolayer has been studied at air/water interface. It is to be emphasized that in those cases the incorporation or adsorption of protein were found to be different.…”