Interaction among cultural factors in microtuberization of white yam (Dioscorea rotundata)

Balogun, M. O.; Fawole, I.; Ng, S.Y.C.; Ng, N.Q.; Shiwachi, Hironobu; Kikuno, Hidehiko

doi:10.1002/ts.61

Cited by 14 publications

(9 citation statements)

References 6 publications

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“…In the various Dioscorea species, different in vitro growth conditions have been reported as being optimum, mainly differing at the level of the carbon sources and growth regulator concentrations (Malaurie et al 1995a), and incubation conditions (Balogun et al 2006). We have shown that an increase of the sucrose concentration from 3% to 8% induced a significant increase of root (Fig.…”

Section: Discussionmentioning

confidence: 89%

Axillary proliferation and tuberisation of Dioscorea cayenensis–D. rotundata complex

Ovono

Kevers

Dommès

2007

Plant Cell Tiss Organ Cult

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Yams (Dioscorea spp) are tuber crops used as staple food in Africa because of their nutritional value. However agronomic constraints, phytosanitary problems and the lack of good healthy planting material restrict their production. In contrast to the inefficiency of traditional method of planting, tissue culture techniques allow to increase the multiplication and the rapid production of pathogenfree plant material. This work was undertaken to provide farmers in African countries with healthy microplants and microtubers as seeds. In vitro nodal segments of two varieties of local yams D. cayenensis-D. rotundata complex (cv. 'Singo', cv. 'Singou' and cv. 'Gnidou') were micropropagated on the modified medium of Murashige and Skoog. The morphogenesis, the growth of microplants and microtuber formation have been found to be controlled by external factors that act individually and synergistically. Addition of kinetin (2 mg l À1 ) to the culture media could reduce multiplication rate (node number) of some clones. An increase of the sucrose concentration from 3% to 5% induced no change in the multiplication and tuberisation parameters. An important reduction of the multiplication (shoot number, height and node number) and the tuberisation (tuber number and length) was observed with 8% sucrose. Multiplication (shoot and node number) was increased in the presence of jasmonic acid (10 mM). JA also induced an increase of tuber number in the absence of Kin. Multiplication of yam by in vitro growth of nodal segments is a way for rapid clonal multiplication and could allow solving the problem of lack of seed material faced by farmers. This method could also be used for multiplication of elite cultivars, independently of the growing season.

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Section: Discussionmentioning

confidence: 89%

Axillary proliferation and tuberisation of Dioscorea cayenensis–D. rotundata complex

Ovono

Kevers

Dommès

2007

Plant Cell Tiss Organ Cult

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“…Growth of plantlets and tuberization in the third treatment were also significantly (P B 0.05) higher than in the first treatment (Table 1). Many plant species are easy to propagate in liquid medium compared to semi-solid or solid medium, which had been attributed to relatively ease of absorption of nutrients in the former (Balogun et al 2006). Yam clones ''Pacala Duclos'' and ''Belep'' in temporary immersion system also showed favorable results on shoot growth stage and in the development of microtubers in comparison with solid culture medium (Jova et al 2005).…”

Section: Resultsmentioning

confidence: 91%

“…and D. cayenensis Lam. (Viana and Mantell 1989), D. rotundata (Balogun et al 2006), D. nipponica Makino. (Chen et al 2007) and the D. cayenensis-D. rotundata complex (Ovono et al 2007(Ovono et al , 2009(Ovono et al , 2010a.…”

Section: Introductionmentioning

confidence: 99%

Axillary shoot proliferation and tuberization of Dioscorea fordii Prain et Burk

Yan

Yang

2010

Plant Cell Tiss Organ Cult

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Dioscorea fordii Prain et Burk., a member of the family Dioscoreaceae, is an important tuberous food in China owing to its edible and medicinal functions. However, the lack of healthy planting material has restricted its production. The present study was an attempt to develop a protocol for in vitro propagation of D. fordii. In an initial two by two (2 2 ) factorial experiment, the effects of culture system and activated charcoal have been observed for axillary shoot proliferation and tuberization of in vitro plantlets. Shoot length, frequency of proliferation, fresh weight and dry weight of shoots, frequency of tuberization and mean number of tubers per plantlet (NTPs) were significantly (P B 0.05) greater in liquid medium compared to semi-solid media. Secondly, an orthogonal experimental design [L 9 (3 4 )] was used to investigate the effects of the ratio of naphthalene acetic acid (NAA) to 6-benzyladenine (BA) (NAA/BA), paclobutrazol, methyl jasmonate and sucrose concentration on tuberization. Sucrose concentration had the record effect on frequency of tuberization, NTPs and frequency of proliferation. The preferred medium for axillary shoot proliferation and tuberization of D. fordii found to be MS basal medium (Murashige and Skoog in Physiol Plant 15:473-479, 1962) supplemented with 1.0 mg l -1 BA, 0.1 mg l -1 NAA, 30 g l -1 sucrose and 1.5 g l -1 AC in liquid culture.

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“…Microtubers have a potential to be integrated into seed yam programs (Balogun et al, 2006;Chen et al, 2007). Microtubers are particularly convenient for handling, storage and distribution.…”

Section: Introductionmentioning

confidence: 99%

“…Unlike micro propagated plantlets, they do not need a time consuming hardening period in a greenhouse and may be adapted easily to largescale mechanised planting in the field (Coleman et al, 2001;Pruski et al, 2003). Micro propagation of Dioscorea alata L. in semi-solid medium and in conventional size culture flasks is characterized by low microtuber formation frequencies by in vitro plants and microtubers with average fresh weight lower than 0.5 gFW (Balogun et al, 2006;Ondo et al, 2007;Balogun, 2009). These factors limit its use as propagule for propagation, microtuber formation and direct planting under field conditions.…”

Section: Introductionmentioning

confidence: 99%

Performance of yam microtubers from temporary immersion system in field conditions

Manuel¹,

Rafael²,

Ayme³

et al. 2011

Afr. J. Biotechnol.

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The yam clones ´Pacala Duclos´ and ´Belep´ of Dioscorea alata were used to evaluate the performance of microtubers formed in temporary immersion systems (TIS) in field conditions. Previously sprouted microtubers with a fresh weight higher than 3.0 gFW were used while in vitro plants and tuber crowns from conventional propagation methods served as control. In both clones there were no significant differences in qualitative morphological characters between plants from microtubers and in vitro plants for all traits but both differed significantly from plants obtained from tuber crowns. The same trend was observed for number, length, diameter and fresh weight of tubers produced 36 weeks after field planting. The number of tubers formed per plant raised from microtubers doubled that raised from tuber crowns in both clones. Microtubers from temporary immersion systems can be grown on the field and used in original seed production programs.

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Interaction among cultural factors in microtuberization of white yam (Dioscorea rotundata)

Cited by 14 publications

References 6 publications

Axillary proliferation and tuberisation of Dioscorea cayenensis–D. rotundata complex

Axillary proliferation and tuberisation of Dioscorea cayenensis–D. rotundata complex

Axillary shoot proliferation and tuberization of Dioscorea fordii Prain et Burk

Performance of yam microtubers from temporary immersion system in field conditions

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