Interacting Partners of M-PMV Nucleocapsid-DUTPase

Németh-Pongrácz, V.; Snášel, Jan; Rumlová, Michaela; Pichová, Iva; Vértessy, Beáta G.

doi:10.1080/15257770600894535

Cited by 1 publication

(2 citation statements)

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“…This collection of activities is likely to substantially affect the mature beta-retroviral dUTPases (that were shown always to retain the NC). Indeed, three retroviral proteins, IN, capsid and NC, were found to be capable of physical interaction with MPMV dUTPase [ 64 ]. This protein is present in stable form that resists proteolysis by retroviral and cellular proteases in virions as well as in virus-infected cells.…”

Section: Reviewmentioning

confidence: 99%

“…Such a co-folding of the dUTPase terminal segments, which was not observed in other dUTPases, results from the presence of the fused NC domain [ 63 ]. Elaborate studies on MPMV dUTPases were conducted to show the mechanism of the alpha attack-mediated dUTP hydrolysis that is carried out by the enzyme [ 64 ]. Here, a combination of diverse structural methods, as well as the knowledge of other dUTPases, unveiled molecular details of the catalytic nucleophilic attack and identified novel enzyme-product intermediates.…”

Section: Reviewmentioning

confidence: 99%

See 1 more Smart Citation

dUTPase: the frequently overlooked enzyme encoded by many retroviruses

Hizi

Herzig

2015

Retrovirology

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Retroviruses are among the best studied viruses in last decades due to their pivotal involvement in cellular processes and, most importantly, in causing human diseases, most notably—acquired immunodeficiency syndrome (AIDS) that is triggered by human immunodeficiency viruses types 1 and 2 (HIV-1 and HIV-2, respectively). Numerous studied were conducted to understand the involvement of the three cardinal retroviral enzymes, reverse transcriptase, integrase and protease, in the life cycle of the viruses. These studies have led to the development of many inhibitors of these enzymes as anti-retroviral specific drugs that are used for routine treatments of HIV/AIDS patients. Interestingly, a fourth virus-encoded enzyme, the deoxyuridine 5′-triphosphate nucleotidohydrolase (dUTPase) is also found in several major retroviral groups. The presence and the importance of this enzyme to the life cycle of retroviruses were usually overlooked by most retrovirologists, although the occurrence of dUTPases, particularly in beta-retroviruses and in non-primate retroviruses, is known for more than 20 years. Only more recently, retroviral dUTPases were brought into the limelight and were shown in several cases to be essential for viral replication. Therefore, it is likely that future studies on this enzyme will advance our knowledge to a level that will allow designing novel, specific and potent anti-dUTPase drugs that are effective in combating retroviral diseases. The aim of this review is to give concise background information on dUTPases in general and to summarize the most relevant data on retroviral dUTPases and their involvement in the replication processes and pathogenicity of the viruses, as well as in possibly-associated human diseases.

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Section: Reviewmentioning

confidence: 99%

Section: Reviewmentioning

confidence: 99%